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    Engineered CO2-fixing chemotrophic microorganisms producing carbon-based products and methods of using the same
    1.
    发明授权
    Engineered CO2-fixing chemotrophic microorganisms producing carbon-based products and methods of using the same 有权

    公开(公告)号：US09957534B2

    公开(公告)日：2018-05-01

    申请号：US15233512

    申请日：2016-08-10

    Applicant: Kiverdi, Inc.

    Inventor： Itzhak Kurek ,  John S. Reed ,  Lisa Dyson ,  Henrik Fyrst ,  Christer Jansson ,  David Galgoczy

    IPC: C12N1/20 ,  C12P7/64 ,  C07H21/04 ,  C12N9/16 ,  C12N9/02

    CPC classification number: C12P7/649 ,  C12N1/20 ,  C12N9/0008 ,  C12N9/0083 ,  C12N9/16 ,  C12N9/88 ,  C12P5/002 ,  C12P5/026 ,  C12P7/04 ,  C12P7/6409 ,  C12Y114/99033 ,  C12Y301/02014 ,  Y02E50/13 ,  Y02P20/52

    Abstract: Disclosed herein are microorganisms containing exogenous or heterologous nucleic acid sequences, wherein the microorganisms are capable of growing on gaseous carbon dioxide, gaseous hydrogen, syngas, or combinations thereof. In some embodiments the microorganisms are chemotrophic bacteria that produce or secrete at least 10% of lipid by weight. Also disclosed are methods of fixing gaseous carbon into organic carbon molecules useful for industrial processes. Also disclosed are methods of manufacturing chemicals or producing precursors to chemicals useful in jet fuel, diesel fuel, and biodiesel fuel. Exemplary chemicals or precursors to chemicals useful in fuel production are alkanes, alkenes, alkynes, fatty acid alcohols, fatty acid aldehydes, desaturated hydrocarbons, unsaturated fatty acids, hydroxyl acids, or diacids with carbon chains between six and thirty carbon atoms long. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of butanediol and its chemical precursors in low-oxygen or anaerobic fermentation. Also disclosed are microorganisms and methods using disclosed microorganisms for generating hydroxylated fatty acids in microbes through the transfer of enzymes that are known to hydroxylate fatty acids in plants or microbes. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of shorter-chain fatty acids in microbes through the introduction of exogenous fatty acyl-CoA binding proteins.

    Engineered CO2-Fixing Chemotrophic Microorganisms Producing Carbon-Based Products and Methods of Using the Same
    2.
    发明申请
    Engineered CO2-Fixing Chemotrophic Microorganisms Producing Carbon-Based Products and Methods of Using the Same 审中-公开

    公开(公告)号：US20190040427A1

    公开(公告)日：2019-02-07

    申请号：US15936440

    申请日：2018-03-27

    Applicant: Kiverdi, Inc.

    Inventor： Itzhak Kurek ,  John S. Reed ,  Lisa Dyson ,  Henrik Fyrst ,  Christer Jansson ,  David Galgoczy

    IPC: C12P7/64 ,  C12P5/00 ,  C12N1/20 ,  C12P7/04 ,  C12P5/02 ,  C12N9/88 ,  C12N9/16 ,  C12N9/02

    Abstract: Disclosed herein are microorganisms containing exogenous or heterologous nucleic acid sequences, wherein the microorganisms are capable of growing on gaseous carbon dioxide, gaseous hydrogen, syngas, or combinations thereof. In some embodiments the microorganisms are chemotrophic bacteria that produce or secrete at least 10% of lipid by weight. Also disclosed are methods of fixing gaseous carbon into organic carbon molecules useful for industrial processes. Also disclosed are methods of manufacturing chemicals or producing precursors to chemicals useful in jet fuel, diesel fuel, and biodiesel fuel. Exemplary chemicals or precursors to chemicals useful in fuel production are alkanes, alkenes, alkynes, fatty acid alcohols, fatty acid aldehydes, desaturated hydrocarbons, unsaturated fatty acids, hydroxyl acids, or diacids with carbon chains between six and thirty carbon atoms long. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of butanediol and its chemical precursors in low-oxygen or anaerobic fermentation. Also disclosed are microorganisms and methods using disclosed microorganisms for generating hydroxylated fatty acids in microbes through the transfer of enzymes that are known to hydroxylate fatty acids in plants or microbes. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of shorter-chain fatty acids in microbes through the introduction of exogenous fatty acyl-CoA binding proteins.

    Engineered CO2-Fixing Chemotrophic Microorganisms Producing Carbon-Based Products and Methods of Using the Same
    3.
    发明申请
    Engineered CO2-Fixing Chemotrophic Microorganisms Producing Carbon-Based Products and Methods of Using the Same 审中-公开

    公开(公告)号：US20170152533A1

    公开(公告)日：2017-06-01

    申请号：US15233512

    申请日：2016-08-10

    Applicant: Kiverdi, Inc.

    Inventor： Itzhak Kurek ,  John S. Reed ,  Lisa Dyson ,  Henrik Fyrst ,  Christer Jansson ,  David Galgoczy

    IPC: C12P7/64 ,  C12N9/02 ,  C12N9/16

    CPC classification number: C12P7/649 ,  C12N1/20 ,  C12N9/0008 ,  C12N9/0083 ,  C12N9/16 ,  C12N9/88 ,  C12P5/002 ,  C12P5/026 ,  C12P7/04 ,  C12P7/6409 ,  C12Y114/99033 ,  C12Y301/02014 ,  Y02E50/13 ,  Y02P20/52

    Abstract: Disclosed herein are microorganisms containing exogenous or heterologous nucleic acid sequences, wherein the microorganisms are capable of growing on gaseous carbon dioxide, gaseous hydrogen, syngas, or combinations thereof. In some embodiments the microorganisms are chemotrophic bacteria that produce or secrete at least 10% of lipid by weight. Also disclosed are methods of fixing gaseous carbon into organic carbon molecules useful for industrial processes. Also disclosed are methods of manufacturing chemicals or producing precursors to chemicals useful in jet fuel, diesel fuel, and biodiesel fuel. Exemplary chemicals or precursors to chemicals useful in fuel production are alkanes, alkenes, alkynes, fatty acid alcohols, fatty acid aldehydes, desaturated hydrocarbons, unsaturated fatty acids, hydroxyl acids, or diacids with carbon chains between six and thirty carbon atoms long. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of butanediol and its chemical precursors in low-oxygen or anaerobic fermentation. Also disclosed are microorganisms and methods using disclosed microorganisms for generating hydroxylated fatty acids in microbes through the transfer of enzymes that are known to hydroxylate fatty acids in plants or microbes. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of shorter-chain fatty acids in microbes through the introduction of exogenous fatty acyl-CoA binding proteins.

    Engineered CO2-Fixing Chemotrophic Microorganisms Producing Carbon-Based Products and Methods of Using the Same
    6.
    发明申请
    Engineered CO2-Fixing Chemotrophic Microorganisms Producing Carbon-Based Products and Methods of Using the Same 审中-公开
    工程化的CO2固定化学生物微生物生产碳基产品及其使用方法

    公开(公告)号：US20150017694A1

    公开(公告)日：2015-01-15

    申请号：US14388756

    申请日：2013-03-15

    Applicant: Kiverdi, Inc.

    Inventor： Itzhak Kurek ,  John S. Reed ,  Lisa Dyson ,  Michael Siani-Rose ,  Henrik Fyrst ,  Christer Jansson ,  David Galgoczy

    IPC: C12P7/64 ,  C12N15/74

    CPC classification number: C12P7/649 ,  C12N1/20 ,  C12N9/0008 ,  C12N9/0083 ,  C12N9/16 ,  C12N9/88 ,  C12P5/002 ,  C12P5/026 ,  C12P7/04 ,  C12P7/6409 ,  C12Y114/99033 ,  C12Y301/02014 ,  Y02E50/13 ,  Y02P20/52

    Abstract: Disclosed herein are microorganisms containing exogenous or heterologous nucleic acid sequences, wherein the microorganisms are capable of growing on gaseous carbon dioxide, gaseous hydrogen, syngas, or combinations thereof. In some embodiments the microorganisms are chemotrophic bacteria that produce or secrete at least 10% of lipid by weight. Also disclosed are methods of fixing gaseous carbon into organic carbon molecules useful for industrial processes. Also disclosed are methods of manufacturing chemicals or producing precursors to chemicals useful in jet fuel, diesel fuel, and biodiesel fuel. Exemplary chemicals or precursors to chemicals useful in fuel production are alkanes, alkenes, alkynes, fatty acid alcohols, fatty acid aldehydes, desaturated hydrocarbons, unsaturated fatty acids, hydroxyl acids, or diacids with carbon chains between six and thirty carbon atoms long. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of butanediol and its chemical precursors in low-oxygen or anaerobic fermentation. Also disclosed are microorganisms and methods using disclosed microorganisms for generating hydroxylated fatty acids in microbes through the transfer of enzymes that are known to hydroxylate fatty acids in plants or microbes. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of shorter-chain fatty acids in microbes through the introduction of exogenous fatty acyl-CoA binding proteins.

    Abstract translation: 本文公开了含有外源或异源核酸序列的微生物，其中微生物能够在气态二氧化碳，气态氢，合成气或其组合上生长。 在一些实施方案中，微生物是产生或分泌至少10重量％脂质的化学营养细菌。 还公开了将气态碳固定在可用于工业过程的有机碳分子中的方法。 还公开了制造化学品或生产用于喷气燃料，柴油燃料和生物柴油燃料的化学品的前体的方法。 用于燃料生产的化学品或化学物质的示例是烷烃，烯烃，炔烃，脂肪酸醇，脂肪酸醛，去饱和烃，不饱和脂肪酸，羟基酸或具有6-30个碳原子长的碳链的二酸。 还公开了使用公开的微生物在低氧或厌氧发酵中生产丁二醇及其化学前体的微生物和方法。 还公开了微生物和使用公开的微生物通过转移在植物或微生物中羟基化脂肪酸已知的酶在微生物中产生羟化脂肪酸的微生物和方法。 还公开了微生物和使用公开的微生物通过引入外源性脂肪酰基-CoA结合蛋白在微生物中生产短链脂肪酸的微生物和方法。

    PROCESS FOR GROWING NATURAL OR ENGINEERED HIGH LIPID ACCUMULATING STRAIN ON CRUDE GLYCEROL AND/OR OTHER SOURCES OF WASTE CARBON FOR THE PRODUCTION OF OILS, FUELS, OLEOCHEMICALS, AND OTHER VALUABLE ORGANIC COMPOUNDS
    9.
    发明申请
    PROCESS FOR GROWING NATURAL OR ENGINEERED HIGH LIPID ACCUMULATING STRAIN ON CRUDE GLYCEROL AND/OR OTHER SOURCES OF WASTE CARBON FOR THE PRODUCTION OF OILS, FUELS, OLEOCHEMICALS, AND OTHER VALUABLE ORGANIC COMPOUNDS 审中-公开
    用于生产自然或工程高脂肪累积菌株的原料甘油和/或其他废物来源用于生产油，燃料，化学品和其他有价值的有机化合物的方法

    公开(公告)号：US20150140640A1

    公开(公告)日：2015-05-21

    申请号：US14361603

    申请日：2012-11-29

    Applicant: Kiverdi, Inc.

    Inventor： John S. Reed ,  Peter Dalla-Betta ,  Itzhak Kurek ,  Christer Jansson ,  Michael Siani-Rose ,  Lisa Dyson

    IPC: C12N1/20

    CPC classification number: C12N1/20 ,  C12N9/0008 ,  C12N9/16 ,  C12N9/88 ,  C12P5/002 ,  C12P5/026 ,  C12P7/04 ,  C12P7/6409 ,  C12P7/6463 ,  C12P7/649 ,  Y02E50/13

    Abstract: Disclosed herein are microorganisms capable of growing on crude glycerol and/or glycerol and/or methanol, or combinations thereof. In some embodiments the microorganisms are knallgas bacteria that produce or secrete at least 10% of lipid by weight. Also disclosed are methods of converting crude glycerol and/or glycerol and/or methanol produced as byproduct of processes including but not limited to biodiesel production, into organic carbon molecules such as triacylglycerol useful for industrial processes including but not limited to the production of additional biodiesel. Also disclosed are methods of manufacturing chemicals or producing precursors to chemicals useful in oleochemicals, jet fuel, diesel fuel, and biodiesel fuel. Exemplary chemicals or precursors to chemicals useful in fuel and/or oleochemical production are alkanes, alkenes, alkynes, fatty acid alcohols, fatty acid aldehydes, methyl esters, ethyl esters, alkyl esters, with carbon chains between five and twenty four carbon atoms long.

    Abstract translation: 本文公开了能够生长在粗甘油和/或甘油和/或甲醇或其组合上的微生物。 在一些实施方案中，微生物是产生或分泌至少10重量％的脂质的疱疹细菌。 还公开了将作为包括但不限于生物柴油生产的方法的副产物生产的粗甘油和/或甘油和/或甲醇转化为有机碳分子，例如可用于工业过程的有机碳分子，例如三酰基甘油，包括但不限于生产额外的生物柴油 。 还公开了制造化学品或生产用于油化学品，喷气燃料，柴油燃料和生物柴油燃料的化学品的前体的方法。 用于燃料和/或油脂化学生产的化学物质的示例性化学物质或前体是烷烃，烯烃，炔烃，脂肪酸醇，脂肪酸醛，甲酯，乙酯，烷基酯，碳链长度在五至二十四个碳原子之间。

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