公开(公告)号：US20240263247A1

公开(公告)日：2024-08-08

申请号：US18635799

申请日：2024-04-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David MERRILL ,  Pius BRZOSKA ,  Zheng LI ,  Wendy LIN ,  Wing LEE ,  Mandi WONG

IPC: C12Q1/6886 ,  C12Q1/6851 ,  C12Q1/6858

CPC classification number: C12Q1/6886 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/166

Abstract: Disclosed herein is a method including one or more of amplifying a nucleic acid sequence of interest in a sample comprising genomic DNA of a subject; amplifying a reference nucleic acid sequence in the sample; quantifying the amplified sequence of interest relative to the amplified reference sequence; and determining a copy number of the sequence of interest from the relative quantified amplified sequence of interest. The reference sequence can have at least 80% sequence identity to at least one of SEQ ID NO:1-38. Also disclosed are kits, systems and compositions that can include a first probe which specifically hybridizes to at least a portion of at least one reference sequence.

公开(公告)号：US20210254181A1

公开(公告)日：2021-08-19

申请号：US17301976

申请日：2021-04-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David MERRILL ,  Pius BRZOSKA ,  Zheng LI ,  Wendy LIN ,  Wing LEE ,  Mandi WONG

IPC: C12Q1/6886 ,  C12Q1/6858 ,  C12Q1/6851

Abstract: A method, comprising amplifying a nucleic acid sequence of interest in a sample comprising genomic DNA of a subject; amplifying a reference nucleic acid sequence in the sample; quantifying the amplified sequence of interest relative to the amplified reference sequence; and determining a copy number of the sequence of interest from the relative quantified amplified sequence of interest. The reference sequence may have at least 80% sequence identity to at least one of SEQ ID NO:1-38, such as SEQ ID NO:1-13. Also disclosed are kits and compositions, each comprising a first probe which specifically hybridizes to at least a portion of at least one reference sequence. Also disclosed is a system configured to perform the above method.

公开(公告)号：US20170096711A1

公开(公告)日：2017-04-06

申请号：US15128076

申请日：2015-03-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David MERRILL ,  Pius BRZOSKA ,  Zheng LI ,  Wendy LIN ,  Wing LEE ,  Mandi WONG

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/166 ,  C12Q2537/16 ,  C12Q2543/10 ,  C12Q2545/101 ,  C12Q2547/107 ,  C12Q2561/101

Abstract: A method, comprising amplifying a nucleic acid sequence of interest in a sample comprising genomic DNA of a subject; amplifying a reference nucleic acid sequence in the sample; quantifying the amplified sequence of interest relative to the amplified reference sequence; and determining a copy number of the sequence of interest from the relative quantified amplified sequence of interest. The reference sequence may have at least 80% sequence identity to at least one of SEQ ID NO:1-38, such as SEQ ID NO:1-13. Also disclosed are kits and compositions, each comprising a first probe which specifically hybridizes to at least a portion of at least one reference sequence. Also disclosed is a system configured to perform the above method.