公开(公告)号：US20160108079A1

公开(公告)日：2016-04-21

申请号：US14952149

申请日：2015-11-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Zhaochun MA ,  Khairuzzaman Bashar MULLAH ,  Robert G. EASON

IPC: C07H19/16

CPC classification number: C07H19/16 ,  C07D239/54 ,  C07D405/04 ,  C07D473/18 ,  C07D473/34 ,  C07H19/06 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/6806

Abstract: Methods for preparation of 2′,3′-dideoxynucleotides support structures, such as 2′,3′-dideoxyguanosine, 2′,3′-dideoxyadenosine, and 3′-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated DNA synthesis and pyrophosphorolysis activated polymerization.

Abstract translation: 公开了制备2'，3'-二脱氧核苷酸支持结构的方法，例如2'，3'-二脱氧鸟苷，2'，3'-二脱氧腺苷和3'-脱氧胸苷支持结构。 还提供了使用这些结构的各种方法，例如其用于自动DNA合成和焦磷酸裂解活化聚合的用途。

公开(公告)号：US20150337300A1

公开(公告)日：2015-11-26

申请号：US14816920

申请日：2015-08-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Vladimir I. BASHKIROV ,  Umberto ULMANELLA ,  Robert G. EASON ,  Bradford J. TAFT

IPC: C12N15/10

CPC classification number: C12Q1/6806 ,  C07H1/06 ,  C07H21/00 ,  C12N15/1093 ,  C12Q2523/301

Abstract: Methods and kits for preparing nucleic acid fragments from a sample of purified nucleic acid are provided. Alternatively, chromatin or other long polymers can be sheared with similar methods and kits.