-
公开(公告)号：US09790521B2
公开(公告)日：2017-10-17
申请号：US13428585
申请日：2012-03-23
申请人： Larry Cameron Anthony , Hongxian He , Lixuan Lisa Huang , Daniel P. Okeefe , Arthur Leo Kruckeberg , Yougen Li , Lori Ann Maggio-Hall , Jessica McElvain , Mark J. Nelson , Ranjan Patnaik , Steven Cary Rothman
发明人： Larry Cameron Anthony , Hongxian He , Lixuan Lisa Huang , Daniel P. Okeefe , Arthur Leo Kruckeberg , Yougen Li , Lori Ann Maggio-Hall , Jessica McElvain , Mark J. Nelson , Ranjan Patnaik , Steven Cary Rothman
CPC分类号： C12P7/16 , C12N1/38 , C12N9/0006 , C12N15/81 , C12N15/815 , C12P7/42 , Y02E50/10 , Y02P20/52
摘要： Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.
-
公开(公告)号：US20100158823A1
公开(公告)日：2010-06-24
申请号：US12632831
申请日：2009-12-08
CPC分类号： A61K47/48338 , A61K8/19 , A61K8/64 , A61K2800/94 , A61Q5/00 , A61Q5/065 , C07K14/001 , C07K2319/70 , G01N33/6803 , G06F19/16
摘要： Short single chain peptides having affinity for a target surface often lack the binding durability required for certain commercial applications. One way to improve durability is to promote multivalent binding by linking together binding sequences using peptide linkers. However, the resulting single chain binding peptides often suffer from linker entropy. It has been discovered that the use of rigid peptide linkers when linking together multiple binding sequences enhances the binding affinity of the resulting single chain peptide.
摘要翻译： 对目标表面具有亲和性的短单链肽通常缺乏某些商业应用所需的结合耐久性。 提高耐久性的一种方法是通过使用肽接头连接在一起的结合序列来促进多价结合。 然而，所得到的单链结合肽通常具有接头熵。 已经发现，当将多个结合序列连接在一起时，使用刚性肽接头增强所得单链肽的结合亲和力。
-
3.发明授权Mutant HPGG motif and HDASH motif delta-5 desaturases and their use in making polyunsaturated fatty acids 有权突变型HPGG基序和HDASH基序delta-5去饱和酶及其在制备多不饱和脂肪酸中的应用公开(公告)号：US08658413B2
公开(公告)日：2014-02-25
申请号：US13218673
申请日：2011-08-26
申请人： Melissa D. Bosak , Michael W. Bostick , Hongxian He , Seung-Pyo Hong , Dana M. Walters Pollak , Pamela L. Sharpe , Yehong Jamie Wang , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
发明人： Melissa D. Bosak , Michael W. Bostick , Hongxian He , Seung-Pyo Hong , Dana M. Walters Pollak , Pamela L. Sharpe , Yehong Jamie Wang , Zhixiong Xue , Narendra S. Yadav , Hongxiang Zhang , Quinn Qun Zhu
CPC分类号： C12N9/001 , C12P7/6472 , C12Y103/01035
摘要： Mutant delta-5 desaturases, having the ability to convert dihomo-gamma-linolenic acid [DGLA; 20:3 omega-6] to arachidonic acid [ARA; 20:4 omega-6] and/or eicosatetraenoic acid [ETA; 20:4 omega-3] to eicosapentaenoic acid [EPA; 20:5 omega-3] and possessing at least one mutation within the HPGG (SEQ ID NO:7) motif of the cytochome b5-like domain and at least one mutation within the HDASH (SEQ ID NO:8) motif are disclosed. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-5 desaturases, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”], are also disclosed.
摘要翻译： 具有将dihomo-γ-亚麻酸转化的能力的变体delta-5去饱和酶[DGLA; 20：3ω-6]花生四烯酸[ARA; 20：4ω-6]和/或二十碳四烯酸[ETA; 20：4ω-3]至二十碳五烯酸[EPA; 20：5ω-3]，并且在细胞色素b5样结构域的HPGG（SEQ ID NO：7）基序和HDASH（SEQ ID NO：8）基序内的至少一个突变中具有至少一个突变。 还公开了分离的核酸片段和包含编码Δ-5去饱和酶的片段的重组构建物，以及制备长链多不饱和脂肪酸[“PUFA”]的方法。
-
4.发明申请KETO-ISOVALERATE DECARBOXYLASE ENZYMES AND METHODS OF USE THEREOF 有权KETO-ISOVALERATE DECARBOXYLASE ENZYMES及其使用方法公开(公告)号：US20130203138A1
公开(公告)日：2013-08-08
申请号：US13560147
申请日：2012-07-27
申请人： Jessica McElvain , Daniel P. Okeefe , Brian James Paul , Mark S. Payne , Steven Cary Rothman , Hongxian He
发明人： Jessica McElvain , Daniel P. Okeefe , Brian James Paul , Mark S. Payne , Steven Cary Rothman , Hongxian He
CPC分类号： C12P7/16 , C12N9/0006 , C12P7/24 , Y02E50/10
摘要： Provided herein are polypeptides and polynucleotides encoding such polypeptides which have ketoisovalerate decarboxylase activity. Also provided are recombinant host cells comprising such polypeptides and polynucleotides and methods of use thereof.
摘要翻译： 本文提供了编码具有酮异戊酸脱羧酶活性的多肽的多肽和多核苷酸。 还提供了包含这种多肽和多核苷酸的重组宿主细胞及其使用方法。
-
5.发明授权Mutant delta-9 elongases and their use in making polyunsaturated fatty acids 有权突变型Δ-9延伸酶及其在制备多不饱和脂肪酸中的应用公开(公告)号：US08980589B2
公开(公告)日：2015-03-17
申请号：US13218591
申请日：2011-08-26
申请人： Michael W. Bostick , Hongxian He , Yougen Li , Quinn Qun Zhu
发明人： Michael W. Bostick , Hongxian He , Yougen Li , Quinn Qun Zhu
CPC分类号： C12N9/1029 , C12N15/815 , C12P7/6472 , C12Y203/01016
摘要： Mutant delta-9 elongases having the ability to convert linoleic acid [18:2, LA] to eicosadienoic acid [20:2, EDA] and/or α-linolenic [18:3, ALA] to eicosatrienoic acid [20:3, ETrA] are disclosed herein. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding mutant delta-9 elongases, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”] using these mutant delta-9 elongases in oleaginous yeast are also disclosed.
摘要翻译： 具有将亚油酸[18：2，LA]转化成二十碳烯酸[20：2，EDA]和/或α-亚麻酸[18：3，ALA]至二十碳三烯酸[20：3]的能力的突变型Δ-9延伸酶 ETrA]。 还公开了分离的核酸片段和包含编码突变型Δ-9延伸酶的这种片段的重组构建物，以及在含油酵母中使用这些突变型Δ-9延长酶制备长链多不饱和脂肪酸的方法[“PUFA”。
-
6.发明申请MUTANT DELTA-9 ELONGASES AND THEIR USE IN MAKING POLYUNSATURATED FATTY ACIDS 有权MUTANT DELTA-9 ELONGASES及其在制备聚氨酯脂肪酸中的应用公开(公告)号：US20120226062A1
公开(公告)日：2012-09-06
申请号：US13218591
申请日：2011-08-26
申请人： Michael W. Bostick , Hongxian He , Yougen Li , Quinn Qun Zhu
发明人： Michael W. Bostick , Hongxian He , Yougen Li , Quinn Qun Zhu
IPC分类号： C07C57/00 , C12N9/02 , C12N15/63 , C12N5/10 , C12P7/64 , C12N1/19 , C12N1/13 , C12N1/15 , C12N1/00 , C07H21/04 , C12N1/21
CPC分类号： C12N9/1029 , C12N15/815 , C12P7/6472 , C12Y203/01016
摘要： Mutant delta-9 elongases having the ability to convert linoleic acid [18:2, LA] to eicosadienoic acid [20:2, EDA] and/or α-linolenic [18:3, ALA] to eicosatrienoic acid [20:3, ETrA] are disclosed herein. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding mutant delta-9 elongases, along with a method of making long chain polyunsaturated fatty acids [“PUFAs”] using these mutant delta-9 elongases in oleaginous yeast are also disclosed.
摘要翻译： 具有将亚油酸[18：2，LA]转化成二十碳烯酸[20：2，EDA]和/或α-亚麻酸[18：3，ALA]至二十碳三烯酸[20：3]的能力的突变型Δ-9延伸酶 ETrA]。 还公开了分离的核酸片段和包含编码突变型Δ-9延伸酶的这种片段的重组构建物，以及在含油酵母中使用这些突变型Δ-9延长酶制备长链多不饱和脂肪酸的方法[“PUFA”。
-
公开(公告)号：US09238828B2
公开(公告)日：2016-01-19
申请号：US13560147
申请日：2012-07-27
申请人： Jessica McElvain , Daniel P O'Keefe , Brian James Paul , Mark S Payne , Steven Cary Rothman , Hongxian He
发明人： Jessica McElvain , Daniel P O'Keefe , Brian James Paul , Mark S Payne , Steven Cary Rothman , Hongxian He
CPC分类号： C12P7/16 , C12N9/0006 , C12P7/24 , Y02E50/10
摘要： Provided herein are polypeptides and polynucleotides encoding such polypeptides which have ketoisovalerate decarboxylase activity. Also provided are recombinant host cells comprising such polypeptides and polynucleotides and methods of use thereof.
摘要翻译： 本文提供了编码具有酮异戊酸脱羧酶活性的多肽的多肽和多核苷酸。 还提供了包含这种多肽和多核苷酸的重组宿主细胞及其使用方法。
-
公开(公告)号：US08697654B2
公开(公告)日：2014-04-15
申请号：US12632831
申请日：2009-12-08
CPC分类号： A61K47/48338 , A61K8/19 , A61K8/64 , A61K2800/94 , A61Q5/00 , A61Q5/065 , C07K14/001 , C07K2319/70 , G01N33/6803 , G06F19/16
摘要： Short single chain peptides having affinity for a target surface often lack the binding durability required for certain commercial applications. One way to improve durability is to promote multivalent binding by linking together binding sequences using peptide linkers. However, the resulting single chain binding peptides often suffer from linker entropy. It has been discovered that the use of rigid peptide linkers when linking together multiple binding sequences enhances the binding affinity of the resulting single chain peptide.
摘要翻译： 对目标表面具有亲和性的短单链肽通常缺乏某些商业应用所需的结合耐久性。 提高耐久性的一种方法是通过使用肽接头连接在一起的结合序列来促进多价结合。 然而，所得到的单链结合肽通常具有接头熵。 已经发现，当将多个结合序列连接在一起时，使用刚性肽接头增强所得单链肽的结合亲和力。
-
9.发明申请Mutant Delta-8 Desaturase Genes Engineered By Targeted Mutagensis and Their Use In Making Polyunsaturated Fatty Acids 审中-公开突变型Delta-8去饱和酶基因由目标突变体及其用于制备多不饱和脂肪酸公开(公告)号：US20110268864A1
公开(公告)日：2011-11-03
申请号：US13178292
申请日：2011-07-07
申请人： HOWARD GLENN DAMUDE , HONGXIAN HE , DER-ING LIAO , QUINN QUN ZHU
发明人： HOWARD GLENN DAMUDE , HONGXIAN HE , DER-ING LIAO , QUINN QUN ZHU
CPC分类号： C12N15/8247 , C12N9/0083
摘要： The present invention relates to mutant delta-8 desaturase genes, which have the ability to convert eicosadienoic acid [20:2 omega-6, EDA] to dihomo-γ-linolenic acid [20:3, DGLA] and/or eicosatrienoic acid [20:3 omega-3, ETrA] to eicosatetraenoic acid [20:3 omega-3, ETA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-8 desaturase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these mutant delta-8 desaturases in plants and oleaginous yeast are disclosed.
摘要翻译： 本发明涉及具有将二十碳二烯酸[20：2ω-6，EDA]转化为二高-γ-亚麻酸[20:3，DGLA]和/或二十碳三烯酸[...]的能力的突变体δ-8去饱和酶基因[ 20：3ω-3，ETrA]至二十碳四烯酸[20：3ω-3，ETA]。 公开了分离的核酸片段和重组构建体，其包含编码δ-8去饱和酶的片段以及使用植物中的这些突变型δ-8去饱和酶和油性酵母制备长链多不饱和脂肪酸（PUFA）的方法。
-
10.发明授权Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids 有权突变和Dgr; 8去饱和酶基因通过靶向诱变工程改造，并用于制备多不饱和脂肪酸公开(公告)号：US08026089B2
公开(公告)日：2011-09-27
申请号：US12726390
申请日：2010-03-18
申请人： Howard Glenn Damude , Hongxian He , Der-Ing Liao , Quinn Qun Zhu
发明人： Howard Glenn Damude , Hongxian He , Der-Ing Liao , Quinn Qun Zhu
IPC分类号： C07K14/00 , C12N9/02 , C12N5/10 , C12N1/15 , C12N5/04 , C12N1/21 , C12P7/64 , C12Q1/26 , C12P21/00
CPC分类号： C12N9/0083
摘要： The present invention relates to mutant Δ8 desaturase genes, which have the ability to convert eicosadienoic acid [20:2 ω-6, EDA] to dihomo-γ-linolenic acid [20:3, DGLA] and/or eicosatrienoic acid [20:3 ω-3, ETrA] to eicosatetraenoic acid [20:3 ω-3, ETA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ8 desaturase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these mutant Δ8 desaturases in plants and oleaginous yeast are disclosed.
摘要翻译： 本发明涉及具有将二十碳二烯酸[20:2ω-6，EDA]转化为二高-γ-亚麻酸[20:3，DGLA]和/或二十碳三烯酸[ 20：3ω-3，ETrA]至二十碳四烯酸[20：3ω-3，ETA]。 公开了分离的核酸片段和重组构建体，其包含编码“Dgr”8去饱和酶的片段以及使用这些突变体和Dgr。8去饱和酶在植物和油质酵母中制备长链多不饱和脂肪酸（PUFA）的方法。
-
-
-
-
-
-
-
-
-
搜索结果
17 条记录 (耗时 0.029 秒)
