公开(公告)号：US20190211373A1

公开(公告)日：2019-07-11

申请号：US16247267

申请日：2019-01-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Linda Lee ,  Sam Woo ,  Peter Ma

IPC: C12Q1/686 ,  C12Q1/6874 ,  C12Q1/6869

CPC classification number: C12Q1/686 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2525/113 ,  C12Q2525/125

Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.

公开(公告)号：US11104944B2

公开(公告)日：2021-08-31

申请号：US16247267

申请日：2019-01-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Linda Lee ,  Sam Woo ,  Peter Ma

IPC: C12Q1/68 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q1/6853 ,  C12Q1/6869

Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.

公开(公告)号：US12006536B2

公开(公告)日：2024-06-11

申请号：US17399449

申请日：2021-08-11

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Linda Lee ,  Sam Woo ,  Peter Ma

IPC: C12Q1/68 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874

CPC classification number: C12Q1/686 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2525/113 ,  C12Q2525/125 ,  C12Q1/6874 ,  C12Q2525/113 ,  C12Q2525/125

Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.

公开(公告)号：US10221449B2

公开(公告)日：2019-03-05

申请号：US15652224

申请日：2017-07-17

Applicant: Life Technologies Corporation

Inventor： Linda Lee ,  Sam Woo ,  Peter Nien-Tung Ma

IPC: C12Q1/68 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q1/6869

Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.

公开(公告)号：US09708650B2

公开(公告)日：2017-07-18

申请号：US14198308

申请日：2014-03-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Linda Lee ,  Sam Woo ,  Peter Ma

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2525/113 ,  C12Q2525/125

Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.

公开(公告)号：US09410192B2

公开(公告)日：2016-08-09

申请号：US14053571

申请日：2013-10-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Linda Lee ,  Sam Woo ,  Peter Ma

IPC: C12P19/34 ,  C12Q1/68 ,  C07H21/00

CPC classification number: C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2525/113 ,  C12Q2525/125

Abstract: A composition is provided comprising a negatively charged group, an oligonucleotide sequence and at least none or one nuclease-resistant linkage group to form a chemically-enhanced primer. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the primer as well as a method of preparing DNA for sequencing and a method of sequencing DNA and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition under conditions in which excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.

Abstract translation: 提供了一种组合物，其包含带负电荷的基团，寡核苷酸序列和至少一个或一个核酸酶抗性连锁基团以形成化学增强的引物。 化学增强引物可用于测序和片段分析。 还提供了合成引物的方法以及用于测序的DNA的制备方法和测序DNA的方法以及含有化学增强的引物的试剂盒。 DNA测序的方法可以包括使扩增反应产物与组合物在过量扩增引物被核酸酶降解的条件下接触，化学增强引物基本上不降解。

公开(公告)号：US20140377747A1

公开(公告)日：2014-12-25

申请号：US14198308

申请日：2014-03-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Linda LEE ,  Sam Woo ,  Peter Ma

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2525/113 ,  C12Q2525/125

Abstract: A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.

Abstract translation: 提供化学增强的引物，其包含带负电荷的部分（NCM），具有a）非核酸酶抗性核苷酸间连接的寡核苷酸序列或b）至少一个核酸酶抗性核苷酸间连接。 化学增强引物可用于测序和片段分析。 还提供了用于合成化学增强引物的方法以及制备用于测序的DNA的方法，测序DNA的方法和含有化学增强的引物的试剂盒。 测序DNA的方法可以包括使扩增反应产物与其中过量扩增引物被核酸酶降解并且化学增强引物基本上未降解的组合物接触。