公开(公告)号：US20210310056A1

公开(公告)日：2021-10-07

申请号：US17351409

申请日：2021-06-18

Applicant: LUMINEX CORPORATION

Inventor： Doug WHITMAN ,  Brian SCHRADER

IPC: C12Q1/6818 ,  C12Q1/6834

Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In one embodiment, a sample is contacted with a primer and a quencher-probe complementary to a target nucleic acid. The quencher-probe is complementary to an anti-probe that comprises a reporter and is attached to a solid support. Thus, hybridized probe is cleaved with a nucleic acid polymerase having exonuclease activity to release the quencher from the probe. The presence of the target nucleic acid is then detected and/or optionally quantified by detecting an increase in signal from the fluorescent reporter on the solid support.

公开(公告)号：US20160046995A1

公开(公告)日：2016-02-18

申请号：US14826518

申请日：2015-08-14

Applicant: LUMINEX CORPORATION

Inventor： Martin KOCHANCZYK ,  Doug WHITMAN

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q2521/327 ,  C12Q2525/121 ,  C12Q2525/131 ,  C12Q2525/301

Abstract: Nucleic acid constructs and methods that provide superior prevention of primer-dimers and other artifacts of false priming events are disclosed. In particular, there is disclosed a hairpin primer having a target-specific primer region, wherein the target-specific region comprises a target-binding dependent cleavage sequence; a first stem forming region 5′ of the target-specific primer region; and a second stem forming region 3′ of the target-specific primer region, wherein the second stem forming region is complementary to the first stem forming region. Methods of using the hairpin primer to amplify a target nucleic acid are also disclosed.

Abstract translation: 公开了提供优异的预防引物二聚体和假引发事件的其它伪像的核酸构建体和方法。 特别地，公开了具有靶特异性引物区的发夹引物，其中靶特异性区包含靶依赖性依赖性切割序列; 靶特异性引物区的第一茎形成区5'; 和靶特异性引物区的第二茎形成区3'，其中第二茎形成区与第一茎形成区互补。 还公开了使用发夹引物扩增靶核酸的方法。

公开(公告)号：US20160040219A1

公开(公告)日：2016-02-11

申请号：US14823288

申请日：2015-08-11

Applicant: LUMINEX CORPORATION

Inventor： Scott C. JOHNSON ,  Nicolas ARAB ,  Doug WHITMAN

IPC: C12Q1/68

CPC classification number: C12Q1/6818 ,  C12Q1/6816 ,  C12Q1/6823 ,  C12Q2521/301 ,  C12Q2521/327 ,  C12Q2525/101 ,  C12Q2525/121 ,  C12Q2525/161 ,  C12Q2525/186 ,  C12Q2525/301 ,  C12Q2527/107 ,  C12Q2537/143 ,  C12Q2561/113 ,  C12Q2565/101

Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes that comprise a ribonucleotide position that is susceptible to endoribonuclease (e.g., RNase H) cleavage in the presence of target nucleic acid molecules. Probes of the embodiments may also comprise non-natural nucleotide linked to a reporter and/or quenching moiety.

Abstract translation: 提供了用于检测和定量核酸的方法和组合物。 在某些实施方案中，方法涉及使用可切割探针，其可包含在靶核酸分子存在下内切核糖核酸酶（例如RNase H）裂解的核糖核苷酸位置。 实施方案的探针还可以包含与报道分子和/或猝灭部分连接的非天然核苷酸。

公开(公告)号：US20150044680A1

公开(公告)日：2015-02-12

申请号：US14456239

申请日：2014-08-11

Applicant: LUMINEX CORPORATION

Inventor： Doug WHITMAN ,  Nicolas Arab ,  Chuck Collins

IPC: C12Q1/68

CPC classification number: C12Q1/6818 ,  C12Q2525/186 ,  C12Q2527/107 ,  C12Q2561/109 ,  C12Q2525/161 ,  C12Q2525/301 ,  C12Q2533/101 ,  C12Q2537/143

Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of primers or probes that comprise a non-natural nucleotide linked to a reporter. Target nucleic acids are detected by the polymerization of a complementary probe or primer that incorporated a cognate non-natural nucleotide linked to a quencher.

Abstract translation: 提供了用于检测和定量核酸的方法和组合物。 在某些实施方案中，方法涉及使用包含与报告基团连接的非天然核苷酸的引物或探针。 通过掺入与猝灭剂连接的同源非天然核苷酸的互补探针或引物的聚合来检测靶核酸。

公开(公告)号：US20140378327A1

公开(公告)日：2014-12-25

申请号：US14307984

申请日：2014-06-18

Applicant: LUMINEX CORPORATION

Inventor： Doug WHITMAN ,  Brian Schrader

IPC: C12Q1/68

CPC classification number: C12Q1/6818 ,  C12Q1/6834 ,  C12Q2521/101 ,  C12Q2521/325 ,  C12Q2563/149

Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In one embodiment, a sample is contacted with a primer and a quencher-probe complementary to a target nucleic acid. The quencher-probe is complementary to an anti-probe that comprises a reporter and is attached to a solid support. Thus, hybridized probe is cleaved with a nucleic acid polymerase having exonuclease activity to release the quencher from the probe. The presence of the target nucleic acid is then detected and/or optionally quantified by detecting an increase in signal from the fluorescent reporter on the solid support.

Abstract translation: 提供了用于检测和定量核酸的方法和组合物。 在一个实施方案中，将样品与引物和与靶核酸互补的猝灭剂 - 探针接触。 猝灭剂探针与包含报道分子并附着于固体支持物的抗探针互补。 因此，杂交探针用具有核酸外切酶活性的核酸聚合酶切割以从探针释放猝灭剂。 然后通过检测固体支持物上来自荧光报告基团的信号的增加来检测和/或任选定量目标核酸的存在。

公开(公告)号：US20220282307A1

公开(公告)日：2022-09-08

申请号：US17824215

申请日：2022-05-25

Applicant: LUMINEX CORPORATION

Inventor： Doug WHITMAN

IPC: C12Q1/6818 ,  C12Q1/6876

Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes capable of forming double-stranded structures, such as hairpin structures, which probes can be distinguished from one another on the basis of reporter signal, melt properties, or both.

公开(公告)号：US20210189469A1

公开(公告)日：2021-06-24

申请号：US17196200

申请日：2021-03-09

Applicant: LUMINEX CORPORATION

Inventor： Scott C. JOHNSON ,  Nicolas ARAB ,  Doug WHITMAN

IPC: C12Q1/6818 ,  C12Q1/6816 ,  C12Q1/6823

Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes that comprise a ribonucleotide position that is susceptible to endoribonuclease (e.g., RNase H) cleavage in the presence of target nucleic acid molecules. Probes of the embodiments may also comprise non-natural nucleotide linked to a reporter and/or quenching moiety.

公开(公告)号：US20200263240A1

公开(公告)日：2020-08-20

申请号：US16869758

申请日：2020-05-08

Applicant: LUMINEX CORPORATION

Inventor： Martin KOCHANCZYK ,  Doug WHITMAN

IPC: C12Q1/6827

Abstract: Nucleic acid constructs and methods that provide superior prevention of primer-dimers and other artifacts of false priming events are disclosed. In particular, there is disclosed a hairpin primer having a target-specific primer region, wherein the target-specific region comprises a target-binding dependent cleavage sequence; a first stem forming region 5′ of the target-specific primer region; and a second stem forming region 3′ of the target-specific primer region, wherein the second stem forming region is complementary to the first stem forming region. Methods of using the hairpin primer to amplify a target nucleic acid are also disclosed.

公开(公告)号：US20170321259A1

公开(公告)日：2017-11-09

申请号：US15656541

申请日：2017-07-21

Applicant: LUMINEX CORPORATION

Inventor： Scott C. JOHNSON ,  Nicolas ARAB ,  Doug WHITMAN

IPC: C12Q1/68

Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes that comprise a ribonucleotide position that is susceptible to endoribonuclease (e.g., RNase H) cleavage in the presence of target nucleic acid molecules. Probes of the embodiments may also comprise non-natural nucleotide linked to a reporter and/or quenching moiety.

公开(公告)号：US20190093061A1

公开(公告)日：2019-03-28

申请号：US16202315

申请日：2018-11-28

Applicant: LUMINEX CORPORATION

Inventor： Doug WHITMAN ,  James HEISLER ,  Colin BOZARTH ,  Germaine SIEW ,  Charles COLLINS ,  Eric SMITH

IPC: C12M1/06 ,  B01F13/08 ,  B01L7/00

Abstract: Methods and systems for magnetic mixing. Particular embodiments relate to applying a magnetic field to move a magnetically responsive component in a chamber.