公开(公告)号：US10760051B2

公开(公告)日：2020-09-01

申请号：US15506626

申请日：2015-08-13

Applicant: Korea Research Institute of Bioscience and Biotechnology

Inventor： Baek Soo Han ,  Sang Chul Lee ,  Kwang-Hee Bae ,  Cheonok Lee ,  Won Kon Kim ,  Kyoung Jin Oh

IPC: C12N5/079

Abstract: The present invention relates to a method for producing astrocytes comprising obtaining neural progenitor cells from stem cells so as to continuously produce astrocytes with high purity and same traits, followed by two steps of differentiating the neural progenitor cells into the astrocytes, and astrocytes produced therefrom. Since the method of preparing the astrocytes provided in the present invention enables not only production of the astrocytes with high purity and faster production of the astrocytes with same characteristics, but also rapid differentiation of the astrocytes using the neural progenitor cells when necessary, it can be widely used for effectively treating a patient with a disease which requires transplantation of the astrocytes.

公开(公告)号：US10231999B2

公开(公告)日：2019-03-19

申请号：US15506216

申请日：2015-08-13

Applicant: Korea Research Institute of Bioscience and Biotechnology

Inventor： Sang Chul Lee ,  Baek Soo Han ,  Kwang-Hee Bae ,  Hyejin Kim ,  Won Kon Kim ,  Kyoung Jin Oh

IPC: A61K35/30 ,  C12N5/0793 ,  C12N5/10 ,  A61K38/17 ,  C12N5/074 ,  A61K45/00 ,  A61K48/00 ,  C12N5/00

Abstract: The present invention relates to a method for producing cholinergic neurons comprising obtaining neural progenitor cells from stem cells so as to continuously produce cholinergic neural cells with high purity and the same traits, followed by differentiating the neural progenitor cells into the cholinergic neurons, and cholinergic neurons produced therefrom. Since the method of preparing the cholinergic neurons provided in the present invention enables not only production of the cholinergic neurons with high purity, but also rapid production of the cholinergic neurons with the same traits, it can be widely used for effectively treating degenerative cranial nerve diseases such as Alzheimer's disease.

公开(公告)号：US09752190B2

公开(公告)日：2017-09-05

申请号：US14806514

申请日：2015-07-22

Applicant: Korea Research Institute of Bioscience and Biotechnology

Inventor： Jeong Ki Min ,  Jongjin Park ,  Kwang-Hee Bae ,  Sang Chul Lee ,  Yee Sook Cho

IPC: C07K16/28 ,  C12Q1/00 ,  G01N33/50 ,  C12Q1/68 ,  G01N33/569

CPC classification number: C12Q1/6881 ,  C07K16/28 ,  C12Q2600/158 ,  G01N33/56966 ,  G01N2333/705 ,  G01N2400/00

Abstract: The present invention relates to a composition for detecting the undifferentiated human pluripotent stem cells comprising an agent useful for measuring the level of Desmoglein 2 (Dsg 2) mRNA or the protein thereof, a kit for detecting the undifferentiated human pluripotent stem cells comprising the said composition, a method for detecting the undifferentiated human pluripotent stem cells containing the step of measuring the level of Desmoglein 2 mRNA or the protein thereof, a method for evaluating the differentiation of human pluripotent stem cells and thereafter for separating the undifferentiated human pluripotent stem cells, a method for reducing the undifferentiated status of human pluripotent stem cells by inhibiting the expression or activation of Desmoglein 2, and a monoclonal antibody binding specifically to human Desmoglein 2.

公开(公告)号：US20170267971A1

公开(公告)日：2017-09-21

申请号：US15506626

申请日：2015-08-13

Applicant: Korea Research Institute of Bioscience and Biotechnology

Inventor： Baek Soo Han ,  Sang Chul Lee ,  Kwang-Hee Bae ,  Cheonok Lee ,  Won Kon Kim ,  Kyoung Jin Oh

IPC: C12N5/079

Abstract: The present invention relates to a method for producing astrocytes comprising obtaining neural progenitor cells from stem cells so as to continuously produce astrocytes with high purity and same traits, followed by two steps of differentiating the neural progenitor cells into the astrocytes, and astrocytes produced therefrom. Since the method of preparing the astrocytes provided in the present invention enables not only production of the astrocytes with high purity and faster production of the astrocytes with same characteristics, but also rapid differentiation of the astrocytes using the neural progenitor cells when necessary, it can be widely used for effectively treating a patient with a disease which requires transplantation of the astrocytes.

公开(公告)号：US20170266235A1

公开(公告)日：2017-09-21

申请号：US15506216

申请日：2015-08-13

Applicant: Korea Research Institute of Bioscience and Biotechnology

Inventor： Sang Chul Lee ,  Baek Soo Han ,  Kwang-Hee Bae ,  Hyejin Kim ,  Won Kon Kim ,  Kyoung Jin Oh

IPC: A61K35/30 ,  C12N5/0793 ,  C12N5/10 ,  A61K38/17 ,  C12N5/074

CPC classification number: A61K35/30 ,  A61K38/1787 ,  A61K45/00 ,  A61K48/00 ,  C12N5/00 ,  C12N5/0607 ,  C12N5/0619 ,  C12N5/10 ,  C12N2502/08

Abstract: The present invention relates to a method for producing cholinergic neurons comprising obtaining neural progenitor cells from stem cells so as to continuously produce cholinergic neural cells with high purity and the same traits, followed by differentiating the neural progenitor cells into the cholinergic neurons, and cholinergic neurons produced therefrom. Since the method of preparing the cholinergic neurons provided in the present invention enables not only production of the cholinergic neurons with high purity, but also rapid production of the cholinergic neurons with the same traits, it can be widely used for effectively treating degenerative cranial nerve diseases such as Alzheimer's disease.