公开(公告)号：US20180030534A1

公开(公告)日：2018-02-01

申请号：US15730536

申请日：2017-10-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason MYERS ,  Zhoutao CHEN ,  Devin DRESSMAN ,  Theo NIKIFOROV

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2521/307 ,  C12Q2521/325 ,  C12Q2525/125 ,  C12Q2535/119 ,  C12Q2565/518

Abstract: In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, In some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.

公开(公告)号：US20140343265A1

公开(公告)日：2014-11-20

申请号：US14315140

申请日：2014-06-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Zhoutao CHEN ,  Xiaoping DUAN ,  Kyusung PARK

IPC: C12P19/40

CPC classification number: C12P19/40 ,  C12N15/10 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q2521/301 ,  C12Q2521/101 ,  C12Q2521/307 ,  C12Q2521/501 ,  C12Q2525/191

Abstract: In some embodiments, the present teachings provide compositions, systems, methods and kits for generating a population of nucleic acid fragments. In some embodiments, nucleic acids can be fragmented enzymatically. For example, methods for generating a population of nucleic acid fragments can include a nucleic acid nicking reaction. In one embodiment, the methods can include a nick translation reaction. A nicking reaction can introduce nicks at random positions on either strand of a double-stranded nucleic acid. A nick translation reaction can move the position of nicks to a new position so that the new positions of two of the nicks are aligned to create a double-stranded break. In some embodiments, methods for generating a population of nucleic acid fragments can include joining at least one end of a fragmented nucleic acid to one or more oligonucleotide adaptors.

Abstract translation: 在一些实施方案中，本教导提供了用于产生核酸片段群体的组合物，系统，方法和试剂盒。 在一些实施方案中，核酸可以酶促碎裂。 例如，产生核酸片段群的方法可以包括核酸切口反应。 在一个实施方案中，所述方法可以包括切口平移反应。 切口反应可以在双链核酸的任一链上的随机位置引入缺口。 切口平移反应可以将切口的位置移动到新位置，使得两个切口的新位置对齐以产生双链断裂。 在一些实施方案中，用于产生核酸片段群的方法可包括将片段化核酸的至少一端连接至一个或多个寡核苷酸衔接子。

公开(公告)号：US20230340554A1

公开(公告)日：2023-10-26

申请号：US18081582

申请日：2022-12-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Zhoutao CHEN ,  Xiaoping DUAN ,  Kyusung PARK

IPC: C12P19/40 ,  C12N15/10 ,  C12Q1/6806

CPC classification number: C12P19/40 ,  C12N15/1093 ,  C12Q1/6806 ,  C12N15/10

Abstract: In some embodiments, the present teachings provide compositions, systems, methods and kits for generating a population of nucleic acid fragments. In some embodiments, nucleic acids can be fragmented enzymatically. For example, methods for generating a population of nucleic acid fragments can include a nucleic acid nicking reaction. In one embodiment, the methods can include a nick translation reaction. A nicking reaction can introduce nicks at random positions on either strand of a double-stranded nucleic acid. A nick translation reaction can move the position of nicks to a new position so that the new positions of two of the nicks are aligned to create a double-stranded break. In some embodiments, methods for generating a population of nucleic acid fragments can include joining at least one end of a fragmented nucleic acid to one or more oligonucleotide adaptors.

公开(公告)号：US20170145500A1

公开(公告)日：2017-05-25

申请号：US15423744

申请日：2017-02-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason MYERS ,  Zhoutao CHEN ,  Devin DRESSMAN ,  Theo NIKIFOROV

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2521/307 ,  C12Q2521/325 ,  C12Q2525/125 ,  C12Q2535/119 ,  C12Q2565/518

Abstract: In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, In some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.

公开(公告)号：US20170088893A1

公开(公告)日：2017-03-30

申请号：US15372938

申请日：2016-12-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Bin LI ,  Zhoutao CHEN ,  Tanya BIORAC ,  Melvin WEI

IPC: C12Q1/68 ,  C12N9/20 ,  C12N9/12

CPC classification number: C12Q1/6876 ,  C12N9/1252 ,  C12N9/20 ,  C12N15/1093 ,  C12N15/64 ,  C12Q1/6806 ,  C12Q1/6832 ,  C12Y207/07007 ,  C12Y301/01003 ,  C12Q2521/131 ,  C12Q2525/191 ,  C12Q2527/107 ,  C12Q2537/163 ,  C12Q2563/179

Abstract: Provided herein are compositions, systems, methods, and kits for joining together the ends of one or more polynucleotides using at least one pair of blocking oligonucleotide adaptors. Blocking oligonucleotide adaptors can be used to reduce the formation of adaptor dimers or trimers (or higher-order concatemers) which can improve the yield of desirable polynucleotide-adaptor products in any recombinant nucleic acid workflow. Blocking oligonucleotide adaptors can comprise a double-stranded oligonucleotide adaptor (duplex) having an overhang cohesive portion that anneals with a blocking oligonucleotide which can be a separate single-stranded oligonucleotide. A blocking oligonucleotide, when annealed to an overhang portion, can prevent undesirable hybridization of the overhang portion to another nucleic acid, such as the overhang portion from another blocking oligonucleotide adaptor or a polynucleotide of interest.

公开(公告)号：US20150353994A1

公开(公告)日：2015-12-10

申请号：US14832772

申请日：2015-08-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jason MYERS ,  Zhoutao CHEN ,  Devin DRESSMAN ,  Theo NIKIFOROV

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q2521/307 ,  C12Q2521/325 ,  C12Q2525/125 ,  C12Q2535/119 ,  C12Q2565/518

Abstract: In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, In some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis.

Abstract translation: 在一些实施方案中，从与支持物连接的核酸模板获得序列信息的方法包括将第一引物与连接于载体的模板链杂交，对核酸模板的一部分进行测序，从而形成延伸的第一引物产物， 与一部分核酸模板互补。在一些实施方案中，该方法还包括将切口引入与延伸的第一引物产物杂交的模板链的一部分中，使用下述方法从模板中降解模板链的一部分： 其中一部分延伸的第一引物保留与模板链的未降解部分杂交，并通过合成测序延伸的第一引物的至少一些单链部分。