公开(公告)号：US09982291B2

公开(公告)日：2018-05-29

申请号：US14823288

申请日：2015-08-11

Applicant: LUMINEX CORPORATION

Inventor： Scott C. Johnson ,  Nicolas Arab ,  Doug Whitman

IPC: C12Q1/68 ,  C07H21/00 ,  C12P19/34

CPC classification number: C12Q1/6818 ,  C12Q1/6816 ,  C12Q1/6823 ,  C12Q2521/301 ,  C12Q2521/327 ,  C12Q2525/101 ,  C12Q2525/121 ,  C12Q2525/161 ,  C12Q2525/186 ,  C12Q2525/301 ,  C12Q2527/107 ,  C12Q2537/143 ,  C12Q2561/113 ,  C12Q2565/101

Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes that comprise a ribonucleotide position that is susceptible to endoribonuclease (e.g., RNase H) cleavage in the presence of target nucleic acid molecules. Probes of the embodiments may also comprise non-natural nucleotide linked to a reporter and/or quenching moiety.

公开(公告)号：US10975419B2

公开(公告)日：2021-04-13

申请号：US15962077

申请日：2018-04-25

Applicant: LUMINEX CORPORATION

Inventor： Scott C. Johnson ,  Nicolas Arab ,  Doug Whitman

IPC: C12Q1/6818 ,  C07H21/00 ,  C12Q1/6816 ,  C12Q1/6823

Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes that comprise a ribonucleotide position that is susceptible to endoribonuclease (e.g., RNase H) cleavage in the presence of target nucleic acid molecules. Probes of the embodiments may also comprise non-natural nucleotide linked to a reporter and/or quenching moiety.

公开(公告)号：US10752939B2

公开(公告)日：2020-08-25

申请号：US15656541

申请日：2017-07-21

Applicant: LUMINEX CORPORATION

Inventor： Scott C. Johnson ,  Nicolas Arab ,  Doug Whitman

IPC: C12Q1/68 ,  C07H21/00 ,  C12Q1/6818 ,  C12Q1/6816 ,  C12Q1/6823

Abstract: Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes that comprise a ribonucleotide position that is susceptible to endoribonuclease (e.g., RNase H) cleavage in the presence of target nucleic acid molecules. Probes of the embodiments may also comprise non-natural nucleotide linked to a reporter and/or quenching moiety.