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公开(公告)号：US20130071898A1
公开(公告)日：2013-03-21
申请号：US13428585
申请日：2012-03-23
申请人： LARRY CAMERON ANTHONY , Hongxian He , Lixuan Lisa Huang , Daniel P. Okeefe , Arthur Leo Kruckeberg , Yougen Li , Lori Ann Maggio-Hall , Jessica Mcelvain , Mark J. Nelson , Ranjan Patnaik , Steven Cary Rothman
发明人： LARRY CAMERON ANTHONY , Hongxian He , Lixuan Lisa Huang , Daniel P. Okeefe , Arthur Leo Kruckeberg , Yougen Li , Lori Ann Maggio-Hall , Jessica Mcelvain , Mark J. Nelson , Ranjan Patnaik , Steven Cary Rothman
CPC分类号： C12P7/16 , C12N1/38 , C12N9/0006 , C12N15/81 , C12N15/815 , C12P7/42 , Y02E50/10 , Y02P20/52
摘要： Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.
摘要翻译： 本文提供重组酵母宿主细胞及其用于生产异丁醇的方法。 提供的酵母宿主细胞包含异丁醇生物合成途径和降低或消除的醛脱氢酶活性，降低或消除的乙酰乳酸还原酶活性中的至少一种; 或编码具有酮酸酸还原异构酶活性的多肽的异源多核苷酸。
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公开(公告)号：US20100081179A1
公开(公告)日：2010-04-01
申请号：US12569069
申请日：2009-09-29
摘要： Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe—S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe—S protein. Activities of heterologous fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe—S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.
摘要翻译： 工程化的酵母菌株具有增加Fe-S簇结合活性的异源蛋白质的活性。 酵母菌株内源性Fe-S蛋白的活性降低。 增加异源真菌或植物2Fe-2S二羟基酸脱水酶和Fe-S丙二醇脱水酶再活化酶的活性，以增加使用包括这些酶的生物合成途径（如缬氨酸，异亮氨酸，亮氨酸，泛酸（维生素B5））， 异丁醇，2-丁酮和2-丁醇。
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公开(公告)号：US20110124060A1
公开(公告)日：2011-05-26
申请号：US12893089
申请日：2010-09-29
CPC分类号： C12N9/88 , C12N9/0006 , C12N9/0008 , C12N9/1022 , C12N9/1205 , C12N15/81 , C12N15/815 , C12P7/16 , Y02E50/10
摘要： Crabtree positive yeast cells that have endogenous expressed pyruvate decarboxylase genes inactivated and an engineered biosynthetic pathway utilizing pyruvate were found to have improved growth and product yield when glucose repression was reduced. These cells were able to grow in media containing a high glucose concentration.
摘要翻译： 发现内源性表达的丙酮酸脱羧酶基因灭活的Crabtree阳性酵母细胞和利用丙酮酸的工程化生物合成途径在葡萄糖抑制降低时具有改善的生长和产物产率。 这些细胞能够在含有高葡萄糖浓度的培养基中生长。
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