利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

19 条记录 (耗时 0.023 秒)

    METHOD FOR HIGHLY SENSITIVE NUCLEIC ACID DETECTION USING NANOPORE AND NON-SPECIFIC NUCLEIC ACID BINDING AGENT
    1.
    发明申请
    METHOD FOR HIGHLY SENSITIVE NUCLEIC ACID DETECTION USING NANOPORE AND NON-SPECIFIC NUCLEIC ACID BINDING AGENT 审中-公开
    使用纳米和非特异性核酸结合剂进行敏感性核酸检测的方法

    公开(公告)号：US20090169431A1

    公开(公告)日：2009-07-02

    申请号：US12364235

    申请日：2009-02-02

    申请人： Kui-hyun Kim ,  Jun-hong Min ,  In-ho Lee ,  Ah-gi Kim

    发明人： Kui-hyun Kim ,  Jun-hong Min ,  In-ho Lee ,  Ah-gi Kim

    IPC分类号： G01N27/00

    CPC分类号： B82Y5/00 ,  C12Q1/6825 ,  Y10S436/806 ,  Y10T436/143333 ,  C12Q2565/631

    摘要： Provided is a method of sensitively detecting nucleic acids in a nucleic acid sample, the method comprising: contacting the sample comprising the nucleic acid with a non-specific nucleic acid binding agent in an electrically conductive fluid medium; contacting the sample comprising the nucleic acid bound to the agent with a nanopore; and applying a voltage to the nanopore and monitoring a current change through the nanopore. The nucleic acid can be sensitively detected because a change in current amplitude through the nanopore is greater than when nucleic aid detection is performed without using an intercalator.

    摘要翻译： 提供了一种敏感地检测核酸样品中的核酸的方法，所述方法包括：使包含核酸的样品与导电流体培养基中的非特异性核酸结合剂接触; 使包含与试剂结合的核酸的样品与纳米孔接触; 并向纳米孔施加电压并监测通过纳米孔的电流变化。 可以灵敏地检测核酸，因为通过纳米孔的电流振幅的变化大于当不使用嵌入剂进行核酸检测时。

    Method for highly sensitive nucleic acid detection using nanopore and non-specific nucleic acid-binding agent
    2.
    发明授权
    Method for highly sensitive nucleic acid detection using nanopore and non-specific nucleic acid-binding agent 失效
    使用纳米孔和非特异性核酸结合剂的高灵敏度核酸检测方法

    公开(公告)号：US07504261B2

    公开(公告)日：2009-03-17

    申请号：US11414022

    申请日：2006-04-28

    申请人： Kui-hyun Kim ,  Jun-hong Min ,  In-ho Lee ,  Ah-gi Kim

    发明人： Kui-hyun Kim ,  Jun-hong Min ,  In-ho Lee ,  Ah-gi Kim

    IPC分类号： G01N33/00 ,  G01N33/53 ,  C07H21/04

    CPC分类号： B82Y5/00 ,  C12Q1/6825 ,  Y10S436/806 ,  Y10T436/143333 ,  C12Q2565/631

    摘要： Provided is a method of sensitively detecting nucleic acids in a nucleic acid sample, the method comprising: contacting the sample comprising the nucleic acid with a non-specific nucleic acid binding agent in an electrically conductive fluid medium; contacting the sample comprising the nucleic acid bound to the agent with a nanopore; and applying a voltage to the nanopore and monitoring a current change through the nanopore. The nucleic acid can be sensitively detected because a change in current amplitude through the nanopore is greater than when nucleic aid detection is performed without using an intercalator.

    摘要翻译： 提供了一种敏感地检测核酸样品中的核酸的方法，所述方法包括：使包含核酸的样品与导电流体培养基中的非特异性核酸结合剂接触; 使包含与试剂结合的核酸的样品与纳米孔接触; 并向纳米孔施加电压并监测通过纳米孔的电流变化。 可以灵敏地检测核酸，因为通过纳米孔的电流振幅的变化大于当不使用嵌入剂进行核酸检测时。

    Method of separating biomolecules using nanopore
    4.
    发明申请
    Method of separating biomolecules using nanopore 审中-公开
    使用纳米孔分离生物分子的方法

    公开(公告)号：US20060183112A1

    公开(公告)日：2006-08-17

    申请号：US11335246

    申请日：2006-01-19

    申请人： Jun-hong Min ,  Su-hyeon Kim ,  In-ho Lee ,  Kui-hyun Kim ,  Seung-yeon Yang

    发明人： Jun-hong Min ,  Su-hyeon Kim ,  In-ho Lee ,  Kui-hyun Kim ,  Seung-yeon Yang

    IPC分类号： C12Q1/70 ,  C12Q1/68

    CPC分类号： G01N33/5438 ,  G01N33/48721

    摘要： Provided is a method of separating particles, the method comprising: forming a first chamber and a second chamber separated by an interface with a pore, wherein the first and second chambers have electrodes with different polarities; placing particles to which a target biomolecule is bound from particles to which the target biomolecule is not bound in the first chamber; applying a voltage which has the same polarity as that of the target biomolecule to the electrode of the first chamber, and a voltage which has an opposite charge to that of the target biomolecule to the electrode of the second chamber; and translocating only the particles to which the target biomolecule is bound from the first chamber to the second chamber through the pore. Conventionally, the size of a pore is used to separate biomolecules. However, effective separation is difficult to achieve because the manufacture of a pore with a diameter of less than 10 nm, small enough to separate biomolecule, is not easy. Therefore, signal separation and data analysis must be required. However, in the present method, physical movement induced by the charge of biomolecules is used to effectively separate the biomolecules, thus obtaining a high signal to noise ratio. As a result, additional data analysis is not required.

    摘要翻译： 提供了一种分离颗粒的方法，该方法包括：形成由与孔的界面分隔开的第一室和第二室，其中第一和第二室具有不同极性的电极; 在第一室中放置靶生物分子所结合的颗粒与目标生物分子不结合的颗粒; 将具有与目标生物分子相同极性的电压施加到第一室的电极，以及将具有与靶生物分子相反的电荷的电压施加到第二室的电极; 并且仅将来自第一室的目标生物分子结合的颗粒通过孔转移到第二室。 通常，使用孔的大小来分离生物分子。 然而，由于直径小于10nm的孔的制造足够小以分离生物分子，所以难以实现有效的分离，这是不容易的。 因此，必须要求信号分离和数据分析。 然而，在本方法中，由生物分子的电荷引起的身体运动用于有效分离生物分子，从而获得高的信噪比。 因此，不需要额外的数据分析。

    Sensing switch and detecting method using the same
    7.
    发明授权
    Sensing switch and detecting method using the same 失效
    感应开关及使用其的检测方法

    公开(公告)号：US07510865B2

    公开(公告)日：2009-03-31

    申请号：US11347185

    申请日：2006-02-03

    申请人： Kyu-tae Yoo ,  Joon-ho Kim ,  Jun-hong Min ,  Sung-ouk Jung ,  Ji-na Namgoong ,  Kui-hyun Kim ,  Jeo-young Shim ,  Kak Namkoong

    发明人： Kyu-tae Yoo ,  Joon-ho Kim ,  Jun-hong Min ,  Sung-ouk Jung ,  Ji-na Namgoong ,  Kui-hyun Kim ,  Jeo-young Shim ,  Kak Namkoong

    IPC分类号： C12M1/34

    CPC分类号： G01N33/54373

    摘要： Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region on an upper surface of an end portion of the sensing plate; an electric or magnetic field generation device that induces deflection of the sensing plate when a receptor bound to the receptor binding region is selectively bound to an electrically or magnetically active ligand; and a pair of switching electrodes that are separated by a predetermined distance and is connected when the sensing plate contacts the substrate due to the deflection of the sensing plate. A target material need not be labelled, a signal processing of a fluorescent or electrical detection signal using an analysis apparatus is not required, and a signal can be directly decoded by confirming whether a current flows through the switch.

    摘要翻译： 提供了一种感测开关和使用该感测开关的感测方法。 感测开关包括：基板; 底物上的支撑物; 感测板，其与所述支撑体的一侧连接并且与所述基板平行预定距离; 在感测板的端部的上表面上的受体结合区域; 当与受体结合区结合的受体选择性地结合到电或磁性活性配体时，引起感测板偏转的电场或磁场产生装置; 以及一对分离预定距离的开关电极，并且当感测板由于感测板的偏转而与基板接触时被连接。 目标材料不需要标注，不需要使用分析装置的荧光或电检测信号的信号处理，并且可以通过确认电流是否流过开关直接解码信号。

    Sensing switch and detecting method using the same
    8.
    发明授权
    Sensing switch and detecting method using the same 有权
    感应开关及使用其的检测方法

    公开(公告)号：US07981666B2

    公开(公告)日：2011-07-19

    申请号：US12833095

    申请日：2010-07-09

    申请人： Kyu-tae Yoo ,  Joon-ho Kim ,  Jun-hong Min ,  Sung-ouk Jung ,  Ji-na Namgoong ,  Kui-hyun Kim ,  Jeo-young Shim ,  Kak Namkoong

    发明人： Kyu-tae Yoo ,  Joon-ho Kim ,  Jun-hong Min ,  Sung-ouk Jung ,  Ji-na Namgoong ,  Kui-hyun Kim ,  Jeo-young Shim ,  Kak Namkoong

    IPC分类号： C12M3/00

    CPC分类号： G01N33/54373

    摘要： Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region on an upper surface of an end portion of the sensing plate; an electric or magnetic field generation device that induces deflection of the sensing plate when a receptor bound to the receptor binding region is selectively bound to an electrically or magnetically active ligand; and a pair of switching electrodes that are separated by a predetermined distance and is connected when the sensing plate contacts the substrate due to the deflection of the sensing plate. A target material need not be labelled, a signal processing of a fluorescent or electrical detection signal using an analysis apparatus is not required, and a signal can be directly decoded by confirming whether a current flows through the switch.

    摘要翻译： 提供了一种感测开关和使用该感测开关的感测方法。 感测开关包括：基板; 底物上的支撑物; 感测板，其与所述支撑体的一侧连接并且与所述基板平行预定距离; 在感测板的端部的上表面上的受体结合区域; 当与受体结合区结合的受体选择性地结合到电或磁性活性配体时，引起感测板偏转的电场或磁场产生装置; 以及一对分离预定距离的开关电极，并且当感测板由于感测板的偏转而与基板接触时被连接。 目标材料不需要标注，不需要使用分析装置的荧光或电检测信号的信号处理，并且可以通过确认电流是否流过开关直接解码信号。

    Sensing switch and detecting method using the same
    9.
    发明申请
    Sensing switch and detecting method using the same 失效

    公开(公告)号：US20060177874A1

    公开(公告)日：2006-08-10

    申请号：US11347185

    申请日：2006-02-03

    申请人： Kyu-tae Yoo ,  Joon-ho Kim ,  Jun-hong Min ,  Sung-ouk Jung ,  Ji-na Namgoong ,  Kui-hyun Kim ,  Jeo-young Shim

    发明人： Kyu-tae Yoo ,  Joon-ho Kim ,  Jun-hong Min ,  Sung-ouk Jung ,  Ji-na Namgoong ,  Kui-hyun Kim ,  Jeo-young Shim

    IPC分类号： G01N33/53 ,  C12M1/34

    CPC分类号： G01N33/54373

    摘要： Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region on an upper surface of an end portion of the sensing plate; an electric or magnetic field generation device that induces deflection of the sensing plate when a receptor bound to the receptor binding region is selectively bound to an electrically or magnetically active ligand; and a pair of switching electrodes that are separated by a predetermined distance and is connected when the sensing plate contacts the substrate due to the deflection of the sensing plate. A target material need not be labelled, a signal processing of a fluorescent or electrical detection signal using an analysis apparatus is not required, and a signal can be directly decoded by confirming whether a current flows through the switch.

    Sensing switch and detecting method using the same
    10.
    发明授权
    Sensing switch and detecting method using the same 有权
    感应开关及使用其的检测方法

    公开(公告)号：US07790440B2

    公开(公告)日：2010-09-07

    申请号：US12272183

    申请日：2008-11-17

    申请人： Kyu-tae Yoo ,  Joon-ho Kim ,  Jun-hong Min ,  Sung-ouk Jung ,  Ji-na Namgoong ,  Kui-hyun Kim ,  Jeo-young Shim ,  Kak Namkoong

    发明人： Kyu-tae Yoo ,  Joon-ho Kim ,  Jun-hong Min ,  Sung-ouk Jung ,  Ji-na Namgoong ,  Kui-hyun Kim ,  Jeo-young Shim ,  Kak Namkoong

    IPC分类号： C12M3/00

    CPC分类号： G01N33/54373

    摘要： Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region on an upper surface of an end portion of the sensing plate; an electric or magnetic field generation device that induces deflection of the sensing plate when a receptor bound to the receptor binding region is selectively bound to an electrically or magnetically active ligand; and a pair of switching electrodes that are separated by a predetermined distance and is connected when the sensing plate contacts the substrate due to the deflection of the sensing plate. A target material need not be labelled, a signal processing of a fluorescent or electrical detection signal using an analysis apparatus is not required, and a signal can be directly decoded by confirming whether a current flows through the switch.

    摘要翻译： 提供了一种感测开关和使用该感测开关的感测方法。 感测开关包括：基板; 底物上的支撑物; 感测板，其与所述支撑体的一侧连接并且与所述基板平行预定距离; 在感测板的端部的上表面上的受体结合区域; 当与受体结合区结合的受体选择性地结合到电或磁性活性配体时，引起感测板偏转的电场或磁场产生装置; 以及一对分离预定距离的开关电极，并且当感测板由于感测板的偏转而与基板接触时被连接。 目标材料不需要标注，不需要使用分析装置的荧光或电检测信号的信号处理，并且可以通过确认电流是否流过开关直接解码信号。