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    Methods and Systems for Image Analysis Identification
    1.
    发明申请
    Methods and Systems for Image Analysis Identification 有权
    图像分析识别方法与系统

    公开(公告)号：US20140247967A1

    公开(公告)日：2014-09-04

    申请号：US14348031

    申请日：2012-09-28

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： David Fortescue ,  Ming Shen ,  Woon Liang Soh ,  Francis T. Cheng

    IPC: G01N35/00 ,  G06T7/00

    CPC classification number: G01N35/00732 ,  G01N35/028 ,  G01N2015/0053 ,  G01N2035/00752 ,  G06T7/0012 ,  G06T2207/10004 ,  G06T2207/30004

    Abstract: A computer-implemented method for identifying a first object-of-interest is provided. The first object-of-interest includes two identifiers and a sample portion. The method includes imaging the first object-of-interest including the two identifiers. The imaging generates a first set of image data. The method further includes determining a position of the first object-of-interest in the field-of-view of an optical sensor and determining the two identifiers from the first set of image data. The method includes identifying the first object-of-interest based on the two identifiers.

    Abstract translation: 提供了一种用于识别第一感兴趣物体的计算机实现的方法。 第一个感兴趣的对象包括两个标识符和一个样本部分。 该方法包括对第一感兴趣物体成像，包括两个标识符。 成像产生第一组图像数据。 该方法还包括确定第一感兴趣物体在光学传感器的视场中的位置，并根据第一组图像数据确定两个标识符。 该方法包括基于两个标识符识别第一感兴趣对象。

    METHODS AND SYSTEMS FOR BACKGROUND SUBTRACTION IN AN IMAGE
    2.
    发明申请
    METHODS AND SYSTEMS FOR BACKGROUND SUBTRACTION IN AN IMAGE 有权
    背景技术在图像中的方法和系统

    公开(公告)号：US20140233816A1

    公开(公告)日：2014-08-21

    申请号：US14347960

    申请日：2012-09-28

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Francis T. Cheng ,  Thomas Wessel

    IPC: G06T7/00

    CPC classification number: G06T7/0012 ,  G06T5/50 ,  G06T7/70 ,  G06T2207/20068 ,  G06T2207/30024 ,  G06T2207/30072

    Abstract: A method for improving image quality is provided. The method includes receiving image data of a substrate, wherein the image data is generated by imaging the substrate, and an image is generated from the image data. The method further includes generating a background representation from a background noise portion of the image, wherein the background portion includes signal information undesired for further processing and generating a background subtracted image by subtracting the background rep resentation from the image. In this way, a separate background image is not needed to subtract the background from the image including the regions-of-interest to improve image quality.

    Abstract translation: 提供了一种提高图像质量的方法。 该方法包括接收基板的图像数据，其中通过对基板进行成像而生成图像数据，并且从图像数据生成图像。 所述方法还包括从所述图像的背景噪声部分生成背景表示，其中所述背景部分包括不期望用于进一步处理的信号信息，并且通过从所述图像中减去所述背景重现来生成背景减去图像。 以这种方式，不需要单独的背景图像从包括感兴趣区域的图像中减去背景以提高图像质量。

    Methods for the analysis of dissociation melt curve data
    3.
    发明授权
    Methods for the analysis of dissociation melt curve data 有权

    公开(公告)号：US10176277B2

    公开(公告)日：2019-01-08

    申请号：US14011190

    申请日：2013-08-27

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Francis T. Cheng ,  Casey R. McFarland

    IPC: G01K15/00 ,  G01K19/00 ,  G06F17/50 ,  C12Q1/6816 ,  G06F19/18

    Abstract: Methods are provided that operate on raw dissociation data and dissociation curves to generate calibrations of the detected data and to further improve analysis of the data. The data can be taken from each support region of a multi-region platform, for example, from each well of a multi-well plate. Each support region can be loaded with portions of the same sample. In some embodiments, a dissociation curve correction can be calibrated for the sample, prior to a run of an experiment using such sample. In some embodiments, a method is provided for generating a melting transition region of dissociation curves that show the melting characteristics of the sample. In some embodiments, dye temperature dependence correction can be performed on the dissociation curve data to further improve analysis. In some embodiments, a feature vector can be derived from the melt data, and the feature vector can be used to further improve genotyping analysis of the dissociation curves.

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