公开(公告)号：US11464863B2

公开(公告)日：2022-10-11

申请号：US16557967

申请日：2019-08-30

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Zhiwei Yang ,  Parul Angrish ,  Xavier de Mollerat du Jeu ,  Kristin Wiederholt

IPC: A61K47/54 ,  C07C227/06 ,  C07D211/28 ,  C07D211/58 ,  C12N15/88 ,  A61K48/00 ,  C07C229/08 ,  C12N15/113

Abstract: There are provided for herein novel amine-containing transfection compounds and methods for making and using same. The compounds are generally obtained by reacting a primary amine with an unsaturated compound. Transfection complexes made using the amine-containing transfection compounds in combination with additional compounds to encapsulate biologically active agents such as nucleic acids are also provided for herein. Methods of using the transfection complexes for the in vivo or in vitro delivery of biologically active agents are also described. The transfection complexes of the present invention are highly potent, thereby allowing effective modulation of a biological activity at relatively low doses compared to analogous transfection compounds known in the art.

公开(公告)号：US20230100065A1

公开(公告)日：2023-03-30

申请号：US17961394

申请日：2022-10-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Parul Angrish ,  Zhiwei Yang

IPC: C12Q1/686 ,  C07C41/22 ,  C07C211/63 ,  C07C303/28 ,  C12N9/96 ,  C07D211/60 ,  C11D1/88 ,  C12N9/12 ,  C07D207/16

Abstract: This disclosure relates to novel compounds for use in various compositions, kits and methods, including, for example, use in polymerase storage buffers and in nucleic acid synthesis or amplification reactions such as a polymerase chain reaction (PCR). Methods for preparing the novel compounds are also described.

公开(公告)号：US11697841B2

公开(公告)日：2023-07-11

申请号：US16870398

申请日：2020-05-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Parul Angrish ,  Zhiwei Yang ,  Jonathan Wang

IPC: C12Q1/68 ,  C12Q1/6848 ,  C07C229/12 ,  C07C229/24 ,  C07D207/16 ,  C12Q1/6844 ,  C07D211/60 ,  C12Q1/6806 ,  C12Q1/6876

CPC classification number: C12Q1/6848 ,  C07C229/12 ,  C07C229/24 ,  C07D207/16 ,  C07D211/60 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6876 ,  C12Q2600/158 ,  C12Q1/6844 ,  C12Q2527/125

Abstract: This disclosure relates to novel detergents for use in various procedures including, for example, nucleic acid amplification reactions such as polymerase chain reaction (PCR). Methods for preparing the modified detergents are also described.

公开(公告)号：US20230181742A1

公开(公告)日：2023-06-15

申请号：US17821122

申请日：2022-08-19

Applicant: Life Technologies Corporation

Inventor： Zhiwei Yang ,  Parul Angrish ,  Xavier de Mollerat Du Jeu ,  Kristin Wiederholt

IPC: A61K47/54 ,  C07C227/06 ,  C07D211/28 ,  C07D211/58 ,  C12N15/88 ,  A61K48/00 ,  C07C229/08 ,  C12N15/113

CPC classification number: A61K47/54 ,  C07C227/06 ,  C07D211/28 ,  C07D211/58 ,  C12N15/88 ,  A61K48/0008 ,  C07C229/08 ,  C12N15/1137 ,  A61K47/543 ,  C12N2310/11 ,  C12N2310/141

Abstract: There are provided for herein novel amine-containing transfection compounds and methods for making and using same. The compounds are generally obtained by reacting a primary amine with an unsaturated compound. Transfection complexes made using the amine-containing transfection compounds in combination with additional compounds to encapsulate biologically active agents such as nucleic acids are also provided for herein. Methods of using the transfection complexes for the in vivo or in vitro delivery of biologically active agents are also described. The transfection complexes of the present invention are highly potent, thereby allowing effective modulation of a biological activity at relatively low doses compared to analogous transfection compounds known in the art.

公开(公告)号：US11479814B2

公开(公告)日：2022-10-25

申请号：US16866211

申请日：2020-05-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Parul Angrish ,  Zhiwei Yang

IPC: C12Q1/68 ,  C12Q1/686 ,  C07D207/16 ,  C07C41/22 ,  C07C211/63 ,  C07C303/28 ,  C07D211/60 ,  C11D1/88 ,  C12N9/12 ,  C12N9/96

Abstract: This disclosure relates to novel compounds for use in various compositions, kits and methods, including, for example, use in polymerase storage buffers and in nucleic acid synthesis or amplification reactions such as a polymerase chain reaction (PCR). Methods for preparing the novel compounds are also described.

公开(公告)号：US10683539B2

公开(公告)日：2020-06-16

申请号：US15882789

申请日：2018-01-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Parul Angrish ,  Zhiwei Yang

IPC: C12Q1/68 ,  C12Q1/686 ,  C07D207/16 ,  C07C41/22 ,  C07C211/63 ,  C07C303/28 ,  C07D211/60 ,  C11D1/88 ,  C12N9/12 ,  C12N9/96

Abstract: This disclosure relates to novel compounds for use in various compositions, kits and methods, including, for example, use in polymerase storage buffers and in nucleic acid synthesis or amplification reactions such as a polymerase chain reaction (PCR). Methods for preparing the novel compounds are also described.

公开(公告)号：US10202639B2

公开(公告)日：2019-02-12

申请号：US15273824

申请日：2016-09-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Parul Angrish ,  Zhiwei Yang ,  Jonathan Wang

IPC: C12Q1/68 ,  C12Q1/6848 ,  C07C229/12 ,  C07C229/24 ,  C07D207/16 ,  C07D211/60 ,  C12Q1/6844 ,  C12Q1/6806 ,  C12Q1/6876

Abstract: This disclosure relates to novel detergents for use in various procedures including, for example, nucleic acid amplification reactions such as polymerase chain reaction (PCR). Methods for preparing the modified detergents are also described.

公开(公告)号：US20150174260A1

公开(公告)日：2015-06-25

申请号：US14340395

申请日：2014-07-24

Applicant: Life Technologies Corporation

Inventor： Zhiwei Yang ,  Parul Angrish ,  Xavier de Mollerat du Jeu ,  Kristin Wiederholt ,  Tao Wu

IPC: A61K47/48 ,  C12N15/113 ,  A61K48/00 ,  C07C229/08

CPC classification number: A61K47/54 ,  A61K47/543 ,  A61K48/0008 ,  C07C227/06 ,  C07C229/08 ,  C07D211/28 ,  C07D211/58 ,  C12N15/1137 ,  C12N15/88 ,  C12N2310/11 ,  C12N2310/141 ,  C07C229/26

Abstract: There are provided for herein novel amine-containing transfection compounds and methods for making and using same. The compounds are generally obtained by reacting a primary amine with an unsaturated compound. Transfection complexes made using the amine-containing transfection compounds in combination with additional compounds to encapsulate biologically active agents such as nucleic acids are also provided for herein. Methods of using the transfection complexes for the in vivo or in vitro delivery of biologically active agents are also described. The transfection complexes of the present invention are highly potent, thereby allowing effective modulation of a biological activity at relatively low doses compared to analogous transfection compounds known in the art.

Abstract translation: 本文提供了新颖的含胺转染化合物及其制备和使用方法。 化合物通常通过使伯胺与不饱和化合物反应而获得。 本文还提供了使用含胺转染化合物与附加化合物组合以包封生物活性剂如核酸制备的转染复合物。 还描述了使用转染复合物用于体内或体外递送生物活性剂的方法。 与本领域已知的类似转染化合物相比，本发明的转染复合物是非常有效的，从而允许相对低剂量的生物活性的有效调节。

公开(公告)号：US10676785B2

公开(公告)日：2020-06-09

申请号：US16225261

申请日：2018-12-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Parul Angrish ,  Zhiwei Yang ,  Jonathan Wang

IPC: C07C229/18 ,  C07C229/24 ,  C12Q1/6848 ,  C07C229/12 ,  C07D207/16 ,  C12Q1/6844 ,  C07D211/60 ,  C12Q1/6806 ,  C12Q1/6876

Abstract: This disclosure relates to novel detergents for use in various procedures including, for example, nucleic acid amplification reactions such as polymerase chain reaction (PCR). Methods for preparing the modified detergents are also described.

公开(公告)号：US10406237B2

公开(公告)日：2019-09-10

申请号：US15844380

申请日：2017-12-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Zhiwei Yang ,  Parul Angrish ,  Xavier de Mollerat du Jeu ,  Kristin Wiederholt

IPC: A61K47/54 ,  C07C227/06 ,  C07D211/28 ,  C07D211/58 ,  C12N15/88 ,  A61K48/00 ,  C07C229/08 ,  C12N15/113

Abstract: There are provided for herein novel amine-containing transfection compounds and methods for making and using same. The compounds are generally obtained by reacting a primary amine with an unsaturated compound. Transfection complexes made using the amine-containing transfection compounds in combination with additional compounds to encapsulate biologically active agents such as nucleic acids are also provided for herein. Methods of using the transfection complexes for the in vivo or in vitro delivery of biologically active agents are also described. The transfection complexes of the present invention are highly potent, thereby allowing effective modulation of a biological activity at relatively low doses compared to analogous transfection compounds known in the art.