公开(公告)号：US20180230516A1

公开(公告)日：2018-08-16

申请号：US15950921

申请日：2018-04-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jian GU ,  Kelli BRAMLETT ,  Christopher BURNETT

IPC: C12Q1/6806 ,  C12Q1/6848

CPC classification number: C12Q1/6806 ,  C12Q1/6848 ,  C12Q2521/501 ,  C12Q2525/113 ,  C12Q2525/186 ,  C12Q2525/191 ,  C12Q2537/163

Abstract: Disclosed are compositions and methods for the preparation of RNA libraries for sequencing, gene expression profiling, microarray and other uses and for simplification of the library preparation process. The disclosure provides blocking oligonucleotides which bind to byproduct nucleic acid molecules formed during the ligation of adapters to nucleic acid segments prior to sequencing and inhibit or block amplification of the byproduct nucleic acid molecules in subsequent amplification reactions. Methods for library preparation using blocking oligonucleotides are also provided.

公开(公告)号：US20180057814A1

公开(公告)日：2018-03-01

申请号：US15691721

申请日：2017-08-30

Applicant: Life Technologies Corporation

Inventor： Christopher BURNETT ,  Nitin Puri ,  Susan Magdaleno ,  Alexander Vlassov

IPC: C12N15/113 ,  A61K31/713 ,  C12N15/11

CPC classification number: C12N15/113 ,  A61K31/713 ,  A61K47/50 ,  C12N15/111 ,  C12N2310/113 ,  C12N2310/141 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/3231 ,  C12N2310/332 ,  C12N2310/3515 ,  C12N2310/3519 ,  C12N2310/51 ,  C12N2310/531 ,  C12N2330/31

Abstract: The invention relates generally to compositions and methods for inhibiting the function of target nucleic acids by sequence specific binding. The compositions and methods can be used for inhibition of micro RNAs and other relatively short non-coding RNAs.

公开(公告)号：US20170009232A1

公开(公告)日：2017-01-12

申请号：US15217007

申请日：2016-07-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Christopher BURNETT ,  Nitin Puri ,  Susan Magdaleno ,  Alexander Vlassov

IPC: C12N15/113 ,  A61K47/48 ,  A61K31/713

CPC classification number: C12N15/113 ,  A61K31/713 ,  A61K47/50 ,  C12N15/111 ,  C12N2310/113 ,  C12N2310/141 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/3231 ,  C12N2310/332 ,  C12N2310/3515 ,  C12N2310/3519 ,  C12N2310/51 ,  C12N2310/531 ,  C12N2330/31

Abstract: The invention relates generally to compositions and methods for inhibiting the function of target nucleic acids by sequence specific binding. The compositions and methods can be used for inhibition of micro RNAs and other relatively short non-coding RNAs.

Abstract translation: 本发明一般涉及通过序列特异性结合抑制靶核酸功能的组合物和方法。 组合物和方法可用于抑制微RNA和其他相对较短的非编码RNA。