公开(公告)号：US20160251664A1

公开(公告)日：2016-09-01

申请号：US15060536

申请日：2016-03-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan Chesnut ,  John Carrino ,  Louis Leong ,  Knut Madden ,  Matin Gleeson ,  James Fan ,  Michael Brasch ,  David Cheo ,  James Hartley ,  Devon Byrd ,  Gary Temple

IPC: C12N15/66 ,  C12P19/34

CPC classification number: C12N15/66 ,  C12N9/90 ,  C12N15/10 ,  C12N15/64 ,  C12N15/902 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2800/70 ,  C12N2840/20 ,  C12P19/34 ,  C12Y599/01

Abstract: The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. The invention also provides host cells comprising nucleic acid molecules of the invention or prepared according to the methods of the invention, and also provides kits comprising the compositions, host cells and nucleic acid molecules of the invention, which may be used to synthesize nucleic acid molecules according to the methods of the invention.

公开(公告)号：US08945884B2

公开(公告)日：2015-02-03

申请号：US13892903

申请日：2013-05-13

Applicant: Life Technologies Corporation

Inventor： Jonathan Chesnut ,  John Carrino ,  Louis Leong ,  Knut Madden ,  Martin Gleeson ,  James Fan ,  Michael Brasch ,  David Cheo ,  James Hartley ,  Devon Byrd ,  Gary Temple

IPC: C12N15/64 ,  C12P19/34 ,  C12N15/10 ,  C12N15/66 ,  C12N15/90

CPC classification number: C12N15/66 ,  C12N9/90 ,  C12N15/10 ,  C12N15/64 ,  C12N15/902 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2800/70 ,  C12N2840/20 ,  C12P19/34 ,  C12Y599/01

Abstract: The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. The invention also provides host cells comprising nucleic acid molecules of the invention or prepared according to the methods of the invention, and also provides kits comprising the compositions, host cells and nucleic acid molecules of the invention, which may be used to synthesize nucleic acid molecules according to the methods of the invention.

Abstract translation: 本发明提供了用于重组克隆的组合物和方法。 组合物包括具有多个重组位点和/或多个拓扑异构酶识别位点的载体。 该方法允许同时克隆两种或更多种不同的核酸分子。 在一些实施方案中，分子融合在一起，而在其它实施方案中，分子被插入载体中的不同位点。 本发明还通常提供通过重组连接或连接许多可以相同或不同的分子和/或化合物（例如，化合物，药物，蛋白质或肽，脂质，核酸，碳水化合物等）。 本发明还提供了包含本发明的核酸分子或根据本发明的方法制备的宿主细胞，还提供了包含本发明的组合物，宿主细胞和核酸分子的试剂盒，其可用于合成核酸分子 根据本发明的方法。

公开(公告)号：US20160264962A1

公开(公告)日：2016-09-15

申请号：US14811363

申请日：2015-07-28

Applicant: Life Technologies Corporation ,  GeneArt Ag

Inventor： Federico KATZEN ,  Jonathan Chesnut ,  Frank Notka ,  Matthias Arnold ,  Kevin Hoff ,  Stephen Whiteside

IPC: C12N15/10 ,  C12N9/90 ,  C07K14/195 ,  C12N9/96

CPC classification number: C12N15/1082 ,  C07K14/195 ,  C12N9/90 ,  C12N9/96 ,  C12N15/102 ,  C12N15/1086 ,  C12N15/1093 ,  C12N15/66 ,  C12Y599/01002

Abstract: The invention generally relates to compositions and methods for designing and producing functional DNA binding effector molecules and associated customized services, tool kits and functional assays. In some aspects, the invention provides methods and tools for efficient assembly of customized TAL effector molecules. Furthermore, the invention relates to uses of TAL effector molecules and functional evaluation of such TAL by, for example, customized assays.

公开(公告)号：US20130274129A1

公开(公告)日：2013-10-17

申请号：US13856978

申请日：2013-04-04

Applicant: GENEART AG ,  LIFE TECHNOLOGIES CORPORATION

Inventor： Federico KATZEN ,  Jonathan Chesnut ,  Frank Notka ,  Matthias Arnold ,  Kevin Hoff ,  Stephen Whiteside

IPC: C07K14/195 ,  C12N15/66 ,  C12N15/10

CPC classification number: C12N15/1082 ,  C07K14/195 ,  C12N9/90 ,  C12N9/96 ,  C12N15/102 ,  C12N15/1086 ,  C12N15/1093 ,  C12N15/66 ,  C12Y599/01002

Abstract: The invention generally relates to compositions and methods for designing and producing functional DNA binding effector molecules and associated customized services, tool kits and functional assays. In some aspects, the invention provides methods and tools for efficient assembly of customized TAL effector molecules. Furthermore, the invention relates to uses of TAL effector molecules and functional evaluation of such TAL by, for example, customized assays.

Abstract translation: 本发明一般涉及用于设计和产生功能性DNA结合效应分子和相关定制服务，工具包和功能测定的组合物和方法。 在一些方面，本发明提供了用于有效组装定制的TAL效应分子的方法和工具。 此外，本发明涉及TAL效应分子的用途和通过例如定制测定法对这种TAL的功能评估。

公开(公告)号：US09309520B2

公开(公告)日：2016-04-12

申请号：US14578336

申请日：2014-12-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan Chesnut ,  John Carrino ,  Louis Leong ,  Knut Madden ,  Martin Gleeson ,  James Fan ,  Michael Brasch ,  David Cheo ,  James Hartley ,  Devon Byrd ,  Gary Temple

IPC: C12N15/70 ,  C12N15/66 ,  C12N15/10 ,  C12N15/64 ,  C12N15/90 ,  C12P19/34 ,  C12N9/90

CPC classification number: C12N15/66 ,  C12N9/90 ,  C12N15/10 ,  C12N15/64 ,  C12N15/902 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2800/70 ,  C12N2840/20 ,  C12P19/34 ,  C12Y599/01

Abstract: The present invention provides compositions and methods for recombinational cloning. The compositions include vectors having multiple recombination sites and/or multiple topoisomerase recognition sites. The methods permit the simultaneous cloning of two or more different nucleic acid molecules. In some embodiments the molecules are fused together while in other embodiments the molecules are inserted into distinct sites in a vector. The invention also generally provides for linking or joining through recombination a number of molecules and/or compounds (e.g., chemical compounds, drugs, proteins or peptides, lipids, nucleic acids, carbohydrates, etc.) which may be the same or different. The invention also provides host cells comprising nucleic acid molecules of the invention or prepared according to the methods of the invention, and also provides kits comprising the compositions, host cells and nucleic acid molecules of the invention, which may be used to synthesize nucleic acid molecules according to the methods of the invention.

公开(公告)号：US20160010113A1

公开(公告)日：2016-01-14

申请号：US14801757

申请日：2015-07-16

Applicant: Life Technologies Corporation

Inventor： Uma LAKSHMIPATHY ,  Bhaskar Thyagarajan ,  Jonathan Chesnut ,  Vasiliki Anest ,  Robert Bennett ,  Pauline Lieu ,  George Hanson ,  David Thompson ,  Lucas Chase ,  Gary Shipley ,  Elizabeth Wilson

IPC: C12N15/86

CPC classification number: C12N15/86 ,  C12N2710/16221 ,  C12N2710/16222 ,  C12N2710/16243 ,  C12N2799/022 ,  C12N2799/026 ,  C12N2799/027 ,  C12N2800/108 ,  C12N2820/002 ,  C12N2820/007 ,  C12N2820/60

Abstract: The disclosure relates generally to genetic manipulation of stem and primary cells and to reprogramming of somatic cells, more specifically, human cells. In particular, compositions and methods are disclosed for the generation and maintenance of such engineered cells.

Abstract translation: 本公开一般涉及干细胞和原代细胞的遗传操作和体细胞，更具体地，人细胞的重编程。 特别地，公开了用于产生和维护这种工程化细胞的组合物和方法。