公开(公告)号：US07759072B2

公开(公告)日：2010-07-20

申请号：US11817515

申请日：2006-03-02

申请人： Koji Ushizawa ,  Michiko Kawamoto ,  Shoko Yamamoto ,  Kumiko Yuki ,  Yoko Ikeda ,  Mitsuaki Yamamoto

发明人： Koji Ushizawa ,  Michiko Kawamoto ,  Shoko Yamamoto ,  Kumiko Yuki ,  Yoko Ikeda ,  Mitsuaki Yamamoto

IPC分类号： G01N33/53

CPC分类号： G01N33/581 ,  G01N33/5306 ,  G01N33/542 ,  G01N33/54393

摘要： The present invention provides a novel immunoassay method with high reaction specificity and high sensitivity. The present invention also provides a method for immunoassaying a target antigen utilizing reactivation of an apoenzyme, which includes simultaneously or sequentially adding a test sample to an antibody specific to the target antigen, the target antigen labeled with a coenzyme, an apo-D-amino acid oxidase, a D-amino acid, and a reagent for detecting a hydrogen peroxide produced by the oxidase.

摘要翻译： 本发明提供了具有高反应特异性和高灵敏度的新型免疫测定方法。 本发明还提供了使用辅酶的再活化来免疫测定靶抗原的方法，其包括同时或依次将测试样品加入到靶抗原特异性抗体中，用辅酶标记的靶抗原，载脂蛋白-D-氨基 酸性氧化酶，D-氨基酸和用于检测由氧化酶产生的过氧化氢的试剂。

公开(公告)号：US20090035785A1

公开(公告)日：2009-02-05

申请号：US11817515

申请日：2006-03-02

申请人： Koji Ushizawa ,  Michiko Kawamoto ,  Shoko Yamamoto ,  Kumiko Yuki ,  Yoko Ikeda ,  Mitsuaki Yamamoto

发明人： Koji Ushizawa ,  Michiko Kawamoto ,  Shoko Yamamoto ,  Kumiko Yuki ,  Yoko Ikeda ,  Mitsuaki Yamamoto

IPC分类号： G01N33/53

CPC分类号： G01N33/581 ,  G01N33/5306 ,  G01N33/542 ,  G01N33/54393

摘要： The present invention provides a novel immunoassay method with high reaction specificity and high sensitivity. The present invention also provides a method for immunoassaying a target antigen utilizing reactivation of an apoenzyme, which includes simultaneously or sequentially adding a test sample to an antibody specific to the target antigen, the target antigen labeled with a coenzyme, an apo-D-amino acid oxidase, a D-amino acid, and a reagent for detecting a hydrogen peroxide produced by the oxidase.

摘要翻译： 本发明提供了具有高反应特异性和高灵敏度的新型免疫测定方法。 本发明还提供了使用辅酶的再活化来免疫测定靶抗原的方法，其包括同时或依次将测试样品加入到靶抗原特异性抗体中，用辅酶标记的靶抗原，载脂蛋白-D-氨基 酸性氧化酶，D-氨基酸和用于检测由氧化酶产生的过氧化氢的试剂。

公开(公告)号：US20070015141A1

公开(公告)日：2007-01-18

申请号：US11483883

申请日：2006-07-10

申请人： Sachiko Ito ,  Michiko Kawamoto ,  Kiichiro Kobori ,  Koji Ushizawa

发明人： Sachiko Ito ,  Michiko Kawamoto ,  Kiichiro Kobori ,  Koji Ushizawa

IPC分类号： C12Q1/70 ,  G01N33/553

CPC分类号： G01N15/0205 ,  G01N33/585

摘要： A method for accurately determining the particle diameter of gold colloid in a simple manner, and a gold colloid solution with a particle diameter within a desired range are described. A method for estimating the particle diameter distribution width of gold colloid based on the measurement of, in addition to the maximum absorption wavelength, a ratio (AλX/Aλmax) of the absorbance (AλX) at a wavelength differing from the maximum absorption wavelength to the absorbance (Aλmax) at the maximum absorption wavelength of the gold colloid solution, and the gold colloid solution obtained by the method are provided.

摘要翻译： 描述了以简单的方式精确测定金胶体的粒径的方法和粒径在期望范围内的金胶体溶液。 基于除了最大吸收波长之外的测量，用于估计金胶体的粒径分布宽度的方法，其比例（λmax / 在金胶体溶液的最大吸收波长处，与最大吸收波长不同于吸光度（λmax max）的波长处的吸光度（Al x X S）和金 提供了通过该方法获得的胶体溶液。

公开(公告)号：US20070015140A1

公开(公告)日：2007-01-18

申请号：US10546691

申请日：2004-03-09

申请人： Kiichiro Kobori ,  Michiko Kawamoto ,  Koji Ushizawa

发明人： Kiichiro Kobori ,  Michiko Kawamoto ,  Koji Ushizawa

IPC分类号： C12Q1/70 ,  C07K16/10

CPC分类号： B01L3/50825 ,  B01L2300/046 ,  B01L2300/0681 ,  G01N33/5304 ,  G01N2333/11

摘要： A method of immunologically examining a specimen which comprises attaching a cap having a filter impregnated with a labeled antibody enclosed therein to a container body containing a diluted liquid specimen, pouring the diluted liquid specimen from the container into a test device, observing the reaction and thus examining the presence or absence of an analyte in the specimen. This examination method, whereby effects of differences among individual operators can be minimized and the occurrence of a nonspecific reaction can be prevented, is highly excellent in the reproducibility of the examination results and storage stability of a test reagent. A specimen container to be used in the above method to which a cap having a filter impregnated with a labeled antibody enclosed therein is attached. This container is appropriately usable as a member constituting a simplified diagnosis kit.

摘要翻译： 一种免疫检查样品的方法，其包括将包含标记抗体的过滤器的盖子封装在包含稀释液体样品的容器体中，将稀释的液体样品从容器中倒入测试装置中，观察反应，从而 检查样品中分析物的存在或不存在。 这种检查方法能够将各个操作者之间的差异的效果最小化并且可以防止非特异性反应的发生，检查结果的再现性和试验试剂的储存稳定性都非常优异。 在上述方法中使用的标本容器，其中附着有浸渍有标记抗体的过滤器的盖。 该容器适合用作构成简化诊断试剂盒的部件。

公开(公告)号：US07442498B2

公开(公告)日：2008-10-28

申请号：US10546691

申请日：2004-03-09

申请人： Kiichiro Kobori ,  Michiko Kawamoto ,  Koji Ushizawa

发明人： Kiichiro Kobori ,  Michiko Kawamoto ,  Koji Ushizawa

IPC分类号： C12Q1/70 ,  C12Q1/00 ,  G01N55/543 ,  G01N55/553 ,  G01N31/00

CPC分类号： B01L3/50825 ,  B01L2300/046 ,  B01L2300/0681 ,  G01N33/5304 ,  G01N2333/11

摘要： A method of immunologically examining a specimen which comprises attaching a cap having a filter impregnated with a labeled antibody enclosed therein to a container body containing a diluted liquid specimen, pouring the diluted liquid specimen from the container into a test device, observing the reaction and thus examining the presence or absence of an analyte in the specimen. This examination method, whereby effects of differences among individual operators can be minimized and the occurrence of a nonspecific reaction can be prevented, is highly excellent in the reproducibility of the examination results and storage stability of a test reagent. A specimen container to be used in the above method to which a cap having a filter impregnated with a labeled antibody enclosed therein is attached. This container is appropriately usable as a member constituting a simplified diagnosis kit.

摘要翻译： 一种免疫检查样品的方法，其包括将包含标记抗体的过滤器的盖子封装在包含稀释液体样品的容器体中，将稀释的液体样品从容器中倒入测试装置中，观察反应，从而 检查样品中分析物的存在或不存在。 这种检查方法能够将各个操作者之间的差异的效果最小化并且可以防止非特异性反应的发生，检查结果的再现性和试验试剂的储存稳定性都非常优异。 在上述方法中使用的标本容器，其中附着有浸渍有标记抗体的过滤器的盖。 该容器适合用作构成简化诊断试剂盒的部件。

公开(公告)号：US20140349284A1

公开(公告)日：2014-11-27

申请号：US14008770

申请日：2012-03-30

申请人： Takuya Yotani ,  Eiji Kiyotoh ,  Koji Ushizawa

发明人： Takuya Yotani ,  Eiji Kiyotoh ,  Koji Ushizawa

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6881 ,  C12Q1/6858 ,  C12Q2565/137

摘要： An object of the present invention is to provide a sample nucleic acid for single nucleotide polymorphism detection which is for use in a simple method for quickly detecting a single nucleotide polymorphism, PCR primers for preparation of a sample for single nucleotide polymorphism detection, and a sample for single nucleotide polymorphism detection which is for use in ion exchange chromatography analysis. The present invention provides a sample nucleic acid for single nucleotide polymorphism detection having the following features: (a) a full chain length of 200 bp or less; (b) a sequence (a tag sequence) incompletely complementary to a template DNA at the 5′ or 3′ end; and (c) a 10 bp or less difference in chain length from a sample nucleic acid to be compared with to determine the presence of a single nucleotide polymorphism.

摘要翻译： 本发明的目的是提供用于单核苷酸多态性快速检测的简单方法的单核苷酸多态性检测用样品核酸，用于制备单核苷酸多态性检测用样品的PCR引物和样品 用于单核苷酸多态性检测，用于离子交换色谱分析。 本发明提供了用于单核苷酸多态性检测的样品核酸，其具有以下特征：（a）200bp或更短的完整链长度; （b）在5'或3'末端与模板DNA不完全互补的序列（标签序列） 和（c）与要比较的样品核酸的链长的差异为10bp或更小，以确定单核苷酸多态性的存在。

公开(公告)号：US07198890B2

公开(公告)日：2007-04-03

申请号：US10755236

申请日：2004-01-13

申请人： Hiroyuki Ebinuma ,  Takami Sarashina ,  Takuji Matsumoto ,  Masahiro Sekiguchi ,  Koji Ushizawa

发明人： Hiroyuki Ebinuma ,  Takami Sarashina ,  Takuji Matsumoto ,  Masahiro Sekiguchi ,  Koji Ushizawa

IPC分类号： C12Q1/00 ,  C12N9/00 ,  C07K1/00

CPC分类号： G01N33/6812 ,  G01N2333/90241

摘要： The present invention provides a method for quantitatively determining homocysteine in a biological specimen containing homocysteine and cysteine by use of an enzyme which is capable of forming hydrogen sulfide both from homocysteine and from cysteine, which comprises (a) reacting the biological specimen with cysteine dioxygenase in the absence of a reducing agent, (b) subsequently reacting the resultant specimen of (a) with a reducing agent and the enzyme which is capable of forming hydrogen sulfide both from homocysteine and from cysteine, and (c) measuring the concentration of the hydrogen sulfide thus obtained to determine the homocysteine concentration in the biological specimen; and a reagent for such a quantitative determination of homocysteine.

摘要翻译： 本发明提供了一种通过使用能够从高半胱氨酸和半胱氨酸形成硫化氢的酶来定量测定含有同型半胱氨酸和半胱氨酸的生物样品中的同型半胱氨酸的方法，其包括（a）使生物样品与半胱氨酸双加氧酶在 不存在还原剂，（b）随后使（a）的所得样品与还原剂和能够从高半胱氨酸和半胱氨酸形成硫化氢的酶反应，和（c）测量氢的浓度 硫化物，以确定生物样品中的同型半胱氨酸浓度; 和用于定量测定同型半胱氨酸的试剂。

公开(公告)号：US20050153385A1

公开(公告)日：2005-07-14

申请号：US10755236

申请日：2004-01-13

申请人： Hiroyuki Ebinuma ,  Takami Sarashina ,  Takuji Matsumoto ,  Masahiro Sekiguchi ,  Koji Ushizawa

发明人： Hiroyuki Ebinuma ,  Takami Sarashina ,  Takuji Matsumoto ,  Masahiro Sekiguchi ,  Koji Ushizawa

IPC分类号： C12Q1/26 ,  C12Q1/527 ,  G01N33/53 ,  G01N33/68

CPC分类号： G01N33/6812 ,  G01N2333/90241

摘要： The present invention provides a method for quantitatively determining homocysteine in a biological specimen containing homocysteine and cysteine by use of an enzyme which is capable of forming hydrogen sulfide both from homocysteine and from cysteine, which comprises (a) reacting the biological specimen with cysteine dioxygenase in the absence of a reducing agent, (b) subsequently reacting the resultant specimen of (a) with a reducing agent and the enzyme which is capable of forming hydrogen sulfide both from homocysteine and from cysteine, and (c) measuring the concentration of the hydrogen sulfide thus obtained to determine the homocysteine concentration in the biological specimen; and a reagent for such a quantitative determination of homocysteine.

摘要翻译： 本发明提供了一种通过使用能够从高半胱氨酸和半胱氨酸形成硫化氢的酶来定量测定含有同型半胱氨酸和半胱氨酸的生物样品中的同型半胱氨酸的方法，其包括（a）使生物样品与半胱氨酸双加氧酶在 不存在还原剂，（b）随后使（a）的所得样品与还原剂和能够从高半胱氨酸和半胱氨酸形成硫化氢的酶反应，和（c）测量氢的浓度 硫化物，以确定生物样品中的同型半胱氨酸浓度; 和用于定量测定同型半胱氨酸的试剂。

公开(公告)号：US20140030713A1

公开(公告)日：2014-01-30

申请号：US13984107

申请日：2012-02-09

申请人： Takuya Yotani ,  Koji Ushizawa

发明人： Takuya Yotani ,  Koji Ushizawa

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6827 ,  B01D15/363 ,  B01J41/20 ,  C12N15/1006 ,  G01N30/96 ,  G01N2030/8827 ,  B01J41/05 ,  B01J41/07

摘要： The present invention aims to provide a filler for ion exchange chromatography which can sufficiently detect nucleic acid chains that differ in sequence of bases or nucleic acid chains that differ by a single base substitution. The present invention also aims to provide a method for separating and detecting a nucleic acid chain using the filler for ion exchange chromatography. The present invention relates to filler for ion exchange chromatography, comprising base fine particles, each particle having a strong cationic group and a weak cationic group on the surface thereof.

摘要翻译： 本发明的目的是提供一种用于离子交换色谱的填充剂，其可以充分地检测由单碱基取代而不同的碱基或核酸链序列不同的核酸链。 本发明还旨在提供一种使用离子交换层析用填料分离检测核酸链的方法。 本发明涉及用于离子交换层析的填料，其包括基础细颗粒，其表面上具有强阳离子基团和弱阳离子基团的每个颗粒。

公开(公告)号：US20070026523A1

公开(公告)日：2007-02-01

申请号：US11492082

申请日：2006-07-25

申请人： Yuriko Taniguchi ,  Tomohisa Nishio ,  Koji Ushizawa

发明人： Yuriko Taniguchi ,  Tomohisa Nishio ,  Koji Ushizawa

IPC分类号： G01N31/00

CPC分类号： C12Q1/37 ,  G01N33/52 ,  G01N33/721 ,  Y10T436/108331

摘要： Provided is a method for stabilizing a leuco dye, the method including storing a leuco dye in a solution in the co-presence of a protease protein.

摘要翻译： 提供了一种稳定无色染料的方法，该方法包括将无色染料存储在蛋白酶蛋白共存中的溶液中。