公开(公告)号：US20180030534A1

公开(公告)日：2018-02-01

申请号：US15730536

申请日：2017-10-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason MYERS ,  Zhoutao CHEN ,  Devin DRESSMAN ,  Theo NIKIFOROV

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2521/307 ,  C12Q2521/325 ,  C12Q2525/125 ,  C12Q2535/119 ,  C12Q2565/518

Abstract: In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, In some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.

公开(公告)号：US20170145500A1

公开(公告)日：2017-05-25

申请号：US15423744

申请日：2017-02-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason MYERS ,  Zhoutao CHEN ,  Devin DRESSMAN ,  Theo NIKIFOROV

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2521/307 ,  C12Q2521/325 ,  C12Q2525/125 ,  C12Q2535/119 ,  C12Q2565/518

Abstract: In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, In some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.

公开(公告)号：US20150353994A1

公开(公告)日：2015-12-10

申请号：US14832772

申请日：2015-08-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason MYERS ,  Zhoutao CHEN ,  Devin DRESSMAN ,  Theo NIKIFOROV

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2521/307 ,  C12Q2521/325 ,  C12Q2525/125 ,  C12Q2535/119 ,  C12Q2565/518

Abstract: In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, In some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.

Abstract translation: 在一些实施方案中，从与支持物连接的核酸模板获得序列信息的方法包括将第一引物与连接于载体的模板链杂交，对核酸模板的一部分进行测序，从而形成延伸的第一引物产物， 与一部分核酸模板互补。在一些实施方案中，该方法还包括将切口引入与延伸的第一引物产物杂交的模板链的一部分中，使用下述方法从模板中降解模板链的一部分： 其中一部分延伸的第一引物保留与模板链的未降解部分杂交，并通过合成测序延伸的第一引物的至少一些单链部分。