公开(公告)号：US20150037791A1

公开(公告)日：2015-02-05

申请号：US14328074

申请日：2014-07-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Lori HENNESSY ,  Dennis WANG

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/6876 ,  C12Q2600/16 ,  C12Q2565/137 ,  C12Q2565/102 ,  C12Q2537/143 ,  C12Q2565/125

Abstract: Compositions, methods and kits are disclosed for use in simultaneously amplifying at least 20 specific STR loci of genomic nucleic acid in a single multiplex reaction, as are methods and materials for use in the analysis of the products of such reactions. Included in the present invention are materials and methods for the simultaneous amplification of 23 and 24 specific loci in a single multiplex reaction, comprising the 13 CODIS loci, the Amelogenin locus, an InDel and at least six to ten additional STR loci, including methods, kits and materials for the analysis of these loci.

Abstract translation: 公开了用于在单个多重反应中同时扩增基因组核酸的至少20个特异性STR基因座的组合物，方法和试剂盒，以及用于分析这些反应的产物的方法和材料。 本发明包括用于在单个多重反应中同时扩增23和24个特异性基因座的材料和方法，包括13个CODIS基因座，Amelogenin基因座，InDel和至少6至10个另外的STR基因座，包括方法， 用于分析这些基因座的试剂盒和材料。

公开(公告)号：US20230099446A1

公开(公告)日：2023-03-30

申请号：US17950990

申请日：2022-09-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Dennis WANG ,  Lori HENNESSY

IPC: C12Q1/6858 ,  C12Q1/686

Abstract: Disclosed is a method of amplifying a nucleic acid sequence, wherein the method comprises subjecting a reaction mixture to at least one amplification cycle, wherein the reaction mixture comprises a double-stranded nucleic acid and at least two primers capable of annealing to complementary strands of the double-stranded nucleic acid and amplifying at least one short tandem repeat (STR) using a Family A DNA polymerase in a Fast PCR protocol having a two-step amplification cycle in 25 seconds or less. Also disclosed are real-time PCR methods using the two-step protocol and kits for STR profiling using the Fast PCR protocol.

公开(公告)号：US20220205023A1

公开(公告)日：2022-06-30

申请号：US17585161

申请日：2022-01-26

Applicant: Life Technologies Corporation

Inventor： Robert GREEN ,  Dennis WANG ,  Julio MULERO ,  Robert LAGACE

IPC: C12Q1/6827 ,  C12Q1/6888

Abstract: Disclosed are primer set compositions, methods and kits for human identification using the highly complex sequence locus, SE33 (ACTBP2) in single and multiplex PCR reactions. Additionally, disclosed are three newly discovered single nucleotide polymorphisms (SNPs) within the SE33 locus that can cause discordance seen as mobility shift or allelic dropout. Also disclosed are kits useful in human identification.

公开(公告)号：US20150354015A1

公开(公告)日：2015-12-10

申请号：US14739722

申请日：2015-06-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Chien-Wei CHANG ,  Lori HENNESSY ,  Dennis WANG

IPC: C12Q1/68

CPC classification number: C12Q1/6888 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/686 ,  C12Q2527/125

Abstract: The present teachings relate to improved methods, kits, and reaction mixtures for amplifying nucleic acids. In some embodiments a novel direct buffer formulation is provided which allows for the direct amplification of the nucleic acids in a crude sample with minimal sample purification.

Abstract translation: 本教导涉及用于扩增核酸的改进的方法，试剂盒和反应混合物。 在一些实施方案中，提供了新的直接缓冲液制剂，其允许以最少的样品纯化直接扩增粗样品中的核酸。