公开(公告)号：US11464863B2

公开(公告)日：2022-10-11

申请号：US16557967

申请日：2019-08-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Zhiwei Yang ,  Parul Angrish ,  Xavier de Mollerat du Jeu ,  Kristin Wiederholt

IPC: A61K47/54 ,  C07C227/06 ,  C07D211/28 ,  C07D211/58 ,  C12N15/88 ,  A61K48/00 ,  C07C229/08 ,  C12N15/113

Abstract: There are provided for herein novel amine-containing transfection compounds and methods for making and using same. The compounds are generally obtained by reacting a primary amine with an unsaturated compound. Transfection complexes made using the amine-containing transfection compounds in combination with additional compounds to encapsulate biologically active agents such as nucleic acids are also provided for herein. Methods of using the transfection complexes for the in vivo or in vitro delivery of biologically active agents are also described. The transfection complexes of the present invention are highly potent, thereby allowing effective modulation of a biological activity at relatively low doses compared to analogous transfection compounds known in the art.

公开(公告)号：US20190002880A1

公开(公告)日：2019-01-03

申请号：US16131512

申请日：2018-09-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Tod Woolf ,  Kristin Wiederholt

IPC: C12N15/113 ,  C12N15/11 ,  A61K31/713 ,  C12N13/00

Abstract: Antisense sequences, including duplex RNAi compositions, which possess improved properties over those taught in the prior art are disclosed. The invention provides optimized antisense oligomer compositions and method for making and using the both in in vitro systems and therapeutically. The invention also provides methods of making and using the improved antisense oligomer compositions.

公开(公告)号：US20150174260A1

公开(公告)日：2015-06-25

申请号：US14340395

申请日：2014-07-24

Applicant: Life Technologies Corporation

Inventor： Zhiwei Yang ,  Parul Angrish ,  Xavier de Mollerat du Jeu ,  Kristin Wiederholt ,  Tao Wu

IPC: A61K47/48 ,  C12N15/113 ,  A61K48/00 ,  C07C229/08

CPC classification number: A61K47/54 ,  A61K47/543 ,  A61K48/0008 ,  C07C227/06 ,  C07C229/08 ,  C07D211/28 ,  C07D211/58 ,  C12N15/1137 ,  C12N15/88 ,  C12N2310/11 ,  C12N2310/141 ,  C07C229/26

Abstract: There are provided for herein novel amine-containing transfection compounds and methods for making and using same. The compounds are generally obtained by reacting a primary amine with an unsaturated compound. Transfection complexes made using the amine-containing transfection compounds in combination with additional compounds to encapsulate biologically active agents such as nucleic acids are also provided for herein. Methods of using the transfection complexes for the in vivo or in vitro delivery of biologically active agents are also described. The transfection complexes of the present invention are highly potent, thereby allowing effective modulation of a biological activity at relatively low doses compared to analogous transfection compounds known in the art.

Abstract translation: 本文提供了新颖的含胺转染化合物及其制备和使用方法。 化合物通常通过使伯胺与不饱和化合物反应而获得。 本文还提供了使用含胺转染化合物与附加化合物组合以包封生物活性剂如核酸制备的转染复合物。 还描述了使用转染复合物用于体内或体外递送生物活性剂的方法。 与本领域已知的类似转染化合物相比，本发明的转染复合物是非常有效的，从而允许相对低剂量的生物活性的有效调节。

公开(公告)号：US20230181742A1

公开(公告)日：2023-06-15

申请号：US17821122

申请日：2022-08-19

Applicant: Life Technologies Corporation

Inventor： Zhiwei Yang ,  Parul Angrish ,  Xavier de Mollerat Du Jeu ,  Kristin Wiederholt

IPC: A61K47/54 ,  C07C227/06 ,  C07D211/28 ,  C07D211/58 ,  C12N15/88 ,  A61K48/00 ,  C07C229/08 ,  C12N15/113

CPC classification number: A61K47/54 ,  C07C227/06 ,  C07D211/28 ,  C07D211/58 ,  C12N15/88 ,  A61K48/0008 ,  C07C229/08 ,  C12N15/1137 ,  A61K47/543 ,  C12N2310/11 ,  C12N2310/141

Abstract: There are provided for herein novel amine-containing transfection compounds and methods for making and using same. The compounds are generally obtained by reacting a primary amine with an unsaturated compound. Transfection complexes made using the amine-containing transfection compounds in combination with additional compounds to encapsulate biologically active agents such as nucleic acids are also provided for herein. Methods of using the transfection complexes for the in vivo or in vitro delivery of biologically active agents are also described. The transfection complexes of the present invention are highly potent, thereby allowing effective modulation of a biological activity at relatively low doses compared to analogous transfection compounds known in the art.

公开(公告)号：US20170137816A1

公开(公告)日：2017-05-18

申请号：US15418653

申请日：2017-01-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Tod WOOLF ,  Kristin Wiederholt

IPC: C12N15/113 ,  C12N13/00

CPC classification number: C12N15/113 ,  A61K31/713 ,  C12N13/00 ,  C12N15/111 ,  C12N15/1135 ,  C12N15/1137 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/33 ,  C12N2310/351 ,  C12N2310/53 ,  C12N2320/31 ,  C12N2320/50 ,  C12N2320/51 ,  C12Y207/11022 ,  C12Y301/03048

Abstract: Antisense sequences, including duplex RNAi compositions, which possess improved properties over those taught in the prior art are disclosed. The invention provides optimized antisense oligomer compositions and method for making and using the both in in vitro systems and therapeutically. The invention also provides methods of making and using the improved antisense oligomer compositions.

公开(公告)号：US09592250B2

公开(公告)日：2017-03-14

申请号：US14446022

申请日：2014-07-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Tod Woolf ,  Kristin Wiederholt

IPC: C12N15/11 ,  A61K31/713 ,  C12N15/113

CPC classification number: C12N15/113 ,  A61K31/713 ,  C12N13/00 ,  C12N15/111 ,  C12N15/1135 ,  C12N15/1137 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/33 ,  C12N2310/351 ,  C12N2310/53 ,  C12N2320/31 ,  C12N2320/50 ,  C12N2320/51 ,  C12Y207/11022 ,  C12Y301/03048

Abstract: Antisense sequences, including duplex RNAi compositions, which possess improved properties over those taught in the prior art are disclosed. The invention provides optimized antisense oligomer compositions and method for making and using the both in in vitro systems and therapeutically. The invention also provides methods of making and using the improved antisense oligomer compositions.

Abstract translation: 公开了具有比现有技术中教导的那些改进的性能的反义序列，包括双链RNAi组合物。 本发明提供了在体外系统和治疗上制备和使用这两者的优化的反义寡聚体组合物和方法。 本发明还提供了制备和使用改进的反义寡聚体组合物的方法。

公开(公告)号：US10406237B2

公开(公告)日：2019-09-10

申请号：US15844380

申请日：2017-12-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Zhiwei Yang ,  Parul Angrish ,  Xavier de Mollerat du Jeu ,  Kristin Wiederholt

IPC: A61K47/54 ,  C07C227/06 ,  C07D211/28 ,  C07D211/58 ,  C12N15/88 ,  A61K48/00 ,  C07C229/08 ,  C12N15/113

Abstract: There are provided for herein novel amine-containing transfection compounds and methods for making and using same. The compounds are generally obtained by reacting a primary amine with an unsaturated compound. Transfection complexes made using the amine-containing transfection compounds in combination with additional compounds to encapsulate biologically active agents such as nucleic acids are also provided for herein. Methods of using the transfection complexes for the in vivo or in vitro delivery of biologically active agents are also described. The transfection complexes of the present invention are highly potent, thereby allowing effective modulation of a biological activity at relatively low doses compared to analogous transfection compounds known in the art.

公开(公告)号：US10106793B2

公开(公告)日：2018-10-23

申请号：US15418653

申请日：2017-01-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Tod Woolf ,  Kristin Wiederholt

IPC: C12N15/113 ,  C12N13/00

Abstract: Antisense sequences, including duplex RNAi compositions, which possess improved properties over those taught in the prior art are disclosed. The invention provides optimized antisense oligomer compositions and method for making and using the both in in vitro systems and therapeutically. The invention also provides methods of making and using the improved antisense oligomer compositions.

公开(公告)号：US09901642B2

公开(公告)日：2018-02-27

申请号：US14340395

申请日：2014-07-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Zhiwei Yang ,  Parul Angrish ,  Xavier de Mollerat du Jeu ,  Kristin Wiederholt ,  Tao Wu

IPC: C07C227/06 ,  C07D211/28 ,  C07D211/58 ,  C12N15/88 ,  A61K48/00 ,  C07C229/08 ,  C12N15/113 ,  A61K47/54 ,  A61K47/48

CPC classification number: A61K47/54 ,  A61K47/543 ,  A61K48/0008 ,  C07C227/06 ,  C07C229/08 ,  C07D211/28 ,  C07D211/58 ,  C12N15/1137 ,  C12N15/88 ,  C12N2310/11 ,  C12N2310/141 ,  C07C229/26

Abstract: There are provided for herein novel amine-containing transfection compounds and methods for making and using same. The compounds are generally obtained by reacting a primary amine with an unsaturated compound. Transfection complexes made using the amine-containing transfection compounds in combination with additional compounds to encapsulate biologically active agents such as nucleic acids are also provided for herein. Methods of using the transfection complexes for the in vivo or in vitro delivery of biologically active agents are also described. The transfection complexes of the present invention are highly potent, thereby allowing effective modulation of a biological activity at relatively low doses compared to analogous transfection compounds known in the art.