公开(公告)号：US20160025676A1

公开(公告)日：2016-01-28

申请号：US14728471

申请日：2015-06-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Thomas Beardslee ,  Timothy Updyke

IPC: G01N27/447

CPC classification number: G01N27/44756 ,  C07K1/26 ,  C08J3/075 ,  C08J2333/26 ,  C08K5/0008 ,  C08L33/26 ,  G01N27/44747

Abstract: Gels, such as polyacrylamide gels, are provided that include linear polyacrylamide in the stacking gel. Native gels that include linear polyacrylamide in the stacker can be used to separate biomolecular complexes, such as protein complexes. Gel cassettes in which the gap width between front and back plates does not vary by more than 5% at the upper edge of the cassette are also provided. The gel cassettes can be used for electrophoretic separation of proteins and protein complexes on native gels, such as native gels that include linear polyacrylamide in the stacker. The native gels can have multiple wells for electrophoresing at least one sample and/or at least one molecular weight standard.

Abstract translation: 提供凝胶，如聚丙烯酰胺凝胶，其包括在聚集凝胶中的线性聚丙烯酰胺。 在堆垛机中包括线性聚丙烯酰胺的天然凝胶可用于分离生物分子复合物，例如蛋白质复合物。 还提供了其中前板和后板之间的间隙宽度在盒的上边缘处不变化大于5％的凝胶盒。 凝胶盒可以用于天然凝胶上的蛋白质和蛋白质复合物的电泳分离，例如在堆积器中包括线性聚丙烯酰胺的天然凝胶。 天然凝胶可以具有多个用于电泳至少一个样品和/或至少一个分子量标准的孔。

公开(公告)号：US09057694B2

公开(公告)日：2015-06-16

申请号：US13928137

申请日：2013-06-26

Applicant: Life Technologies Corporation

Inventor： Timothy Updyke ,  Thomas Beardslee

IPC: G01N27/447 ,  C07K1/26 ,  C08J3/075 ,  C08K5/00 ,  C08L33/26

CPC classification number: G01N27/44756 ,  C07K1/26 ,  C08J3/075 ,  C08J2333/26 ,  C08K5/0008 ,  C08L33/26 ,  G01N27/44747

Abstract: Gels, such as polyacrylamide gels, are provided that include linear polyacrylamide in the stacking gel. Native gels that include linear polyacrylamide in the stacker can be used to separate biomolecular complexes, such as protein complexes. Gel cassettes in which the gap width between front and back plates does not vary by more than 5% at the upper edge of the cassette are also provided. The gel cassettes can be used for electrophoretic separation of proteins and protein complexes on native gels, such as native gels that include linear polyacrylamide in the stacker. The native gels can have multiple wells for electrophoresing at least one sample and/or at least one molecular weight standard.

Abstract translation: 提供凝胶，如聚丙烯酰胺凝胶，其包括在聚集凝胶中的线性聚丙烯酰胺。 在堆垛机中包括线性聚丙烯酰胺的天然凝胶可用于分离生物分子复合物，例如蛋白质复合物。 还提供了其中前板和后板之间的间隙宽度在盒的上边缘处不变化大于5％的凝胶盒。 凝胶盒可以用于天然凝胶上的蛋白质和蛋白质复合物的电泳分离，例如在堆积器中包括线性聚丙烯酰胺的天然凝胶。 天然凝胶可以具有多个用于电泳至少一个样品和/或至少一个分子量标准的孔。