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    Nucleic acid purification method using hydrogen bonding and electric field
    2.
    发明申请
    Nucleic acid purification method using hydrogen bonding and electric field 审中-公开
    使用氢键和电场的核酸纯化方法

    公开(公告)号：US20060118417A1

    公开(公告)日：2006-06-08

    申请号：US11281226

    申请日：2005-11-16

    申请人： Young-a Kim ,  Jun-hong Min ,  Kui-hyun Kim ,  Myo-yong Lee ,  Su-hyeon Kim ,  Young-nam Kwon ,  Jeong-gun Lee ,  Joon-ho Kim

    发明人： Young-a Kim ,  Jun-hong Min ,  Kui-hyun Kim ,  Myo-yong Lee ,  Su-hyeon Kim ,  Young-nam Kwon ,  Jeong-gun Lee ,  Joon-ho Kim

    IPC分类号： C07H21/04

    CPC分类号： C07H21/04

    摘要： Provided is a method of purifying nucleic acids using hydrogen bonding and an electric field, including: bringing a sample containing target nucleic acids into contact with an electrode coated with a material capable of forming hydrogen bonds with the target nucleic acids; applying a positive voltage to the electrode to move the target nucleic acids closer to the electrode so as to form hydrogen bonds with the material on the electrode; washing the electrode; and applying to the electrode a negative voltage to elute the bound target nucleic acids. According to the method, selectivity to nucleic acids and proteins increases due to hydrogen bonding, nucleic acid purification is possible within a short time through an electric field, and the bound nucleic acids can be efficiently eluted.

    摘要翻译： 提供了使用氢键和电场来净化核酸的方法，包括：使含有靶核酸的样品与涂覆有能够与靶核酸形成氢键的材料接触; 向电极施加正电压以使靶核酸更靠近电极以与电极上的材料形成氢键; 清洗电极; 并向电极施加负电压以洗脱结合的靶核酸。 根据该方法，由于氢键，核酸和蛋白质的选择性增加，核酸纯化可以在短时间内通过电场进行，并且结合的核酸可以被有效地洗脱。

    Biochemical analyzer and method of controlling internal temperature of the biochemical analyzer
    6.
    发明授权
    Biochemical analyzer and method of controlling internal temperature of the biochemical analyzer 有权
    生化分析仪和生化分析仪内部温度控制方法

    公开(公告)号：US07943088B2

    公开(公告)日：2011-05-17

    申请号：US11972708

    申请日：2008-01-11

    申请人： Jeong-gun Lee ,  Yong-moo Shin ,  Kui-hyun Kim ,  Jin-han Chung

    发明人： Jeong-gun Lee ,  Yong-moo Shin ,  Kui-hyun Kim ,  Jin-han Chung

    IPC分类号： C12Q1/68 ,  G01N15/06

    CPC分类号： B01L7/52 ,  B01L3/5027 ,  B01L9/527 ,  B01L2300/0681 ,  B01L2300/0806 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1844 ,  B01L2300/1883 ,  B01L2400/0409 ,  G01N35/00069 ,  G01N2035/00346 ,  Y10T436/11 ,  Y10T436/25

    摘要： Provided is a biochemical analyzer in which a microfluidic biochemical assay may be performed. The analyzer includes: a microfluidic device loading space including a microfluidic device supporting unit detachably supporting a microfluidic device including an energy application region in which an energy is applied; an energy source loading space including an energy source applying the energy to the radiation application region; and an isolation wall isolating the microfluidic device loading space and the energy source loading space to prevent heat transfer between the microfluidic device loading space and the energy source loading space and including a window through which the energy can be transmitted. A method of controlling an internal temperature of the biochemical analyzer is also provided.

    摘要翻译： 提供了可以进行微流体生化测定的生化分析仪。 该分析仪包括：微流体装置装载空间，其包括微流体装置支撑单元，其可拆卸地支撑包括其中施加能量的能量施加区域的微流体装置; 能量源装载空间，其包括将能量施加到所述辐射施加区域的能量源; 隔离壁隔离微流体装置加载空间和能量源装载空间，以防止微流体装置加载空间与能量源装载空间之间的热传递并且包括能够传输能量的窗口。 还提供了一种控制生化分析仪内部温度的方法。

    Method and apparatus for the rapid disruption of cells or viruses using micro magnetic beads and laser
    8.
    发明授权
    Method and apparatus for the rapid disruption of cells or viruses using micro magnetic beads and laser 有权
    使用微磁珠和激光快速破坏细胞或病毒的方法和装置

    公开(公告)号：US07855069B2

    公开(公告)日：2010-12-21

    申请号：US11253541

    申请日：2005-10-19

    申请人： Jeong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Myo-yong Lee ,  Shin-i Yoo ,  Yeon-ja Cho ,  Kwang-ho Cheong ,  Chang-eun Yoo ,  Seung-yeon Yang

    发明人： Jeong-gun Lee ,  Young-nam Kwon ,  Young-a Kim ,  Myo-yong Lee ,  Shin-i Yoo ,  Yeon-ja Cho ,  Kwang-ho Cheong ,  Chang-eun Yoo ,  Seung-yeon Yang

    IPC分类号： C12M1/42 ,  C12M1/33

    CPC分类号： C12M47/06 ,  B01F11/0266 ,  B01F13/0059 ,  B01L3/502761 ,  B01L2300/0816 ,  C12N1/066 ,  C12N13/00

    摘要： A method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser are provided. According to the method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC.

    摘要翻译： 提供了使用微磁珠和激光快速破坏细胞或病毒的方法和装置。 根据使用微磁珠和激光快速破坏细胞或病毒的方法和装置，可以在40秒内进行细胞裂解，使用激光二极管可以使装置小型化，DNA纯化步骤可以直接在 细胞或病毒的破坏以及含有DNA的溶液可以转移到随后的步骤中，随后的反应的抑制剂与细胞碎片和珠子一起用电磁铁去除。 另外，通过细胞裂解芯片，解决了蒸发问题，可以通过磁珠将振动有效地转移到细胞中，通过疏水处理芯片的内表面和细胞来解决粗糙表面上的微流体问题 裂解芯片可应用于LOC。

    Method for purification of nucleic acids by phase separation using laser and beads
    9.
    发明授权
    Method for purification of nucleic acids by phase separation using laser and beads 有权
    使用激光和珠子通过相分离纯化核酸的方法

    公开(公告)号：US07429470B2

    公开(公告)日：2008-09-30

    申请号：US11265461

    申请日：2005-11-02

    申请人： Jeong-gun Lee ,  Young-nam Kwon ,  Young-a Kim

    发明人： Jeong-gun Lee ,  Young-nam Kwon ,  Young-a Kim

    IPC分类号： C12N13/00 ,  C12N1/08 ,  G01N33/00 ,  C07H21/02

    CPC分类号： B01L3/502761 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0838 ,  B01L2300/087 ,  B01L2400/0633 ,  G01N35/0098 ,  Y10T436/143333

    摘要： An apparatus and method for purification of nucleic acids of cells or viruses are provided. The nucleic acid purification apparatus includes: a cell lysis capillary having a sample inlet through which samples and magnetic beads are introduced; a vibrator attached to the capillary and mixing the samples and the magnetic beads in the capillary; a laser generator attached to the capillary and supplying a laser to the capillary; and a magnetic force generator attached to the capillary and fixing the magnetic beads to a capillary wall. According to the method and apparatus, PCR yield can be increased since PCR inhibitors can be readily removed by means of a phase separation in a capillary. The use of an electromagnet ensures the removal of the PCR inhibitors. In addition, since cell lysis and DNA purification process can be simultaneously performed, LOC steps can be reduced.

    摘要翻译： 提供了用于纯化细胞或病毒核酸的装置和方法。 核酸纯化装置包括：细胞裂解毛细管，其具有样品和磁珠引入的样品入口; 连接到毛细管并将样品和磁珠混合在毛细管中的振动器; 激光发生器，附接到毛细管并向毛细管提供激光; 以及附接到毛细管并将磁珠固定到毛细管壁的磁力发生器。 根据该方法和装置，可以增加PCR产量，因为通过毛细管中的相分离可以容易地除去PCR抑制剂。 使用电磁铁确保去除PCR抑制剂。 此外，由于可以同时进行细胞裂解和DNA纯化过程，因此可以减少LOC步骤。

    Apparatus and method for purification of nucleic acids by phase separation using laser and beads
    10.
    发明申请
    Apparatus and method for purification of nucleic acids by phase separation using laser and beads 有权
    使用激光和珠子通过相分离纯化核酸的装置和方法

    公开(公告)号：US20060094051A1

    公开(公告)日：2006-05-04

    申请号：US11265461

    申请日：2005-11-02

    申请人： Jeong-gun Lee ,  Young-nam Kwon ,  Young-a Kim

    发明人： Jeong-gun Lee ,  Young-nam Kwon ,  Young-a Kim

    IPC分类号： C12Q1/68 ,  C12N1/08 ,  C12M1/34

    CPC分类号： B01L3/502761 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0668 ,  B01L2300/0838 ,  B01L2300/087 ,  B01L2400/0633 ,  G01N35/0098 ,  Y10T436/143333

    摘要： An apparatus and method for purification of nucleic acids of cells or viruses are provided. The nucleic acid purification apparatus includes: a cell lysis capillary having a sample inlet through which samples and magnetic beads are introduced; a vibrator attached to the capillary and mixing the samples and the magnetic beads in the capillary; a laser generator attached to the capillary and supplying a laser to the capillary; and a magnetic force generator attached to the capillary and fixing the magnetic beads to a capillary wall. According to the method and apparatus, PCR yield can be increased since PCR inhibitors can be readily removed by means of a phase separation in a capillary. The use of an electromagnet ensures the removal of the PCR inhibitors. In addition, since cell lysis and DNA purification process can be simultaneously performed, LOC steps can be reduced.

    摘要翻译： 提供了用于纯化细胞或病毒核酸的装置和方法。 核酸纯化装置包括：细胞裂解毛细管，其具有样品和磁珠引入的样品入口; 连接到毛细管并将样品和磁珠混合在毛细管中的振动器; 激光发生器附接到毛细管并向毛细管提供激光; 以及附接到毛细管并将磁珠固定到毛细管壁的磁力发生器。 根据该方法和装置，可以增加PCR产量，因为通过毛细管中的相分离可以容易地除去PCR抑制剂。 使用电磁铁确保去除PCR抑制剂。 此外，由于可以同时进行细胞裂解和DNA纯化过程，因此可以减少LOC步骤。