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    Methods and systems for PCR quantitation
    1.
    发明授权
    Methods and systems for PCR quantitation 有权

    公开(公告)号：US10385386B2

    公开(公告)日：2019-08-20

    申请号：US15301947

    申请日：2015-04-03

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Yalei Wu ,  David Keys

    IPC: G16B5/00 ,  C12Q1/6851 ,  G16B30/00 ,  G16B40/00 ,  G16B45/00 ,  G16B25/00 ,  C12Q1/686

    Abstract: A method for quantifying nucleic acid is provided. The method includes determining a first reference threshold cycle for a first predetermined input quantity for a reference nucleic acid, determining a first target threshold cycle for the first predetermined input quantity for a target nucleic acid, determining a second reference threshold cycle for a second predetermined input quantity for the reference nucleic acid, and determining a second target threshold cycle, by the processor, for the second predetermined input quantity for the target nucleic acid. The method further includes receiving a sample threshold cycle, determining a sample input quantity based on the first and second reference threshold cycle and the first and second target threshold cycle, and displaying the sample input quantity to a user.

    Method of performing digital PCR
    2.
    发明授权
    Method of performing digital PCR 有权

    公开(公告)号：US10030262B2

    公开(公告)日：2018-07-24

    申请号：US14395456

    申请日：2013-04-19

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Caifu Chen ,  Casey McFarland ,  David Keys

    IPC: C12P19/34 ,  C12Q1/686 ,  C12Q1/6851 ,  C12Q1/6858

    Abstract: A method of detection of a target nucleic acid is provided. The method includes fractionating a sample into a plurality of sample volumes wherein more than 50% of the fractions contain no more than 1 target nucleic acid molecule per sample volumes, and subjecting the plurality of sample volumes to conditions for amplification. The method further includes detecting a change in ion concentration in a sample volume wherein a target nucleic acid is present, counting the number of fractions with an amplified target nucleic acid, and determining the quantity of target nucleic acid in the sample.

    Methods and Systems for PCR Quantitation
    3.
    发明申请
    Methods and Systems for PCR Quantitation 审中-公开

    公开(公告)号：US20170177791A1

    公开(公告)日：2017-06-22

    申请号：US15301949

    申请日：2015-04-03

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Yalei Wu ,  David Keys

    IPC: G06F19/24 ,  G06F19/22 ,  G06F19/26 ,  G06F19/12

    CPC classification number: C12Q1/6851 ,  C12Q1/686 ,  C12Q2537/16 ,  C12Q2545/114 ,  G16B5/00 ,  G16B25/00 ,  G16B30/00 ,  G16B40/00 ,  G16B45/00

    Abstract: A method for quantifying nucleic acid is provided. The method includes determining a first reference threshold cycle for a first predetermined input quantity for a reference nucleic acid, determining a first target threshold cycle for the first predetermined input quantity for a target nucleic acid, determining a second reference threshold cycle for a second predetermined input quantity for the reference nucleic acid, and determining a second target threshold cycle, by the processor, for the second predetermined input quantity for the target nucleic acid. The method further includes receiving a sample threshold cycle, determining a sample input quantity based on the first and second reference threshold cycle and the first and second target threshold cycle, and displaying the sample input quantity to a user.

    Method of Performing Digital PCR
    4.
    发明申请
    Method of Performing Digital PCR 审中-公开
    执行数字PCR的方法

    公开(公告)号：US20150099644A1

    公开(公告)日：2015-04-09

    申请号：US14395456

    申请日：2013-04-19

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Caifu Chen ,  Casey McFarland ,  David Keys

    IPC: C12Q1/68

    CPC classification number: C12Q1/686 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q2527/119 ,  C12Q2563/159 ,  C12Q2565/607 ,  C12Q2565/301

    Abstract: A method of detection of a target nucleic acid is provided. The method includes fractionating a sample into a plurality of sample volumes wherein more than 50% of the fractions contain no more than 1 target nucleic acid molecule per sample volumes, and subjecting the plurality of sample volumes to conditions for amplification. The method further includes detecting a change in ion concentration in a sample volume wherein a target nucleic acid is present, counting the number of fractions with an amplified target nucleic acid, and determining the quantity of target nucleic acid in the sample.

    Abstract translation: 提供了检测靶核酸的方法。 该方法包括将样品分馏成多个样品体积，其中超过50％的级分每个样品体积含有不超过1个靶核酸分子，并且对多个样品体积进行扩增条件。 该方法还包括检测样品体积中的离子浓度的变化，其中存在靶核酸，用扩增的靶核酸计数分数，并测定样品中靶核酸的量。

    Methods and Systems for PCR Quantitation
    8.
    发明申请
    Methods and Systems for PCR Quantitation 审中-公开

    公开(公告)号：US20180208979A1

    公开(公告)日：2018-07-26

    申请号：US15301947

    申请日：2015-04-03

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Yalei Wu ,  David Keys

    IPC: C12Q1/6851 ,  G06F19/12 ,  G06F19/22 ,  G06F19/24 ,  G06F19/26

    CPC classification number: C12Q1/6851 ,  C12Q1/686 ,  C12Q2537/16 ,  C12Q2545/114 ,  G16B5/00 ,  G16B25/00 ,  G16B30/00 ,  G16B40/00 ,  G16B45/00

    Abstract: A method for quantifying nucleic acid is provided. The method includes determining a first reference threshold cycle for a first predetermined input quantity for a reference nucleic acid, determining a first target threshold cycle for the first predetermined input quantity for a target nucleic acid, determining a second reference threshold cycle for a second predetermined input quantity for the reference nucleic acid, and determining a second target threshold cycle, by the processor, for the second predetermined input quantity for the target nucleic acid. The method further includes receiving a sample threshold cycle, determining a sample input quantity based on the first and second reference threshold cycle and the first and second target threshold cycle, and displaying the sample input quantity to a user.

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