公开(公告)号：US20230340554A1

公开(公告)日：2023-10-26

申请号：US18081582

申请日：2022-12-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Zhoutao CHEN ,  Xiaoping DUAN ,  Kyusung PARK

IPC: C12P19/40 ,  C12N15/10 ,  C12Q1/6806

CPC classification number: C12P19/40 ,  C12N15/1093 ,  C12Q1/6806 ,  C12N15/10

Abstract: In some embodiments, the present teachings provide compositions, systems, methods and kits for generating a population of nucleic acid fragments. In some embodiments, nucleic acids can be fragmented enzymatically. For example, methods for generating a population of nucleic acid fragments can include a nucleic acid nicking reaction. In one embodiment, the methods can include a nick translation reaction. A nicking reaction can introduce nicks at random positions on either strand of a double-stranded nucleic acid. A nick translation reaction can move the position of nicks to a new position so that the new positions of two of the nicks are aligned to create a double-stranded break. In some embodiments, methods for generating a population of nucleic acid fragments can include joining at least one end of a fragmented nucleic acid to one or more oligonucleotide adaptors.

公开(公告)号：US20140343265A1

公开(公告)日：2014-11-20

申请号：US14315140

申请日：2014-06-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Zhoutao CHEN ,  Xiaoping DUAN ,  Kyusung PARK

IPC: C12P19/40

CPC classification number: C12P19/40 ,  C12N15/10 ,  C12N15/1093 ,  C12Q1/6806 ,  C12Q2521/301 ,  C12Q2521/101 ,  C12Q2521/307 ,  C12Q2521/501 ,  C12Q2525/191

Abstract: In some embodiments, the present teachings provide compositions, systems, methods and kits for generating a population of nucleic acid fragments. In some embodiments, nucleic acids can be fragmented enzymatically. For example, methods for generating a population of nucleic acid fragments can include a nucleic acid nicking reaction. In one embodiment, the methods can include a nick translation reaction. A nicking reaction can introduce nicks at random positions on either strand of a double-stranded nucleic acid. A nick translation reaction can move the position of nicks to a new position so that the new positions of two of the nicks are aligned to create a double-stranded break. In some embodiments, methods for generating a population of nucleic acid fragments can include joining at least one end of a fragmented nucleic acid to one or more oligonucleotide adaptors.

Abstract translation: 在一些实施方案中，本教导提供了用于产生核酸片段群体的组合物，系统，方法和试剂盒。 在一些实施方案中，核酸可以酶促碎裂。 例如，产生核酸片段群的方法可以包括核酸切口反应。 在一个实施方案中，所述方法可以包括切口平移反应。 切口反应可以在双链核酸的任一链上的随机位置引入缺口。 切口平移反应可以将切口的位置移动到新位置，使得两个切口的新位置对齐以产生双链断裂。 在一些实施方案中，用于产生核酸片段群的方法可包括将片段化核酸的至少一端连接至一个或多个寡核苷酸衔接子。