-
公开(公告)号:US4560654A
公开(公告)日:1985-12-24
申请号:US261557
申请日:1981-05-07
申请人: Kiyoshi Miwa , Mahito Terabe , Masaaki Ishida , Hiroshi Matsui , Haruo Momose
发明人: Kiyoshi Miwa , Mahito Terabe , Masaaki Ishida , Hiroshi Matsui , Haruo Momose
IPC分类号: C12N15/09 , C12N15/52 , C12N15/77 , C12P13/08 , C12P19/34 , C12R1/13 , C12R1/15 , C12N1/20 , C12N15/00
CPC分类号: C12N15/52 , C12N15/77 , C12P13/08 , Y10S435/84 , Y10S435/843
摘要: An L-lysine producing microorganism which is constructed by incorporation into a recipient strain of the genus Brevibacterium or Corynebacterium of a hybrid plasmid having inserted therein a DNA fragment which is derived from a donor strain of the genus Brevibacterium or Corynebacterium and which controls resistance to S-(2-aminoethyl)-cysteine and productivity of L-lysine, is useful for the production of high levels of L-lysine by fermentation.
摘要翻译: 通过掺入到短杆菌属或棒状杆菌属的受体菌株中的杂交质粒构建的L-赖氨酸生产微生物,其中插入有衍生自短杆菌属或棒状杆菌属的供体菌株并控制对S的抗性的DNA片段 - (2-氨基乙基) - 半胱氨酸和L-赖氨酸的生产率可用于通过发酵生产高水平的L-赖氨酸。
-
公开(公告)号:US4778762A
公开(公告)日:1988-10-18
申请号:US434853
申请日:1982-10-18
申请人: Kiyoshi Miwa , Mahito Terabe , Takayasu Tsuchida , Masaaki Ishida , Shigeru Nakamori , Konosuke Sano , Haruo Momose
发明人: Kiyoshi Miwa , Mahito Terabe , Takayasu Tsuchida , Masaaki Ishida , Shigeru Nakamori , Konosuke Sano , Haruo Momose
IPC分类号: C12P13/08 , C12P13/14 , C12N15/00 , C12N1/20 , C12P19/34 , C12P21/00 , C12P21/02 , C12R1/13 , C12R1/15
CPC分类号: C12R1/13 , C12P13/08 , C12P13/14 , C12R1/15 , Y10S435/84 , Y10S435/843
摘要: A substantially pure plasmid which is characterized by a molecular weight of 3.0.+-.0.1 megadalton and the restriction endonuclease-cleavage map shown in the Figure. This plasmid is capable of propagating in Corynebacteria and due to its size is useful as a vector for the cloning of exogenous genes.
摘要翻译: 一种基本上纯的质粒,其特征在于分子量为3.0 +/- 0.1兆道尔顿和限制性内切核酸酶切割图,如图所示。 该质粒能够在棒状杆菌中繁殖,并且由于其大小可用作克隆外源基因的载体。
-
公开(公告)号:US4430430A
公开(公告)日:1984-02-07
申请号:US272286
申请日:1981-06-10
申请人: Haruo Momose , Masaaki Ishida , Mahito Terabe
发明人: Haruo Momose , Masaaki Ishida , Mahito Terabe
CPC分类号: C12N15/52 , C12P13/10 , Y10S435/849
摘要: An L-arginine producing microorganism which is constructed by transforming into a recipient strain of the genus Escherichia of a vector having inserted therein an arg A gene derived from a chromosomal DNA of the genus Escherichia, is useful for the production of high levels of L-arginine by fermentation.
摘要翻译: 通过将具有插入其中衍生自埃希氏菌属的染色体DNA的arg A基因的载体转化入埃氏杆菌属的受体菌株构建的L-精氨酸生产微生物可用于生产高水平的L- 精氨酸通过发酵。
-
公开(公告)号:US4514502A
公开(公告)日:1985-04-30
申请号:US386980
申请日:1982-06-10
申请人: Kiyoshi Miwa , Mahito Terabe , Koichi Ito , Masaaki Ishida , Kazuhiko Matsui , Shigeru Makamori , Konosuke Sano
发明人: Kiyoshi Miwa , Mahito Terabe , Koichi Ito , Masaaki Ishida , Kazuhiko Matsui , Shigeru Makamori , Konosuke Sano
IPC分类号: C12N15/09 , C07H21/04 , C12N15/52 , C12N15/75 , C12N15/77 , C12P13/22 , C12N1/20 , C12N1/00 , C12N15/00 , C12R1/15
CPC分类号: C12N15/52 , C12N15/75 , C12N15/77 , C12P13/222 , Y10S435/843
摘要: A composite plasmid which comprises (A) a drive-unit region derived from a plasmid a capable of propagating in a Coryneform glutamic acid-producing bacterium, and (B) a gene fragment derived from a plasmid b capable of propagating in Escherichia coli or Bacillus subtilis and having at least a region expressing drug resistance.
摘要翻译: 一种复合质粒,其包含(A)衍生自能够在棒杆菌型谷氨酸生产细菌中繁殖的质粒的驱动单位区域,和(B)源自能够在大肠杆菌或芽孢杆菌中扩散的质粒b的基因片段 并且至少具有表达耐药性的区域。
-
-
-