公开(公告)号：US20130292582A1

公开(公告)日：2013-11-07

申请号：US13849456

申请日：2013-03-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Gregory Kaduchak ,  Carleton Stewart ,  Michael D. Ward

IPC: G01N21/64

CPC classification number: G01N15/1404 ,  A61M1/3678 ,  G01N15/12 ,  G01N15/147 ,  G01N21/1702 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/645 ,  G01N27/447 ,  G01N29/222 ,  G01N33/56966 ,  G01N2015/1409 ,  G01N2015/1415 ,  G01N2015/142 ,  G01N2291/02416 ,  G01N2291/02809 ,  G01N2291/02836 ,  Y10T436/101666 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: A method of focusing particles is provided. The method includes transiting a fluid containing particles therein through a channel at a flow rate and adjusting the flow rate for a desired transit time through an interrogation zone through which a light from an excitation source passes. The method further includes optically exciting the particles with the excitation source, detecting an optical signal from the particles, and analyzing the optical signal. The particles may be droplets. Further, the particles may transit the interrogation zone in single file. A system of focusing particles is also provided. The system includes a channel having an inlet for accepting a fluid containing particles. The system further includes a flow adjuster configured to adjust the flow rate for a desired transit time through an interrogation zone, a light source configured to optically excite the particles, and a detector configured to detect optical signals from the particles.

Abstract translation: 提供聚焦颗粒的方法。 该方法包括以流速通过通道流过其中含有颗粒的流体，并通过询问区调整流速以达到期望的通过时间，通过该询问区来自激发源的光通过该询问区。 该方法还包括用激发源光学激发颗粒，检测来自颗粒的光信号，以及分析光信号。 颗粒可以是液滴。 此外，颗粒可以在单个文件中传递询问区。 还提供了聚焦颗粒的系统。 该系统包括具有用于接收含有颗粒的流体的入口的通道。 该系统还包括流量调节器，其被配置为通过询问区域调整所需流通时间的流速，配置为光学激发粒子的光源以及被配置为检测来自粒子的光信号的检测器。

公开(公告)号：US08873051B2

公开(公告)日：2014-10-28

申请号：US13849456

申请日：2013-03-22

Applicant: Life Technologies Corporation

Inventor： Gregory Kaduchak ,  Carleton Stewart ,  Michael D. Ward

IPC: G01N21/00 ,  G01N21/64 ,  G01N29/22 ,  G01N15/14 ,  G01N21/17

CPC classification number: G01N15/1404 ,  A61M1/3678 ,  G01N15/12 ,  G01N15/147 ,  G01N21/1702 ,  G01N21/64 ,  G01N21/6428 ,  G01N21/645 ,  G01N27/447 ,  G01N29/222 ,  G01N33/56966 ,  G01N2015/1409 ,  G01N2015/1415 ,  G01N2015/142 ,  G01N2291/02416 ,  G01N2291/02809 ,  G01N2291/02836 ,  Y10T436/101666 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: A method of focusing particles is provided. The method includes transiting a fluid containing particles therein through a channel at a flow rate and adjusting the flow rate for a desired transit time through an interrogation zone through which a light from an excitation source passes. The method further includes optically exciting the particles with the excitation source, detecting an optical signal from the particles, and analyzing the optical signal. The particles may be droplets. Further, the particles may transit the interrogation zone in single file. A system of focusing particles is also provided. The system includes a channel having an inlet for accepting a fluid containing particles. The system further includes a flow adjuster configured to adjust the flow rate for a desired transit time through an interrogation zone, a light source configured to optically excite the particles, and a detector configured to detect optical signals from the particles.

Abstract translation: 提供聚焦颗粒的方法。 该方法包括以流速通过通道流过其中含有颗粒的流体，并通过询问区调整流速以达到期望的通过时间，通过该询问区来自激发源的光通过该询问区。 该方法还包括用激发源光学激发颗粒，检测来自颗粒的光信号，以及分析光信号。 颗粒可以是液滴。 此外，颗粒可以在单个文件中传递询问区。 还提供了聚焦颗粒的系统。 该系统包括具有用于接收含有颗粒的流体的入口的通道。 该系统还包括流量调节器，其被配置为通过询问区域调整所需流通时间的流速，配置为光学激发粒子的光源以及被配置为检测来自粒子的光信号的检测器。