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    Method for preparing corneal endothelial cell
    2.
    发明授权
    Method for preparing corneal endothelial cell 有权
    角膜内皮细胞制备方法

    公开(公告)号：US09347041B2

    公开(公告)日：2016-05-24

    申请号：US14233056

    申请日：2012-07-13

    申请人： Ryuhei Hayashi ,  Susumu Hara ,  Tomofumi Kageyama ,  Kohji Nishida

    发明人： Ryuhei Hayashi ,  Susumu Hara ,  Tomofumi Kageyama ,  Kohji Nishida

    IPC分类号： C12N5/02 ,  C12N5/079 ,  A61K35/30

    CPC分类号： C12N5/0621 ,  A61K35/30 ,  C12N2501/115 ,  C12N2501/48 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90

    摘要： The present invention relates to a method for preparing corneal endothelial progenitor cells by adherent culturing a cell population isolated from corneal endothelial cell tissue at a low density by using a serum-free medium. The present invention also relates to a method for preparing corneal endothelial cells by differentiation-inducing the corneal endothelial progenitor cells obtained by the aforementioned method. According to the present invention, corneal endothelial progenitor cells can be selectively grown from a corneal tissue-derived cell population, and corneal endothelial cells obtained by inducing the corneal endothelial progenitor cells can be applied to treatment of corneal endothelial diseases. As a result, problems of corneal transplantation such as shortage of donors and occurrence of rejection can be solved.

    摘要翻译： 本发明涉及通过使用无血清培养基以低密度粘附培养从角膜内皮细胞组织分离的细胞群来制备角膜内皮祖细胞的方法。 本发明还涉及通过分化诱导通过上述方法获得的角膜内皮祖细胞来制备角膜内皮细胞的方法。 根据本发明，可以从角膜组织衍生的细胞群中选择性地生长角膜内皮祖细胞，并且通过诱导角膜内皮祖细胞获得的角膜内皮细胞可用于治疗角膜内皮细胞疾病。 结果，可以解决诸如供体不足和排斥发生等角膜移植的问题。

    METHOD FOR PREPARING CORNEAL ENDOTHELIAL CELL
    3.
    发明申请
    METHOD FOR PREPARING CORNEAL ENDOTHELIAL CELL 有权
    制备角膜内皮细胞的方法

    公开(公告)号：US20140170751A1

    公开(公告)日：2014-06-19

    申请号：US14233056

    申请日：2012-07-13

    申请人： Ryuhei Hayashi ,  Susumu Hara ,  Tomofumi Kageyama ,  Kohji Nishida

    发明人： Ryuhei Hayashi ,  Susumu Hara ,  Tomofumi Kageyama ,  Kohji Nishida

    IPC分类号： C12N5/079

    CPC分类号： C12N5/0621 ,  A61K35/30 ,  C12N2501/115 ,  C12N2501/48 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/90

    摘要： The present invention relates to a method for preparing corneal endothelial progenitor cells by adherent culturing a cell population isolated from corneal endothelial cell tissue at a low density by using a serum-free medium. The present invention also relates to a method for preparing corneal endothelial cells by differentiation-inducing the corneal endothelial progenitor cells obtained by the aforementioned method. According to the present invention, corneal endothelial progenitor cells can be selectively grown from a corneal tissue-derived cell population, and corneal endothelial cells obtained by inducing the corneal endothelial progenitor cells can be applied to treatment of corneal endothelial diseases. As a result, problems of corneal transplantation such as shortage of donors and occurrence of rejection can be solved.

    摘要翻译： 本发明涉及通过使用无血清培养基以低密度粘附培养从角膜内皮细胞组织分离的细胞群来制备角膜内皮祖细胞的方法。 本发明还涉及通过分化诱导通过上述方法获得的角膜内皮祖细胞来制备角膜内皮细胞的方法。 根据本发明，可以从角膜组织衍生的细胞群中选择性地生长角膜内皮祖细胞，并且通过诱导角膜内皮祖细胞获得的角膜内皮细胞可用于治疗角膜内皮细胞疾病。 结果，可以解决诸如供体不足和排斥发生等角膜移植的问题。

    iPS CELL HAVING DIFFERENTIATION PROPENSITY FOR CORNEAL EPITHELIUM
    5.
    发明申请
    iPS CELL HAVING DIFFERENTIATION PROPENSITY FOR CORNEAL EPITHELIUM 审中-公开
    iPS细胞具有对角膜上皮的差异性特征

    公开(公告)号：US20140127803A1

    公开(公告)日：2014-05-08

    申请号：US14112572

    申请日：2012-04-20

    申请人： Ryuhei Hayashi ,  Kohji Nishida

    发明人： Ryuhei Hayashi ,  Kohji Nishida

    IPC分类号： C12N5/071

    CPC分类号： C12N5/0602 ,  C12N5/0621 ,  C12N5/0623 ,  C12N5/0696 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/606 ,  C12N2502/1394 ,  C12N2506/09 ,  C12N2506/45 ,  C12N2510/00

    摘要： A major object of the present invention is to provide a method for inducing cell differentiation into corneal epithelial stem cells and/or corneal epithelial cells, for the easy production of a corneal epithelial cell sheet having superior safety in view of the possibility of vascularization and the like occurring. A method for inducing differentiation of a pluripotent stem cell into a corneal epithelial stem cell and/or a corneal epithelial cell, the method comprising the step of culturing a pluripotent stem cell in the presence of a stromal cell or an amnion-derived factor.

    摘要翻译： 本发明的主要目的是提供一种诱导细胞分化成角膜上皮干细胞和/或角膜上皮细胞的方法，从容易地生产角膜上皮细胞片，鉴于血管形成的可能性而具有优异的安全性 喜欢发生。 一种用于诱导多能干细胞分化成角膜上皮干细胞和/或角膜上皮细胞的方法，所述方法包括在基质细胞或羊膜衍生因子存在下培养多能干细胞的步骤。

    Method of treatment using corneal epithelium forming cell sheets
    8.
    发明授权
    Method of treatment using corneal epithelium forming cell sheets 有权

    公开(公告)号：US10434219B2

    公开(公告)日：2019-10-08

    申请号：US15859304

    申请日：2017-12-29

    申请人： CellSeed Inc. ,  Kohji Nishida

    发明人： Teruo Okano ,  Kohji Nishida ,  Masayuki Yamato

    IPC分类号： A61L27/38 ,  C12N5/079

    摘要： A diseased site where an anterior segment tissue is partly or entirely damaged or deficient can be treated using a corneal epithelium forming cell sheet that will adhere well to the anterior segment tissue. To attain this objective, a corneal epithelium forming cell sheet is produced by a process comprising the steps of cultivating under specified conditions corneal epithelium forming cells on a cell culture support comprising a substrate having its surface covered with a temperature responsive polymer of which the hydrating force varies in a temperature range of 0° C.-80° C., optionally stratifying the layer of cultured cells, and thereafter, (1) adjusting the temperature of the culture solution to either above an upper critical dissolution temperature or below a lower critical dissolution temperature, (2) bringing the cultured corneal epithelium forming cells into close contact with a carrier, and (3) detaching the sheet together with the carrier under specified conditions.

    Noncovalent collagen crosslinking agent
    9.
    发明授权
    Noncovalent collagen crosslinking agent 失效
    非共价胶原交联剂

    公开(公告)号：US08389686B2

    公开(公告)日：2013-03-05

    申请号：US12990897

    申请日：2009-05-01

    申请人： Mitsuru Akashi ,  Kohji Nishida ,  Michiya Matsusaki ,  Akira Kubota ,  Tomonori Waku

    发明人： Mitsuru Akashi ,  Kohji Nishida ,  Michiya Matsusaki ,  Akira Kubota ,  Tomonori Waku

    IPC分类号： C07K14/78 ,  A61K38/00

    CPC分类号： A61K38/39

    摘要： The present invention provides a collagen crosslinking agent superior in biocompatibility that is free from the damage by UV irradiation and also from the problems of toxicity caused by residual monomer or unreacted functional groups. Provided is a noncovalent collagen crosslinking agent (for fibrous protein collagen), comprising a spacer of a polyvalent alcohol having two or more OH groups at the terminals and arms of collagen peptides formed of repetitions of three amino acids, the arms being bound via the OH groups to the spacer.

    摘要翻译： 本发明提供一种无生物相容性优异的胶原交联剂，其不受紫外线照射的损伤，也可由残留单体或未反应官能团引起的毒性问题。 提供了一种非共价胶原交联剂（纤维蛋白胶原蛋白），其包含在末端具有两个或更多个OH基团的多元醇的隔离物和由三个氨基酸重复形成的胶原肽的臂，所述臂通过OH结合 组到间隔物。