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    Engineered CO2-fixing chemotrophic microorganisms producing carbon-based products and methods of using the same
    4.
    发明授权
    Engineered CO2-fixing chemotrophic microorganisms producing carbon-based products and methods of using the same 有权

    公开(公告)号：US09957534B2

    公开(公告)日：2018-05-01

    申请号：US15233512

    申请日：2016-08-10

    Applicant: Kiverdi, Inc.

    Inventor： Itzhak Kurek ,  John S. Reed ,  Lisa Dyson ,  Henrik Fyrst ,  Christer Jansson ,  David Galgoczy

    IPC: C12N1/20 ,  C12P7/64 ,  C07H21/04 ,  C12N9/16 ,  C12N9/02

    CPC classification number: C12P7/649 ,  C12N1/20 ,  C12N9/0008 ,  C12N9/0083 ,  C12N9/16 ,  C12N9/88 ,  C12P5/002 ,  C12P5/026 ,  C12P7/04 ,  C12P7/6409 ,  C12Y114/99033 ,  C12Y301/02014 ,  Y02E50/13 ,  Y02P20/52

    Abstract: Disclosed herein are microorganisms containing exogenous or heterologous nucleic acid sequences, wherein the microorganisms are capable of growing on gaseous carbon dioxide, gaseous hydrogen, syngas, or combinations thereof. In some embodiments the microorganisms are chemotrophic bacteria that produce or secrete at least 10% of lipid by weight. Also disclosed are methods of fixing gaseous carbon into organic carbon molecules useful for industrial processes. Also disclosed are methods of manufacturing chemicals or producing precursors to chemicals useful in jet fuel, diesel fuel, and biodiesel fuel. Exemplary chemicals or precursors to chemicals useful in fuel production are alkanes, alkenes, alkynes, fatty acid alcohols, fatty acid aldehydes, desaturated hydrocarbons, unsaturated fatty acids, hydroxyl acids, or diacids with carbon chains between six and thirty carbon atoms long. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of butanediol and its chemical precursors in low-oxygen or anaerobic fermentation. Also disclosed are microorganisms and methods using disclosed microorganisms for generating hydroxylated fatty acids in microbes through the transfer of enzymes that are known to hydroxylate fatty acids in plants or microbes. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of shorter-chain fatty acids in microbes through the introduction of exogenous fatty acyl-CoA binding proteins.

    Microbial Conversion of CO2 and Other C1 Substrates to Protein and Meat Substitute Products
    8.
    发明申请
    Microbial Conversion of CO2 and Other C1 Substrates to Protein and Meat Substitute Products 审中-公开

    公开(公告)号：US20200216797A1

    公开(公告)日：2020-07-09

    申请号：US16716398

    申请日：2019-12-16

    Applicant: Kiverdi, Inc.

    Inventor： Lisa Dyson ,  John Reed ,  Jil Geller ,  Sonali Hande

    IPC: C12N1/20 ,  C12P21/02 ,  C12P13/04 ,  C12P7/64 ,  C12P1/04

    Abstract: Microorganisms and bioprocesses are provided that convert gaseous substrates, such as renewable H2 and waste CO2 producer gas, or syngas into high-protein biomass that may be used directly for human nutrition, or as a nutrient for plants, fungi, or other microorganisms, or as a source of soil carbon, nitrogen, and other mineral nutrients. Renewable H2 used in the processes described herein may be generated by electrolysis using solar or wind power. Producer gas used in the processes described herein may be derived from sources that include gasification of waste feedstock and/or biomass residue, waste gas from industrial processes, or natural gas, biogas, or landfill gas.

    Engineered CO2-Fixing Chemotrophic Microorganisms Producing Carbon-Based Products and Methods of Using the Same
    9.
    发明申请
    Engineered CO2-Fixing Chemotrophic Microorganisms Producing Carbon-Based Products and Methods of Using the Same 审中-公开

    公开(公告)号：US20190040427A1

    公开(公告)日：2019-02-07

    申请号：US15936440

    申请日：2018-03-27

    Applicant: Kiverdi, Inc.

    Inventor： Itzhak Kurek ,  John S. Reed ,  Lisa Dyson ,  Henrik Fyrst ,  Christer Jansson ,  David Galgoczy

    IPC: C12P7/64 ,  C12P5/00 ,  C12N1/20 ,  C12P7/04 ,  C12P5/02 ,  C12N9/88 ,  C12N9/16 ,  C12N9/02

    Abstract: Disclosed herein are microorganisms containing exogenous or heterologous nucleic acid sequences, wherein the microorganisms are capable of growing on gaseous carbon dioxide, gaseous hydrogen, syngas, or combinations thereof. In some embodiments the microorganisms are chemotrophic bacteria that produce or secrete at least 10% of lipid by weight. Also disclosed are methods of fixing gaseous carbon into organic carbon molecules useful for industrial processes. Also disclosed are methods of manufacturing chemicals or producing precursors to chemicals useful in jet fuel, diesel fuel, and biodiesel fuel. Exemplary chemicals or precursors to chemicals useful in fuel production are alkanes, alkenes, alkynes, fatty acid alcohols, fatty acid aldehydes, desaturated hydrocarbons, unsaturated fatty acids, hydroxyl acids, or diacids with carbon chains between six and thirty carbon atoms long. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of butanediol and its chemical precursors in low-oxygen or anaerobic fermentation. Also disclosed are microorganisms and methods using disclosed microorganisms for generating hydroxylated fatty acids in microbes through the transfer of enzymes that are known to hydroxylate fatty acids in plants or microbes. Also disclosed are microorganisms and methods using disclosed microorganisms for the production of shorter-chain fatty acids in microbes through the introduction of exogenous fatty acyl-CoA binding proteins.

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