公开(公告)号：US20120142103A1

公开(公告)日：2012-06-07

申请号：US13321130

申请日：2010-05-18

申请人： Kohji Nishida ,  Ryuhei Hayashi ,  Miharu Sakurai ,  Tomofumi Kageyama ,  Shinya Yamanaka ,  Keisuke Okita

发明人： Kohji Nishida ,  Ryuhei Hayashi ,  Miharu Sakurai ,  Tomofumi Kageyama ,  Shinya Yamanaka ,  Keisuke Okita

IPC分类号： C12N5/071

CPC分类号： A61L27/3834 ,  A61L27/3869 ,  A61L27/3895 ,  C12N5/0621 ,  C12N5/0629 ,  C12N2501/01 ,  C12N2501/11 ,  C12N2501/155 ,  C12N2501/385 ,  C12N2501/39 ,  C12N2501/395 ,  C12N2502/1323 ,  C12N2502/1394 ,  C12N2506/45

摘要： The present invention relates to: a method for inducing differentiation into an epithelial progenitor cell/stem cell population or a corneal epithelial cell population by culturing, under particular conditions, induced pluripotent stem cells induced from mammalian somatic cells or undifferentiated stem cells; an epithelial progenitor cell/stem cell population or a corneal epithelial cell population obtained by the method; and a cell preparation for the treatment of epithelial disease and a cell sheet, which are prepared using these cell populations.

摘要翻译： 本发明涉及：通过在特定条件下培养从哺乳动物体细胞或未分化干细胞诱导的多能干细胞诱导分化成上皮祖细胞/干细胞群或角膜上皮细胞群的方法; 通过该方法获得的上皮祖细胞/干细胞群或角膜上皮细胞群; 以及用这些细胞群制备的用于治疗上皮疾病和细胞片的细胞制剂。

公开(公告)号：US09347041B2

公开(公告)日：2016-05-24

申请号：US14233056

申请日：2012-07-13

申请人： Ryuhei Hayashi ,  Susumu Hara ,  Tomofumi Kageyama ,  Kohji Nishida

发明人： Ryuhei Hayashi ,  Susumu Hara ,  Tomofumi Kageyama ,  Kohji Nishida

IPC分类号： C12N5/02 ,  C12N5/079 ,  A61K35/30

CPC分类号： C12N5/0621 ,  A61K35/30 ,  C12N2501/115 ,  C12N2501/48 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90

摘要： The present invention relates to a method for preparing corneal endothelial progenitor cells by adherent culturing a cell population isolated from corneal endothelial cell tissue at a low density by using a serum-free medium. The present invention also relates to a method for preparing corneal endothelial cells by differentiation-inducing the corneal endothelial progenitor cells obtained by the aforementioned method. According to the present invention, corneal endothelial progenitor cells can be selectively grown from a corneal tissue-derived cell population, and corneal endothelial cells obtained by inducing the corneal endothelial progenitor cells can be applied to treatment of corneal endothelial diseases. As a result, problems of corneal transplantation such as shortage of donors and occurrence of rejection can be solved.

摘要翻译： 本发明涉及通过使用无血清培养基以低密度粘附培养从角膜内皮细胞组织分离的细胞群来制备角膜内皮祖细胞的方法。 本发明还涉及通过分化诱导通过上述方法获得的角膜内皮祖细胞来制备角膜内皮细胞的方法。 根据本发明，可以从角膜组织衍生的细胞群中选择性地生长角膜内皮祖细胞，并且通过诱导角膜内皮祖细胞获得的角膜内皮细胞可用于治疗角膜内皮细胞疾病。 结果，可以解决诸如供体不足和排斥发生等角膜移植的问题。

公开(公告)号：US20140170751A1

公开(公告)日：2014-06-19

申请号：US14233056

申请日：2012-07-13

申请人： Ryuhei Hayashi ,  Susumu Hara ,  Tomofumi Kageyama ,  Kohji Nishida

发明人： Ryuhei Hayashi ,  Susumu Hara ,  Tomofumi Kageyama ,  Kohji Nishida

IPC分类号： C12N5/079

CPC分类号： C12N5/0621 ,  A61K35/30 ,  C12N2501/115 ,  C12N2501/48 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90

摘要： The present invention relates to a method for preparing corneal endothelial progenitor cells by adherent culturing a cell population isolated from corneal endothelial cell tissue at a low density by using a serum-free medium. The present invention also relates to a method for preparing corneal endothelial cells by differentiation-inducing the corneal endothelial progenitor cells obtained by the aforementioned method. According to the present invention, corneal endothelial progenitor cells can be selectively grown from a corneal tissue-derived cell population, and corneal endothelial cells obtained by inducing the corneal endothelial progenitor cells can be applied to treatment of corneal endothelial diseases. As a result, problems of corneal transplantation such as shortage of donors and occurrence of rejection can be solved.

摘要翻译： 本发明涉及通过使用无血清培养基以低密度粘附培养从角膜内皮细胞组织分离的细胞群来制备角膜内皮祖细胞的方法。 本发明还涉及通过分化诱导通过上述方法获得的角膜内皮祖细胞来制备角膜内皮细胞的方法。 根据本发明，可以从角膜组织衍生的细胞群中选择性地生长角膜内皮祖细胞，并且通过诱导角膜内皮祖细胞获得的角膜内皮细胞可用于治疗角膜内皮细胞疾病。 结果，可以解决诸如供体不足和排斥发生等角膜移植的问题。