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    Method for preparing corneal endothelial cell
    1.
    发明授权
    Method for preparing corneal endothelial cell 有权
    角膜内皮细胞制备方法

    公开(公告)号：US09347041B2

    公开(公告)日：2016-05-24

    申请号：US14233056

    申请日：2012-07-13

    申请人： Ryuhei Hayashi ,  Susumu Hara ,  Tomofumi Kageyama ,  Kohji Nishida

    发明人： Ryuhei Hayashi ,  Susumu Hara ,  Tomofumi Kageyama ,  Kohji Nishida

    IPC分类号： C12N5/02 ,  C12N5/079 ,  A61K35/30

    CPC分类号： C12N5/0621 ,  A61K35/30 ,  C12N2501/115 ,  C12N2501/48 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90

    摘要： The present invention relates to a method for preparing corneal endothelial progenitor cells by adherent culturing a cell population isolated from corneal endothelial cell tissue at a low density by using a serum-free medium. The present invention also relates to a method for preparing corneal endothelial cells by differentiation-inducing the corneal endothelial progenitor cells obtained by the aforementioned method. According to the present invention, corneal endothelial progenitor cells can be selectively grown from a corneal tissue-derived cell population, and corneal endothelial cells obtained by inducing the corneal endothelial progenitor cells can be applied to treatment of corneal endothelial diseases. As a result, problems of corneal transplantation such as shortage of donors and occurrence of rejection can be solved.

    摘要翻译： 本发明涉及通过使用无血清培养基以低密度粘附培养从角膜内皮细胞组织分离的细胞群来制备角膜内皮祖细胞的方法。 本发明还涉及通过分化诱导通过上述方法获得的角膜内皮祖细胞来制备角膜内皮细胞的方法。 根据本发明，可以从角膜组织衍生的细胞群中选择性地生长角膜内皮祖细胞，并且通过诱导角膜内皮祖细胞获得的角膜内皮细胞可用于治疗角膜内皮细胞疾病。 结果，可以解决诸如供体不足和排斥发生等角膜移植的问题。

    METHOD FOR PREPARING CORNEAL ENDOTHELIAL CELL
    2.
    发明申请
    METHOD FOR PREPARING CORNEAL ENDOTHELIAL CELL 有权
    制备角膜内皮细胞的方法

    公开(公告)号：US20140170751A1

    公开(公告)日：2014-06-19

    申请号：US14233056

    申请日：2012-07-13

    申请人： Ryuhei Hayashi ,  Susumu Hara ,  Tomofumi Kageyama ,  Kohji Nishida

    发明人： Ryuhei Hayashi ,  Susumu Hara ,  Tomofumi Kageyama ,  Kohji Nishida

    IPC分类号： C12N5/079

    CPC分类号： C12N5/0621 ,  A61K35/30 ,  C12N2501/115 ,  C12N2501/48 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90

    摘要： The present invention relates to a method for preparing corneal endothelial progenitor cells by adherent culturing a cell population isolated from corneal endothelial cell tissue at a low density by using a serum-free medium. The present invention also relates to a method for preparing corneal endothelial cells by differentiation-inducing the corneal endothelial progenitor cells obtained by the aforementioned method. According to the present invention, corneal endothelial progenitor cells can be selectively grown from a corneal tissue-derived cell population, and corneal endothelial cells obtained by inducing the corneal endothelial progenitor cells can be applied to treatment of corneal endothelial diseases. As a result, problems of corneal transplantation such as shortage of donors and occurrence of rejection can be solved.

    摘要翻译： 本发明涉及通过使用无血清培养基以低密度粘附培养从角膜内皮细胞组织分离的细胞群来制备角膜内皮祖细胞的方法。 本发明还涉及通过分化诱导通过上述方法获得的角膜内皮祖细胞来制备角膜内皮细胞的方法。 根据本发明，可以从角膜组织衍生的细胞群中选择性地生长角膜内皮祖细胞，并且通过诱导角膜内皮祖细胞获得的角膜内皮细胞可用于治疗角膜内皮细胞疾病。 结果，可以解决诸如供体不足和排斥发生等角膜移植的问题。

    iPS CELL HAVING DIFFERENTIATION PROPENSITY FOR CORNEAL EPITHELIUM
    5.
    发明申请
    iPS CELL HAVING DIFFERENTIATION PROPENSITY FOR CORNEAL EPITHELIUM 审中-公开
    iPS细胞具有对角膜上皮的差异性特征

    公开(公告)号：US20140127803A1

    公开(公告)日：2014-05-08

    申请号：US14112572

    申请日：2012-04-20

    申请人： Ryuhei Hayashi ,  Kohji Nishida

    发明人： Ryuhei Hayashi ,  Kohji Nishida

    IPC分类号： C12N5/071

    CPC分类号： C12N5/0602 ,  C12N5/0621 ,  C12N5/0623 ,  C12N5/0696 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2502/1394 ,  C12N2506/09 ,  C12N2506/45 ,  C12N2510/00

    摘要： A major object of the present invention is to provide a method for inducing cell differentiation into corneal epithelial stem cells and/or corneal epithelial cells, for the easy production of a corneal epithelial cell sheet having superior safety in view of the possibility of vascularization and the like occurring. A method for inducing differentiation of a pluripotent stem cell into a corneal epithelial stem cell and/or a corneal epithelial cell, the method comprising the step of culturing a pluripotent stem cell in the presence of a stromal cell or an amnion-derived factor.

    摘要翻译： 本发明的主要目的是提供一种诱导细胞分化成角膜上皮干细胞和/或角膜上皮细胞的方法，从容易地生产角膜上皮细胞片，鉴于血管形成的可能性而具有优异的安全性 喜欢发生。 一种用于诱导多能干细胞分化成角膜上皮干细胞和/或角膜上皮细胞的方法，所述方法包括在基质细胞或羊膜衍生因子存在下培养多能干细胞的步骤。