公开(公告)号：US20220026365A1

公开(公告)日：2022-01-27

申请号：US17378479

申请日：2021-07-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark F. Oldham ,  Eric S. Nordman ,  Hongye Sun ,  Steven Boege

IPC: G01N21/64

Abstract: A scanning detection system is provided wherein emissions from locations in a flow cell are detected. In some embodiments, the system can comprise an excitation source, a photocleavage source, and modulating optics configured to cause an excitation beam generated by the excitation source to irradiate a first group of the fixed locations and to cause a photocleavage beam generated by the photocleavage source to irradiate a second group of the fixed locations, which is separate and apart from the first group of fixed locations. Methods of detecting sequencing reactions using such a system are also provided.

公开(公告)号：US20130292559A1

公开(公告)日：2013-11-07

申请号：US13853958

申请日：2013-03-29

Applicant: Life Technologies Corporation

Inventor： Stephen J. Gunstream ,  Mark F. Oldham

IPC: G01J1/58

CPC classification number: G01J1/58 ,  G01N21/278 ,  G01N21/6452 ,  Y10T436/10 ,  Y10T436/11 ,  Y10T436/112499 ,  Y10T436/113332

Abstract: Implementations of the present invention describe an apparatus for generating calibration factors for a spectral detector instrument. The calibration factors are derived from a calibration plate containing one or more spectral species in each well of the calibration plate. Each well is then exposed to an excitation source that causes the one or more spectral species in each of the wells to fluoresce. The signal response is measured and associated with each spectral species at each different well position in the calibration plate. Next, the measured signal response from each spectral species at each well position in the calibration plate is compared with a predetermined signal response for each spectral species. The results of this comparison can be used to determine a calibration factor for each well and spectral species to compensate for the difference between the measured signal response and the predetermined signal response.

Abstract translation: 本发明的实施方式描述了一种用于产生光谱检测器仪器的校准因子的装置。 校准因子来源于校准板的每个孔中含有一个或多个光谱种​​类的校准板。 然后将每个孔暴露于激发源，使得每个孔中的一个或多个光谱物质发荧光。 测量信号响应并与校准板中每个不同孔位置处的每个光谱种类相关联。 接下来，将来自校准板中每个孔位置处的每个光谱种类的测量信号响应与每个光谱种类的预定信号响应进行比较。 该比较的结果可用于确定每个孔和光谱种类的校准因子，以补偿测量的信号响应与预定信号响应之间的差异。

公开(公告)号：US11092548B2

公开(公告)日：2021-08-17

申请号：US16155831

申请日：2018-10-09

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark F. Oldham ,  Eric S. Nordman ,  Hongye Sun ,  Steven Boege

IPC: G01N21/00 ,  G01N21/64

Abstract: A scanning detection system is provided wherein emissions from locations in a flow cell are detected. In some embodiments, the system can comprise an excitation source, a photocleavage source, and modulating optics configured to cause an excitation beam generated by the excitation source to irradiate a first group of the fixed locations and to cause a photocleavage beam generated by the photocleavage source to irradiate a second group of the fixed locations, which is separate and apart from the first group of fixed locations. Methods of detecting sequencing reactions using such a system are also provided.

公开(公告)号：US20150218630A1

公开(公告)日：2015-08-06

申请号：US14557753

申请日：2014-12-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Hongye SUN ,  Mark F. Oldham ,  John O'Neill ,  Charles R. Connell ,  Umberto Ulmanella ,  Aldrich N.K. Lau ,  Theo Kotseroglou ,  Kenneth J. Livak

IPC: C12Q1/68 ,  G01N27/414

CPC classification number: C12Q1/6869 ,  B01L3/502761 ,  G01N27/3278 ,  G01N27/414 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/5438 ,  Y10S977/924 ,  Y10T436/143333 ,  C12Q2565/631

Abstract: In some embodiments, an analyte detection system is provided that includes a nanochannel, an electrode arrangement, and a plurality of nanoFET devices disposed in the nanochannel. A plurality of nucleic acid base detection components can be used that include a plurality of nanopores, a plurality of nanochannels, a plurality of hybridization probes, combinations thereof, and the like. According to other embodiments of the present teachings, different coded molecules are hybridized to a target DNA molecule and used to detect the presence of various sequences along the target molecule. A kit including mixtures of coded molecules is also provided. In some embodiments, devices including nanochannels, nanopores, and the like, are used for manipulating movement of DNA molecules, for example, in preparation for a DNA sequencing detection. Nanopore structures and methods of making the same are also provided as are methods of nucleic acid sequencing using the nanopore structures. Surface-modified nanopores are provided as are methods of making them. In some embodiments, surfaced-modified nanopores for slowing the translocation of single stranded DNA (ssDNA) through the nanopore are provided, as are nanopores configured to detect each of a plurality of different bases on an ssDNA strand.

Abstract translation: 在一些实施例中，提供了分析物检测系统，其包括纳米通道，电极布置以及设置在纳米通道中的多个纳米器件器件。 可以使用多个核酸碱基检测组分，其包括多个纳米孔，多个纳米通道，多个杂交探针及其组合等。 根据本教导的其他实施方案，将不同的编码分子与靶DNA分子杂交并用于检测沿着靶分子的各种序列的存在。 还提供了包含编码分子混合物的试剂盒。 在一些实施方案中，包括纳米通道，纳米孔等的装置用于操纵DNA分子的移动，例如用于DNA测序检测的准备。 还提供了制备其的纳米孔结构及其制备方法，使用纳米孔结构的核酸测序方法也是如此。 提供表面改性的纳米孔是制备它们的方法。 在一些实施方案中，提供了用于减缓通过纳米孔的单链DNA（ssDNA）易位的表面改性的纳米孔，以及配置成检测ssDNA链上的多个不同碱基中的每一个的纳米孔。

公开(公告)号：US10107758B2

公开(公告)日：2018-10-23

申请号：US14323203

申请日：2014-07-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark F. Oldham ,  Eric S. Nordman ,  Hongye Sun ,  Steven J. Boege

IPC: G01N21/64

Abstract: A scanning detection system is provided wherein emissions from locations in a flow cell are detected. In some embodiments, the system can comprise an excitation source, a photocleavage source, and modulating optics configured to cause an excitation beam generated by the excitation source to irradiate a first group of the fixed locations and to cause a photocleavage beam generated by the photocleavage source to irradiate a second group of the fixed locations, which is separate and apart from the first group of fixed locations. Methods of detecting sequencing reactions using such a system are also provided.

公开(公告)号：US09528152B2

公开(公告)日：2016-12-27

申请号：US14557753

申请日：2014-12-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Hongye Sun ,  Mark F. Oldham ,  John O'Neill ,  Charles R. Connell ,  Umberto Ulmanella ,  Aldrich N. K. Lau ,  Theo Kotseroglou ,  Kenneth J. Livak

IPC: C12Q1/68 ,  G01N27/414 ,  G01N33/543 ,  B01L3/00

CPC classification number: C12Q1/6869 ,  B01L3/502761 ,  G01N27/3278 ,  G01N27/414 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/5438 ,  Y10S977/924 ,  Y10T436/143333 ,  C12Q2565/631

Abstract: In some embodiments, an analyte detection system is provided that includes a nanochannel, an electrode arrangement, and a plurality of nanoFET devices disposed in the nanochannel. A plurality of nucleic acid base detection components can be used that include a plurality of nanopores, a plurality of nanochannels, a plurality of hybridization probes, combinations thereof, and the like. According to other embodiments of the present teachings, different coded molecules are hybridized to a target DNA molecule and used to detect the presence of various sequences along the target molecule. A kit including mixtures of coded molecules is also provided. In some embodiments, devices including nanochannels, nanopores, and the like, are used for manipulating movement of DNA molecules, for example, in preparation for a DNA sequencing detection. Nanopore structures and methods of making the same are also provided as are methods of nucleic acid sequencing using the nanopore structures. Surface-modified nanopores are provided as are methods of making them. In some embodiments, surfaced-modified nanopores for slowing the translocation of single stranded DNA (ssDNA) through the nanopore are provided, as are nanopores configured to detect each of a plurality of different bases on an ssDNA strand.