公开(公告)号：US20150099644A1

公开(公告)日：2015-04-09

申请号：US14395456

申请日：2013-04-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu Chen ,  Casey McFarland ,  David Keys

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q2527/119 ,  C12Q2563/159 ,  C12Q2565/607 ,  C12Q2565/301

Abstract: A method of detection of a target nucleic acid is provided. The method includes fractionating a sample into a plurality of sample volumes wherein more than 50% of the fractions contain no more than 1 target nucleic acid molecule per sample volumes, and subjecting the plurality of sample volumes to conditions for amplification. The method further includes detecting a change in ion concentration in a sample volume wherein a target nucleic acid is present, counting the number of fractions with an amplified target nucleic acid, and determining the quantity of target nucleic acid in the sample.

Abstract translation: 提供了检测靶核酸的方法。 该方法包括将样品分馏成多个样品体积，其中超过50％的级分每个样品体积含有不超过1个靶核酸分子，并且对多个样品体积进行扩增条件。 该方法还包括检测样品体积中的离子浓度的变化，其中存在靶核酸，用扩增的靶核酸计数分数，并测定样品中靶核酸的量。

公开(公告)号：US10318705B2

公开(公告)日：2019-06-11

申请号：US15159717

申请日：2016-05-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Francis T. Cheng ,  Chengyong Yang ,  Casey McFarland ,  Ying Wang

IPC: G01N21/64 ,  G01N25/04 ,  C12Q1/6816 ,  C12Q1/6827 ,  G06F19/22 ,  G06F19/24

Abstract: The present application provides for various embodiments of methods for the analysis of high resolution melt (HRM) curve data; where statistical assay variations in melt curve data may result from system noise in an analysis system. Such system noise may arise from various sources, such as the thermal non-uniformity of a thermocycler block in a thermal cycler apparatus, a detection system, etc. Additionally, various methods for the analysis of HRM curve data may provide an identification of a sample without the need for a user inputted information.

公开(公告)号：US20180327813A1

公开(公告)日：2018-11-15

申请号：US16043002

申请日：2018-07-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu Chen ,  Casey McFarland ,  David Keys

IPC: C12Q1/686 ,  C12Q1/6858 ,  C12Q1/6851

CPC classification number: C12Q1/686 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q2527/119 ,  C12Q2563/159 ,  C12Q2565/607 ,  C12Q2565/301

Abstract: A method of detection of a target nucleic acid is provided. The method includes fractionating a sample into a plurality of sample volumes wherein more than 50% of the fractions contain no more than 1 target nucleic acid molecule per sample volumes, and subjecting the plurality of sample volumes to conditions for amplification. The method further includes detecting a change in ion concentration in a sample volume wherein a target nucleic acid is present, counting the number of fractions with an amplified target nucleic acid, and determining the quantity of target nucleic acid in the sample.

公开(公告)号：US10030262B2

公开(公告)日：2018-07-24

申请号：US14395456

申请日：2013-04-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Caifu Chen ,  Casey McFarland ,  David Keys

IPC: C12P19/34 ,  C12Q1/686 ,  C12Q1/6851 ,  C12Q1/6858

Abstract: A method of detection of a target nucleic acid is provided. The method includes fractionating a sample into a plurality of sample volumes wherein more than 50% of the fractions contain no more than 1 target nucleic acid molecule per sample volumes, and subjecting the plurality of sample volumes to conditions for amplification. The method further includes detecting a change in ion concentration in a sample volume wherein a target nucleic acid is present, counting the number of fractions with an amplified target nucleic acid, and determining the quantity of target nucleic acid in the sample.

公开(公告)号：US20160342734A1

公开(公告)日：2016-11-24

申请号：US15159717

申请日：2016-05-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Francis T. Cheng ,  Chengyong Yang ,  Casey McFarland ,  Ying Wang

IPC: G06F19/22 ,  G01N21/64 ,  C12Q1/68 ,  G01N25/04

CPC classification number: G06F19/22 ,  C12Q1/6816 ,  C12Q1/6827 ,  G01N21/6428 ,  G01N25/04 ,  G01N2021/6439 ,  G06F19/24 ,  C12Q2527/107 ,  C12Q2537/165

Abstract: The present application provides for various embodiments of methods for the analysis of high resolution melt (HRM) curve data; where statistical assay variations in melt curve data may result from system noise in an analysis system. Such system noise may arise from various sources, such as the thermal non-uniformity of a thermocycler block in a thermal cycler apparatus, a detection system, etc. Additionally, various methods for the analysis of HRM curve data may provide an identification of a sample without the need for a user inputted information.