公开(公告)号：US20070037243A1

公开(公告)日：2007-02-15

申请号：US10572052

申请日：2004-09-13

申请人： Kozo Hirokawa ,  Keiko Kurosawa ,  Naoki Kajiyama

发明人： Kozo Hirokawa ,  Keiko Kurosawa ,  Naoki Kajiyama

IPC分类号： C12Q1/26 ,  C12P21/06

CPC分类号： C12P21/02 ,  C12P21/06

摘要： The present invention relates to a method for producing α-glycated dipeptide, which comprises causing protease to act on N-terminal-glycated peptide or N-terminal-glycated protein. The present invention further relates to a method for determining the amount of α-glycated dipeptide, which comprises causing a fructosyl peptide oxidase to act on the α-glycated dipeptide obtained by the above method and then determining the amount of the thus generated hydrogen peroxide. According to the present invention, a method for producing α-glycated dipeptide is provided, which enables the simple, rapid, and efficient production of α-glycated dipeptide from glycated protein or glycated peptide. Furthermore, according to the present invention, a method for determining the amount of α-glycated dipeptide is provided, which enables to determine the amount of α-glycated dipeptide in a highly precise manner within a short time period.

摘要翻译： 本发明涉及α-糖基化二肽的制备方法，其包括使蛋白酶作用于N-末端糖基化肽或N-末端糖基化蛋白质。 本发明还涉及确定α-糖化二肽量的方法，其包括使果糖基肽氧化酶作用于通过上述方法获得的α-糖化二肽，然后测定由此产生的过氧化氢的量。 根据本发明，提供了α-糖化二肽的制备方法，能够从糖化蛋白质或糖化肽中简单，快速，有效地生产α-糖化二肽。 此外，根据本发明，提供了用于测定α-糖化二肽量的方法，其能够在短时间内以高度精确的方式测定α-糖化二肽的量。

公开(公告)号：US20090011467A1

公开(公告)日：2009-01-08

申请号：US12180780

申请日：2008-07-28

申请人： Keiko Kurosawa ,  Kozo Hirokawa ,  Naoki Kajiyama

发明人： Keiko Kurosawa ,  Kozo Hirokawa ,  Naoki Kajiyama

IPC分类号： C12P21/02 ,  C12N9/06 ,  C07H21/04 ,  C12N15/63 ,  C12N1/15

CPC分类号： C12N9/0022

摘要： The present invention has an object of providing a novel fructosyl peptide oxidase having superior physicochemical properties such as stability that is useful as an enzyme for clinical diagnosis, and an object of providing a method for producing the fructosyl peptide oxidase.A novel fructosyl peptide oxidase having physicochemical properties useful as an enzyme for clinical diagnosis, and a method for producing a novel fructosyl peptide oxidase are provided herein, the method comprising: culturing a microorganism capable of producing the oxidase in a medium; and collecting the oxidase from the culture. Furthermore, a fructosyl peptide oxidase gene coding for a novel fructosyl peptide oxidase, recombinant DNA wherein the gene is inserted into vector DNA, and a method for producing a novel fructosyl peptide oxidase are provided herein, the method comprising: culturing, in a medium, a transformant or a transductant including the gene; and collecting the novel fructosyl peptide oxidase from the culture.

摘要翻译： 本发明的目的是提供具有优异的物理化学性质的新型果糖基氧化酶，例如可用作临床诊断用酶的稳定性，以及提供生产果糖基氧化酶的方法的目的。 本发明提供了具有用作临床诊断用酶的物理化学特性的新型果糖基氧化酶，以及生产新型果糖基氧化酶的方法，该方法包括：培养能够在培养基中产生氧化酶的微生物; 并从培养物中收集氧化酶。 此外，本发明提供了编码新型果糖基肽氧化酶的果糖基肽氧化酶基因，其中将基因插入载体DNA中的重组DNA，以及生产新型果糖基氧化酶的方法，该方法包括：在培养基中培养， 包含该基因的转化体或转导体; 并从培养物中收集新颖的果糖基氧化酶。

公开(公告)号：US07049113B2

公开(公告)日：2006-05-23

申请号：US10343002

申请日：2001-07-26

申请人： Kozo Hirokawa ,  Keiko Kurosawa ,  Naoki Kajiyama

发明人： Kozo Hirokawa ,  Keiko Kurosawa ,  Naoki Kajiyama

IPC分类号： C12N15/53 ,  C12N9/02

CPC分类号： C12N9/0004 ,  C07K14/43563

摘要： The present invention relates to: an isolated or synthesized gene, which encodes a protein comprising the amino acid sequence represented by SEQ ID NO: 2, an isolated or synthesized gene, which encodes a protein comprising an amino acid sequence comprising a deletion, substitution or addition of one or more amino acids with respect to the amino acid sequence represented by SEQ ID NO: 2 and being capable of regenerating luciferin, an isolated or synthesized gene, which hybridizes with the complementary strand sequence of a DNA comprising the nucleotide sequence represented by SEQ ID NO: 1 under stringent conditions and encodes a protein capable of regenerating luciferin, a recombinant DNA, which is characterized in that the above-described isolated or synthesized gene is inserted into a vector DNA, a transformant or transductant comprising the above-described recombinant DNA, and a process for producing a protein capable of regenerating luciferin, which is characterized in that the method comprises culturing the above-described transformant or transductant in a medium and collecting therefrom the protein capable of regenerating luciferin.

摘要翻译： 本发明涉及：分离或合成的基因，其编码包含SEQ ID NO：2所示的氨基酸序列的蛋白质，分离或合成的基因，其编码包含缺失，取代或取代的氨基酸序列的蛋白质 相对于SEQ ID NO：2所示的氨基酸序列添加一个或多个氨基酸，并且能够再生荧光素，一种分离或合成的基因，其与包含以下的核苷酸序列的DNA的互补链序列杂交： SEQ ID NO：1，并编码能够再生荧光素的蛋白质，重组DNA，其特征在于将上述分离或合成的基因插入载体DNA，转化体或包含上述的转导体的转导体 重组DNA，以及能够再生荧光素的蛋白质的制造方法，其特征在于， od包括在培养基中培养上述转化体或转导体，并从中收集能够再生萤光素的蛋白质。

公开(公告)号：US07018823B2

公开(公告)日：2006-03-28

申请号：US10232655

申请日：2002-09-03

申请人： Keiko Kurosawa ,  Kozo Hirokawa ,  Naoki Kajiyama

发明人： Keiko Kurosawa ,  Kozo Hirokawa ,  Naoki Kajiyama

IPC分类号： C12N9/02 ,  C12N15/00 ,  C12N1/20 ,  C12Q1/26 ,  C07H21/04

CPC分类号： C12N9/0022

摘要： The present invention has an object of providing a novel fructosyl peptide oxidase having superior physicochemical properties such as stability that is useful as an enzyme for clinical diagnosis, and an object of providing a method for producing the fructosyl peptide oxidase.A novel fructosyl peptide oxidase having physicochemical properties useful as an enzyme for clinical diagnosis, and a method for producing a novel fructosyl peptide oxidase are provided herein, the method comprising: culturing a microorganism capable of producing the oxidase in a medium; and collecting the oxidase from the culture. Furthermore, a fructosyl peptide oxidase gene coding for a novel fructosyl peptide oxidase, recombinant DNA wherein the gene is inserted into vector DNA, and a method for producing a novel fructosyl peptide oxidase are provided herein, the method comprising: culturing, in a medium, a transformant or a transductant including the gene; and collecting the novel fructosyl peptide oxidase from the culture.

摘要翻译： 本发明的目的是提供具有优异的物理化学性质的新型果糖基氧化酶，例如可用作临床诊断用酶的稳定性，以及提供生产果糖基氧化酶的方法的目的。 本发明提供了具有用作临床诊断用酶的物理化学特性的新型果糖基氧化酶，以及生产新型果糖基氧化酶的方法，该方法包括：培养能够在培养基中产生氧化酶的微生物; 并从培养物中收集氧化酶。 此外，本发明提供了编码新型果糖基肽氧化酶的果糖基肽氧化酶基因，其中将基因插入载体DNA中的重组DNA，以及生产新型果糖基氧化酶的方法，所述方法包括：在培养基中培养， 包含该基因的转化体或转导体; 并从培养物中收集新颖的果糖基氧化酶。

公开(公告)号：US20050244926A1

公开(公告)日：2005-11-03

申请号：US11073626

申请日：2005-03-08

申请人： Keiko Kurosawa ,  Kozo Hirokawa ,  Naoki Kajiyama

发明人： Keiko Kurosawa ,  Kozo Hirokawa ,  Naoki Kajiyama

IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/04 ,  C12N9/06 ,  C12N15/53 ,  C12R1/645 ,  C07H21/04 ,  C12N1/16 ,  C12N15/74 ,  C12P21/06

CPC分类号： C12N9/0022

摘要： The present invention has an object of providing a novel fructosyl peptide oxidase having superior physicochemical properties such as stability that is useful as an enzyme for clinical diagnosis, and an object of providing a method for producing the fructosyl peptide oxidase. A novel fructosyl peptide oxidase having physicochemical properties useful as an enzyme for clinical diagnosis, and a method for producing a novel fructosyl peptide oxidase are provided herein, the method comprising: culturing a microorganism capable of producing the oxidase in a medium; and collecting the oxidase from the culture. Furthermore, a fructosyl peptide oxidase gene coding for a novel fructosyl peptide oxidase, recombinant DNA wherein the gene is inserted into vector DNA, and a method for producing a novel fructosyl peptide oxidase are provided herein, the method comprising: culturing, in a medium, a transformant or a transductant including the gene; and collecting the novel fructosyl peptide oxidase from the culture.

摘要翻译： 本发明的目的是提供具有优异的物理化学性质的新型果糖基氧化酶，例如可用作临床诊断用酶的稳定性，以及提供生产果糖基氧化酶的方法的目的。 本发明提供了具有用作临床诊断用酶的物理化学特性的新型果糖基氧化酶，以及生产新型果糖基氧化酶的方法，该方法包括：培养能够在培养基中产生氧化酶的微生物; 并从培养物中收集氧化酶。 此外，本发明提供了编码新型果糖基肽氧化酶的果糖基肽氧化酶基因，其中将基因插入载体DNA中的重组DNA，以及生产新型果糖基氧化酶的方法，所述方法包括：在培养基中培养， 包含该基因的转化体或转导体; 并从培养物中收集新颖的果糖基氧化酶。

公开(公告)号：US20100291623A1

公开(公告)日：2010-11-18

申请号：US12786005

申请日：2010-05-24

申请人： Kozo HIROKAWA ,  Keiko Kurosawa ,  Naoki Kajiyama

发明人： Kozo HIROKAWA ,  Keiko Kurosawa ,  Naoki Kajiyama

IPC分类号： C12P21/06

CPC分类号： C12P21/02 ,  C12P21/06

摘要： The present invention relates to a method for producing α-glycated dipeptide, which comprises causing protease to act on N-terminal-glycated peptide or N-terminal-glycated protein. The present invention further relates to a method for determining the amount of α-glycated dipeptide, which comprises causing a fructosyl peptide oxidase to act on the α-glycated dipeptide obtained by the above method and then determining the amount of the thus generated hydrogen peroxide. According to the present invention, a method for producing α-glycated dipeptide is provided, which enables the simple, rapid, and efficient production of α-glycated dipeptide from glycated protein or glycated peptide. Furthermore, according to the present invention, a method for determining the amount of α-glycated dipeptide is provided, which enables to determine the amount of α-glycated dipeptide in a highly precise manner within a short time period.

摘要翻译： 本发明涉及α-糖化二肽的制备方法，其包括使蛋白酶作用于N-末端糖基化肽或N-末端糖基化蛋白质。 本发明还涉及确定α-糖化二肽的量的方法，其包括使果糖基肽氧化酶作用于通过上述方法得到的α-糖化二肽，然后测定由此产生的过氧化氢的量。 根据本发明，提供了α-糖化二肽的制造方法，其能够从糖化蛋白质或糖化肽中简单，快速，有效地制备α-糖化二肽。 此外，根据本发明，提供了用于测定α-糖化二肽量的方法，其能够在短时间内以高度精确的方式测定α-糖化二肽的量。

公开(公告)号：US07419813B2

公开(公告)日：2008-09-02

申请号：US11073626

申请日：2005-03-08

申请人： Keiko Kurosawa ,  Kozo Hirokawa ,  Naoki Kajiyama

发明人： Keiko Kurosawa ,  Kozo Hirokawa ,  Naoki Kajiyama

IPC分类号： C12N9/02 ,  C12N1/20 ,  C12N15/00 ,  C12Q1/26 ,  C12Q1/00 ,  C12P7/00 ,  C07H21/04 ,  C12Q1/68 ,  C12P21/04

CPC分类号： C12N9/0022

摘要： The present invention has an object of providing a novel fructosyl peptide oxidase having superior physicochemical properties such as stability that is useful as an enzyme for clinical diagnosis, and an object of providing a method for producing the fructosyl peptide oxidase. A novel fructosyl peptide oxidase having physicochemical properties useful as an enzyme for clinical diagnosis, and a method for producing a novel fructosyl peptide oxidase are provided herein, the method comprising: culturing a microorganism capable of producing the oxidase in a medium; and collecting the oxidase from the culture. Furthermore, a fructosyl peptide oxidase gene coding for a novel fructosyl peptide oxidase, recombinant DNA wherein the gene is inserted into vector DNA, and a method for producing a novel fructosyl peptide oxidase are provided herein, the method comprising: culturing, in a medium, a transformant or a transductant including the gene; and collecting the novel fructosyl peptide oxidase from the culture.

摘要翻译： 本发明的目的是提供具有优异的物理化学性质的新型果糖基氧化酶，例如可用作临床诊断用酶的稳定性，以及提供生产果糖基氧化酶的方法的目的。 本发明提供了具有用作临床诊断用酶的物理化学特性的新型果糖基氧化酶，以及生产新型果糖基氧化酶的方法，该方法包括：培养能够在培养基中产生氧化酶的微生物; 并从培养物中收集氧化酶。 此外，本发明提供了编码新型果糖基肽氧化酶的果糖基肽氧化酶基因，其中将基因插入载体DNA中的重组DNA，以及生产新型果糖基氧化酶的方法，该方法包括：在培养基中培养， 包含该基因的转化体或转导体; 并从培养物中收集新颖的果糖基氧化酶。

公开(公告)号：US07101694B2

公开(公告)日：2006-09-05

申请号：US10333740

申请日：2001-07-26

申请人： Kozo Hirokawa ,  Keiko Kurosawa ,  Naoki Kajiyama

发明人： Kozo Hirokawa ,  Keiko Kurosawa ,  Naoki Kajiyama

IPC分类号： C12P9/02 ,  C12N15/00 ,  C12N1/20 ,  C12D21/06 ,  C07H21/04

CPC分类号： C12N9/0004 ,  C07K14/43563

摘要： The present invention relates to:an isolated or synthesized gene, which encodes a protein comprising the amino acid sequence represented by SEQ ID NO: 2,an isolated or synthesized gene, which encodes a protein comprising an amino acid sequence comprising a deletion, substitution or addition of one or more amino acids with respect to the amino acid sequence represented by SEQ ID NO: 2 and being capable of regenerating luciferin,an isolated or synthesized gene, which hybridizes with the complementary strand sequence of a DNA comprising the nucleotide sequence represented by SEQ ID NO: 1 under stringent conditions and encodes a protein capable of regenerating luciferin,a recombinant DNA, which is characterized in that the above-described isolated or synthesized gene is inserted into a vector DNA,a transformant or transductant comprising the above-described recombinant DNA, anda process for producing a protein capable of regenerating luciferin, which is characterized in that the method comprises culturing the above-described transformant or transductant in a medium and collecting therefrom the protein capable of regenerating luciferin.

摘要翻译： 本发明涉及：分离或合成的基因，其编码包含SEQ ID NO：2所示的氨基酸序列的蛋白质，分离或合成的基因，其编码包含缺失，取代或取代的氨基酸序列的蛋白质 相对于SEQ ID NO：2所示的氨基酸序列添加一个或多个氨基酸，并且能够再生荧光素，一种分离或合成的基因，其与包含以下的核苷酸序列的DNA的互补链序列杂交： SEQ ID NO：1，并编码能够再生荧光素的蛋白质，重组DNA，其特征在于将上述分离或合成的基因插入载体DNA，转化体或包含上述的转导体的转导体 重组DNA，以及能够再生荧光素的蛋白质的制造方法，其特征在于， od包括在培养基中培养上述转化体或转导体，并从中收集能够再生萤光素的蛋白质。

公开(公告)号：US06838270B1

公开(公告)日：2005-01-04

申请号：US10089986

申请日：2000-09-22

申请人： Keiko Kurosawa ,  Naoki Kajiyama

发明人： Keiko Kurosawa ,  Naoki Kajiyama

IPC分类号： C12N15/09 ,  C07K14/435 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/12 ,  C12N15/53 ,  C12P21/02 ,  C12R1/91 ,  C12N9/02

CPC分类号： C07K14/435 ,  C07K14/43563

摘要： A gene encoding a protein which is capable of regenerating luciferin by acting on oxyluciferin and D-cysteine and thus regenerating luciferin; the above gene originating in a luminous organism; a protein encoded by the above gene; and a process for producing a protein capable of regenerating luciferin characterized by comprising culturing a transformant or a transductant having the above gene transferred therein and collecting the protein capable of regenerating luciferin from the culture. Thus, the protein capable of regenerating luciferin can be efficiently produced, which brings about a great industrial advantage.

公开(公告)号：US20120295252A1

公开(公告)日：2012-11-22

申请号：US13134899

申请日：2011-06-20

申请人： Shigeya Suzuki ,  Yukako Kodama ,  Keiko Kurosawa ,  Eiry Kobatake ,  Masayasu Mie

发明人： Shigeya Suzuki ,  Yukako Kodama ,  Keiko Kurosawa ,  Eiry Kobatake ,  Masayasu Mie

IPC分类号： C12N9/02 ,  G01N21/76

CPC分类号： C12Q1/66

摘要： A composition for analyzing nucleic acid that contains luciferase for which reactivity to dATP is equal to or less than 1/400 reactivity to ATP. A method for analyzing nucleic acid that comprises the use of the composition. A kit for analyzing nucleic acid comprising the composition. The method provides for an inexpensive, highly accurate and highly sensitive nucleic acid analysis that uses dATP instead of an expensive reagent having a low reactivity to DNA polymerase.

摘要翻译： 用于分析含有对dATP的反应性等于或小于对ATP的反应性的反应性的荧光素酶的核酸的组合物。 一种分析包含使用该组合物的核酸的方法。 用于分析包含该组合物的核酸的试剂盒。 该方法提供使用dATP而不是对DNA聚合酶具有低反应性的昂贵试剂的廉价，高度准确和高度灵敏的核酸分析。