公开(公告)号：US11872285B2

公开(公告)日：2024-01-16

申请号：US17063677

申请日：2020-10-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Xavier de Mollerat du Jeu ,  Nektaria Andronikou

IPC: A61K47/18 ,  A61K9/127 ,  A61K31/7105 ,  C12N15/88

CPC classification number: A61K47/186 ,  A61K9/1272 ,  A61K31/7105 ,  C12N15/88

Abstract: The present invention provides lipid aggregate compositions having at least two cationic lipids in combination with one or optionally more than one non-cationic lipids for the in vitro and/or in vivo delivery of biologically active molecules including nucleic acids, specifically DNA and RNA, and proteins into cells and tissues. Methods are also provided that use the compounds of the present invention to deliver biologically active molecules, into cells and tissues to facilitate the expression of target proteins therein. In some non-limiting embodiments, the subject lipid aggregate compositions can be used to deliver nucleic acid molecules to facilitate the expression of proteins involved in cellular reprogramming and genome editing applications.

公开(公告)号：US10792362B2

公开(公告)日：2020-10-06

申请号：US16230393

申请日：2018-12-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Xavier de Mollerat du Jeu ,  Nektaria Andronikou

IPC: A61K47/18 ,  A61K9/127 ,  A61K31/7105 ,  C12N15/88

Abstract: The present invention provides lipid aggregate compositions having at least two cationic lipids in combination with one or optionally more than one non-cationic lipids for the in vitro and/or in vivo delivery of biologically active molecules including nucleic acids, specifically DNA and RNA, and proteins into cells and tissues. Methods are also provided that use the compounds of the present invention to deliver biologically active molecules, into cells and tissues to facilitate the expression of target proteins therein. In some non-limiting embodiments, the subject lipid aggregate compositions can be used to deliver nucleic acid molecules to facilitate the expression of proteins involved in cellular reprogramming and genome editing applications.

公开(公告)号：US20240263124A1

公开(公告)日：2024-08-08

申请号：US18624847

申请日：2024-04-02

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES HOLDINGS PTE LTD

Inventor： Steven Huei Yeo ,  Xavier De Mollerat Du Jeu ,  Way Xuang Lee ,  Nektaria Andronikou ,  Benyong Shi ,  Kok Shyong Chong ,  Han Wei ,  Siu Wee Hon ,  Yvonne Peck ,  Chee Wai Chan ,  Beng Heng Lim ,  Stacey Mei Lin Teo ,  Dhanaraj Shanmugam ,  Jun Yan Tham ,  Michael Gordon ,  Chris Rossman ,  Ulrich Forke ,  Li Yang Lim

IPC: C12M1/42 ,  C12M1/00 ,  C12M1/06 ,  C12M1/34 ,  C12M1/36 ,  C12M3/00

CPC classification number: C12M35/02 ,  C12M23/14 ,  C12M23/44 ,  C12M27/02 ,  C12M29/00 ,  C12M41/12 ,  C12M41/48

Abstract: The present disclosure provides electroporation cartridges for single use electroporation as well as electroporation cartridges for automated batch processing, electroporation instruments and systems and methods of electroporation using these devices and systems. In some embodiments, electroporation cartridges comprise an electroporation chamber defined by an elongate body, a first electrode at a proximal end and a second electrode at a distal end of a chamber. Electroporation systems of the disclosure include one or more components including a pulse generator, compartments for placing either flow-through or single use electroporation cartridges, components for storage of cells, cooling and pre-cooling mechanisms, removably insertable modular casings having compartments for holding and arranging electroporation system and reagent components, one or more pumps for moving sample through the system, and processors and controllers.

公开(公告)号：US20230110090A1

公开(公告)日：2023-04-13

申请号：US17960237

申请日：2022-10-05

Applicant: Life Technologies Holdings PTE Limited ,  Life Technologies Corporation

Inventor： Mio Xiu Lu Ling ,  Harmon Cosme Sicat, JR. ,  Joshua Mead ,  Benyong Shi ,  Li Yong Ong ,  Don Paul Kovarcik ,  Nektaria Andronikou

IPC: C12M1/42 ,  C12M1/36

Abstract: The present disclosure provides systems and methods of electroporation protocol optimization. Embodiments include electroporation machines capable of carrying out test protocols including multiple user-designated parameters. The protocols and parameters can be carried out on samples comprising cells, including portions of a sample, to determine optimum parameters for electroporation for different samples. The systems and methods of optimization preferably use electroporation cartridges, electroporation instruments and systems and methods of electroporation using these devices and systems. In some embodiments, electroporation cartridges comprise an electroporation chamber and electrodes.

公开(公告)号：US20240400964A1

公开(公告)日：2024-12-05

申请号：US18689963

申请日：2022-10-06

Applicant: Life Technologies Holdings PTE Ltd ,  Life Technologies Corporation

Inventor： Mio Xiu Lu Ling ,  Nektaria Andronikou ,  Joshua Mead ,  Benyong Shi ,  Don Paul Kovarcik ,  Harmon Jr Cosme Sicat ,  Li Yong Ong

IPC: C12M1/42 ,  C12M1/36

Abstract: The present disclosure provides systems and methods of electroporation protocol optimization. Embodiments include electroporation machines capable of carrying out test protocols including multiple user-designated parameters. The protocols and parameters can be carried out on samples comprising cells, including portions of a sample, to determine optimum parameters for electroporation for different samples. The systems and methods of optimization preferably use electroporation cartridges, electroporation instruments and systems and methods of electroporation using these devices and systems. In some embodiments, electroporation cartridges comprise an electroporation chamber and electrodes.

公开(公告)号：US10195280B2

公开(公告)日：2019-02-05

申请号：US14800565

申请日：2015-07-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Xavier de Mollerat du Jeu ,  Nektaria Andronikou

IPC: A61K47/18 ,  A61K9/127 ,  A61K31/7105 ,  C12N15/88

Abstract: The present invention provides lipid aggregate compositions having at least two cationic lipids in combination with one or optionally more than one non-cationic lipids for the in vitro and/or in vivo delivery of biologically active molecules including nucleic acids, specifically DNA and RNA, and proteins into cells and tissues. Methods are also provided that use the compounds of the present invention to deliver biologically active molecules, into cells and tissues to facilitate the expression of target proteins therein. In some non-limiting embodiments, the subject lipid aggregate compositions can be used to deliver nucleic acid molecules to facilitate the expression of proteins involved in cellular reprogramming and genome editing applications.