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公开(公告)号：US09790521B2
公开(公告)日：2017-10-17
申请号：US13428585
申请日：2012-03-23
申请人： Larry Cameron Anthony , Hongxian He , Lixuan Lisa Huang , Daniel P. Okeefe , Arthur Leo Kruckeberg , Yougen Li , Lori Ann Maggio-Hall , Jessica McElvain , Mark J. Nelson , Ranjan Patnaik , Steven Cary Rothman
发明人： Larry Cameron Anthony , Hongxian He , Lixuan Lisa Huang , Daniel P. Okeefe , Arthur Leo Kruckeberg , Yougen Li , Lori Ann Maggio-Hall , Jessica McElvain , Mark J. Nelson , Ranjan Patnaik , Steven Cary Rothman
CPC分类号： C12P7/16 , C12N1/38 , C12N9/0006 , C12N15/81 , C12N15/815 , C12P7/42 , Y02E50/10 , Y02P20/52
摘要： Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.
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公开(公告)号：US20130071898A1
公开(公告)日：2013-03-21
申请号：US13428585
申请日：2012-03-23
申请人： LARRY CAMERON ANTHONY , Hongxian He , Lixuan Lisa Huang , Daniel P. Okeefe , Arthur Leo Kruckeberg , Yougen Li , Lori Ann Maggio-Hall , Jessica Mcelvain , Mark J. Nelson , Ranjan Patnaik , Steven Cary Rothman
发明人： LARRY CAMERON ANTHONY , Hongxian He , Lixuan Lisa Huang , Daniel P. Okeefe , Arthur Leo Kruckeberg , Yougen Li , Lori Ann Maggio-Hall , Jessica Mcelvain , Mark J. Nelson , Ranjan Patnaik , Steven Cary Rothman
CPC分类号： C12P7/16 , C12N1/38 , C12N9/0006 , C12N15/81 , C12N15/815 , C12P7/42 , Y02E50/10 , Y02P20/52
摘要： Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.
摘要翻译： 本文提供重组酵母宿主细胞及其用于生产异丁醇的方法。 提供的酵母宿主细胞包含异丁醇生物合成途径和降低或消除的醛脱氢酶活性，降低或消除的乙酰乳酸还原酶活性中的至少一种; 或编码具有酮酸酸还原异构酶活性的多肽的异源多核苷酸。
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3.发明申请REDUCTION OF 2,3-DIHYDROXY-2-METHYL BUTYRATE (DHMB) IN BUTANOL PRODUCTION 审中-公开在丁醇生产中减少2,3-二羟基-2-甲基丁酸（DHMB）公开(公告)号：US20120258873A1
公开(公告)日：2012-10-11
申请号：US13153866
申请日：2011-06-06
申请人： KATHARINE J. GIBSON , Arthur Leo Kruckeberg , Lori Ann Maggio-Hall , Mark J. Nelson , Ranjan Patnaik
发明人： KATHARINE J. GIBSON , Arthur Leo Kruckeberg , Lori Ann Maggio-Hall , Mark J. Nelson , Ranjan Patnaik
CPC分类号： C12Q1/025 , C12N9/0006 , C12P7/16 , C12Q1/26 , Y02E50/10
摘要： The invention relates generally to the field of industrial microbiology and butanol production. More specifically, the invention relates methods of reducing 2,3-dihydroxy-2-methyl butyrate (DHMB) in butanol production. DHMB can be reduced by inhibiting the reduction of acetolactate to DHMB, for example, by knocking out enzymes that catalyze the reduction or by removing DHMB during or after fermentation. Yeast strains, compositions, and methods for reducing DHMB and increasing butanol yield are provided.
摘要翻译： 本发明一般涉及工业微生物学和丁醇生产领域。 更具体地，本发明涉及在丁醇生产中还原2,3-二羟基-2-甲基丁酸酯（DHMB）的方法。 通过抑制乙酰乳酸转化为DHMB可以减少DHMB，例如通过敲除催化还原的酶或在发酵过程中或之后除去DHMB。 提供了用于降低DHMB和提高丁醇产率的酵母菌株，组合物和方法。
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公开(公告)号：US20120064585A1
公开(公告)日：2012-03-15
申请号：US13296944
申请日：2011-11-15
摘要： Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe—S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe—S protein. Activities of heterologous fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe—S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.
摘要翻译： 工程化的酵母菌株具有增加Fe-S簇结合活性的异源蛋白质的活性。 酵母菌株内源性Fe-S蛋白的活性降低。 增加异源真菌或植物2Fe-2S二羟基酸脱水酶和Fe-S丙二醇脱水酶再活化酶的活性，以增加使用包括这些酶的生物合成途径（如缬氨酸，异亮氨酸，亮氨酸，泛酸（维生素B5））， 异丁醇，2-丁酮和2-丁醇。
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公开(公告)号：US20100081179A1
公开(公告)日：2010-04-01
申请号：US12569069
申请日：2009-09-29
摘要： Yeast strains were engineered that have increased activity of heterologous proteins that require binding of an Fe—S cluster for their activity. The yeast strains have reduced activity of an endogenous Fe—S protein. Activities of heterologous fungal or plant 2Fe-2S dihydroxy-acid dehydratases and Fe—S propanediol dehydratase reactivase were increased for increased production of products made using biosynthetic pathways including these enzymes, such as valine, isoleucine, leucine, pantothenic acid (vitamin B5), isobutanol, 2-butanone and 2-butanol.
摘要翻译： 工程化的酵母菌株具有增加Fe-S簇结合活性的异源蛋白质的活性。 酵母菌株内源性Fe-S蛋白的活性降低。 增加异源真菌或植物2Fe-2S二羟基酸脱水酶和Fe-S丙二醇脱水酶再活化酶的活性，以增加使用包括这些酶的生物合成途径（如缬氨酸，异亮氨酸，亮氨酸，泛酸（维生素B5））， 异丁醇，2-丁酮和2-丁醇。
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公开(公告)号：US09297028B2
公开(公告)日：2016-03-29
申请号：US11527995
申请日：2006-09-27
申请人： Gail K. Donaldson , Lixuan Lisa Huang , Lori Ann Maggio-Hall , Vasantha Nagarajan , Charles E. Nakamura , Wonchul Suh
发明人： Gail K. Donaldson , Lixuan Lisa Huang , Lori Ann Maggio-Hall , Vasantha Nagarajan , Charles E. Nakamura , Wonchul Suh
IPC分类号： C12P7/16 , C12N9/10 , C12N9/04 , C12N9/88 , C12N9/02 , C12N9/06 , C12N1/20 , C12N15/00 , C07H21/04
CPC分类号： C12P7/16 , C12N9/0004 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/1029 , C12N9/88 , C12Y101/01157 , C12Y103/99002 , C12Y203/01009 , C12Y402/01055 , Y02E50/10 , Y02P20/52
摘要： Methods for the fermentive production of four carbon alcohols is provided. Specifically, butanol, preferably 1-butanol is produced by the fermentive growth of a recombinant bacterium expressing a 1-butanol biosynthetic pathway.
摘要翻译： 提供了发酵生产四种碳醇的方法。 具体地，通过表达1-丁醇生物合成途径的重组细菌的发酵生长来制备丁醇，优选1-丁醇。
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公开(公告)号：US20080182308A1
公开(公告)日：2008-07-31
申请号：US11527995
申请日：2006-09-27
申请人： Gail K. Donaldson , Lixuan Lisa Huang , Lori Ann Maggio-Hall , Vasantha Nagarajan , Charles E. Nakamura , Wonchul Suh
发明人： Gail K. Donaldson , Lixuan Lisa Huang , Lori Ann Maggio-Hall , Vasantha Nagarajan , Charles E. Nakamura , Wonchul Suh
IPC分类号： C12P7/16
CPC分类号： C12P7/16 , C12N9/0004 , C12N9/0006 , C12N9/0008 , C12N9/001 , C12N9/1029 , C12N9/88 , C12Y101/01157 , C12Y103/99002 , C12Y203/01009 , C12Y402/01055 , Y02E50/10 , Y02P20/52
摘要： Methods for the fermentive production of four carbon alcohols is provided. Specifically, butanol, preferably 1-butanol is produced by the fermentive growth of a recombinant bacterium expressing a 1-butanol biosynthetic pathway.
摘要翻译： 提供了发酵生产四种碳醇的方法。 具体地，通过表达1-丁醇生物合成途径的重组细菌的发酵生长来制备丁醇，优选1-丁醇。
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公开(公告)号：US08669094B2
公开(公告)日：2014-03-11
申请号：US13299954
申请日：2011-11-18
摘要： A high flux in conversion of pyruvate to acetolactate was achieved in yeast through expression of acetolactate synthase in the cytosol in conjunction with reduction in pyruvate decarboxylase activity. Additional manipulations to improve flux to acetolactate are reduced pyruvate dehydrogenase activity and reduced glycerol-3-phosphate dehydrogenase activity. Production of compounds having acetolactate as an upstream intermediate benefit from the increased conversion of pruvate to acetolactate in the described strains.
摘要翻译： 通过在细胞质中表达乙酰乳酸合酶，结合丙酮酸脱羧酶活性的降低，在酵母中实现了丙酮酸向乙酰乳酸转化的高通量。 改善乙酰乳酸通量的额外操作是降低丙酮酸脱氢酶活性和降低甘油-3-磷酸脱氢酶活性。 具有乙酰乳酸酯作为上游中间体的化合物的生产受益于所述菌株中丙酸酯向乙酰乳酸的转化率的增加。
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公开(公告)号：US08956850B2
公开(公告)日：2015-02-17
申请号：US12477942
申请日：2009-06-04
摘要： A high flux in conversion of pyruvate to acetolactate was achieved in yeast through expression of acetolactate synthase in the cytosol in conjunction with reduction in pyruvate decarboxylase activity. Additional manipulations to improve flux to acetolactate are reduced pyruvate dehydrogenase activity and reduced glycerol-3-phosphate dehydrogenase activity. Production of compounds having acetolactate as an upstream intermediate benefit from the increased conversion of pruvate to acetolactate in the described strains.
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公开(公告)号：US20120252084A1
公开(公告)日：2012-10-04
申请号：US13299954
申请日：2011-11-18
摘要： A high flux in conversion of pyruvate to acetolactate was achieved in yeast through expression of acetolactate synthase in the cytosol in conjunction with reduction in pyruvate decarboxylase activity. Additional manipulations to improve flux to acetolactate are reduced pyruvate dehydrogenase activity and reduced glycerol-3-phosphate dehydrogenase activity. Production of compounds having acetolactate as an upstream intermediate benefit from the increased conversion of pruvate to acetolactate in the described strains.
摘要翻译： 通过在细胞质中表达乙酰乳酸合酶，结合丙酮酸脱羧酶活性的降低，在酵母中实现了丙酮酸向乙酰乳酸转化的高通量。 改善乙酰乳酸通量的额外操作是降低丙酮酸脱氢酶活性和降低甘油-3-磷酸脱氢酶活性。 具有乙酰乳酸酯作为上游中间体的化合物的生产受益于所述菌株中丙酸酯向乙酰乳酸的转化率的增加。
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