公开(公告)号：US20170137862A1

公开(公告)日：2017-05-18

申请号：US15345098

申请日：2016-11-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Rouh-Rong JUO ,  Brooks EDWARDS ,  Melissa GEE ,  Zhixian WANG ,  Kathleen SKAARE

IPC: C12Q1/26

CPC classification number: C12Q1/26 ,  C07D305/14 ,  C07D321/00 ,  C07D323/00 ,  C07D407/04 ,  C07D409/04 ,  C07D493/04 ,  C07D493/10 ,  G01N2333/90245 ,  G01N2333/90251 ,  G01N2333/90258

Abstract: Chemiluminescent compositions, methods, assays and kits for oxidative enzymes are described. Further disclosed are dioxetane compounds of the form: where R can independently be any branched alkyl or cycloalkyl group which provides stabilization for the dioxetane or where both R groups together form a cycloalkyl or polycycloalkyl moiety spiro bound to the dioxetane ring, wherein each R group or the spiro bound moiety can be unsubstituted or substituted with one or more electron-withdrawing groups or electron donating groups, or groups providing preferential oxidative isozyme substrate recognition, and wherein R1 is an aryl group, or an alkyl group of 1-20 carbon atoms, which can be optionally substituted with 1 or more halogen atoms, and wherein T is an aryl or heteroaryl ring capable of emitting light upon enzyme activated decomposition of the dioxetane I.Kits, methods and assays are also disclosed that comprise the dioxetane compounds.

公开(公告)号：US20200056222A1

公开(公告)日：2020-02-20

申请号：US16510531

申请日：2019-07-12

Applicant: Life Technologies Corporation

Inventor： Albana MIHALI ,  Brooks EDWARDS ,  Zhixian WANG

IPC: C12Q1/42 ,  G01N33/573 ,  H01L21/02 ,  C07D407/12 ,  C07D321/00

Abstract: Compounds having chemiluminescent flash and glow properties. Also disclosed are methods using the compounds to generate light, detect and/or quantify enzymes, antigens, and/or nucleic acids. Also disclosed are kits relating to these compounds.

公开(公告)号：US20220010356A1

公开(公告)日：2022-01-13

申请号：US17380396

申请日：2021-07-20

Applicant: Life Technologies Corporation

Inventor： Albana MIHALI ,  Brooks EDWARDS ,  Zhixian WANG

IPC: C12Q1/42 ,  C07D321/00 ,  C07D407/12 ,  H01L21/02 ,  G01N33/573

Abstract: Compounds having chemiluminescent flash and glow properties. Also disclosed are methods using the compounds to generate light, detect and/or quantify enzymes, antigens, and/or nucleic acids. Also disclosed are kits relating to these compounds.

公开(公告)号：US20170321245A1

公开(公告)日：2017-11-09

申请号：US15436491

申请日：2017-02-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Albana MIHALI ,  Brooks EDWARDS ,  Zhixian WANG

IPC: C12Q1/42 ,  G01N33/573 ,  C07D407/12 ,  H01L21/02 ,  C07D321/00

CPC classification number: C12Q1/42 ,  C07D321/00 ,  C07D407/12 ,  G01N33/573 ,  H01L21/02491

Abstract: Compounds having chemiluminescent flash and glow properties. Also disclosed are methods using the compounds to generate light, detect and/or quantify enzymes, antigens, and/or nucleic acids. Also disclosed are kits relating to these compounds.