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10 条记录 (耗时 0.031 秒)

    Formation of and methods for the production of large Bacillus thuringiensis crystals with increased pesticidal activity
    2.
    发明授权
    Formation of and methods for the production of large Bacillus thuringiensis crystals with increased pesticidal activity 失效
    生产具有增加的杀虫活性的大型苏云金芽孢杆菌晶体的形成和方法

    公开(公告)号：US06280720B1

    公开(公告)日：2001-08-28

    申请号：US08274608

    申请日：1994-07-13

    申请人： Lee Fremont Adams ,  Michael David Thomas ,  Alan P. Sloma ,  William R. Widner

    发明人： Lee Fremont Adams ,  Michael David Thomas ,  Alan P. Sloma ,  William R. Widner

    IPC分类号： A01N6300

    CPC分类号： A01N63/00 ,  C07K14/325 ,  C12N15/75

    摘要： The invention relates to a method for producing an integrant(s) of Bacillus thuringiensis which produces a larger quantity of a crystal delta-endotoxin greater pesticidal activity as compared to the crystal delta-endotoxin produced by the corresponding parental strain. The crystal delta-endotoxin produced by the integrant Bacillus thuringiensis will have an activity directed towards the same pest(s) as its parent Bacillus thuringiensis crystal delta-endotoxin. The invention further relates to such integrants, compositions comprising such integrants, as well as methods for controlling a pest(s) using these compositions.

    摘要翻译： 本发明涉及一种生产苏云金芽孢杆菌整合体的方法，与由相应的亲本菌株产生的晶体δ-内毒素相比，其产生更大量的结晶δ-内毒素，更大的杀虫活性。 由苏云金芽孢杆菌整合产生的晶体δ-内毒素将具有与其母体苏云金芽孢杆菌晶体δ-内毒素相同的害虫的活性。 本发明还涉及这样的整合剂，包含这种整合剂的组合物以及使用这些组合物来控制害虫的方法。

    Formation of and methods for the production of large bacillus thuringiensis crystals with increased pesticidal activity
    3.
    发明授权
    Formation of and methods for the production of large bacillus thuringiensis crystals with increased pesticidal activity 失效
    具有增加杀虫活性的大型苏云金芽孢杆菌晶体的生产和方法

    公开(公告)号：US06303382B1

    公开(公告)日：2001-10-16

    申请号：US08771190

    申请日：1996-12-20

    申请人： Lee Fremont Adams ,  Michael David Thomas ,  Alan P. Sloma ,  William R. Widner

    发明人： Lee Fremont Adams ,  Michael David Thomas ,  Alan P. Sloma ,  William R. Widner

    IPC分类号： C12N1563

    CPC分类号： A01N63/00 ,  C07K14/325 ,  C12N15/75

    摘要： The invention relates to a method for producing an integrant(s) of Bacillus thuringiensis which produces a larger quantity of a crystal delta-endotoxin with greater pesticidal activity as compared to the crystal delta-endotoxin produced by the corresponding parental strain. The crystal delta-endotoxin produced by the integrant Bacillus thuringiensis will have an activity directed towards the same pest(s) as its parent Bacillus thuringiensis crystal delta-endotoxin. The invention further relates to such integrants, compositions comprising such integrants, as well as methods for controlling a pest(s) using these compositions.

    摘要翻译： 本发明涉及一种生产苏云金芽孢杆菌综合体的方法，与由相应的亲本菌株产生的晶体δ-内毒素相比，产生更大量的具有更大农药活性的结晶δ-内毒素。 由苏云金芽孢杆菌整合产生的晶体δ-内毒素将具有与其母体苏云金芽孢杆菌晶体δ-内毒素相同的害虫的活性。 本发明还涉及这样的整合剂，包含这种整合剂的组合物以及使用这些组合物来控制害虫的方法。

    Bacterial donor cell useful in conjugation
    6.
    发明授权
    Bacterial donor cell useful in conjugation 失效
    用于共轭的细菌供体细胞

    公开(公告)号：US5763187A

    公开(公告)日：1998-06-09

    申请号：US785448

    申请日：1997-01-17

    申请人： Alan P. Sloma ,  William R. Widner

    发明人： Alan P. Sloma ,  William R. Widner

    IPC分类号： C12N15/75 ,  C12Q1/68 ,  C07H21/04 ,  C12N15/65 ,  C12P21/00

    CPC分类号： C12N15/75

    摘要： The present invention relates to a modified bacterial donor cell useful in conjugation, the cell containing i) a plasmid comprising a DNA construct encoding the polypeptide of interest, and at least one cis-acting DNA sequence required for the transfer of said plasmid by conjugation in the presence of a trans-acting mobilizing element, and ii) at least one DNA sequence encoding said trans-acting mobilizing element, wherein the donor cell has a reduced capacity, relative to a parent cell, of producing a bactericidal agent which kills or prevents growth in a population of recipient cells.

    摘要翻译： 本发明涉及可用于缀合的修饰的细菌供体细胞，所述细胞含有i）包含编码目的多肽的DNA构建体的质粒和通过共轭转移所述质粒所需的至少一种顺式作用DNA序列 反式作用动员元件的存在，以及ii）至少一个编码所述反式作用动员元件的DNA序列，其中所述供体细胞相对于亲本细胞具有降低的能力，其产生杀死或预防的杀菌剂 受体细胞群体的增长。

    Nucleic acid constructs for the production of a Bacillus alkaline protease
    9.
    发明授权
    Nucleic acid constructs for the production of a Bacillus alkaline protease 失效
    用于生产芽孢杆菌碱性蛋白酶的核酸构建体

    公开(公告)号：US5621089A

    公开(公告)日：1997-04-15

    申请号：US434255

    申请日：1995-05-03

    申请人： Alan P. Sloma ,  Helle Outtrup ,  Claus Dambmann ,  Dorrit A. Aaslyng

    发明人： Alan P. Sloma ,  Helle Outtrup ,  Claus Dambmann ,  Dorrit A. Aaslyng

    IPC分类号： C12N15/09 ,  C11D3/386 ,  C12N1/21 ,  C12N9/54 ,  C12N15/57 ,  C12N15/75 ,  C12N15/63 ,  C12N15/74

    CPC分类号： C11D3/386 ,  C12N15/75 ,  C12N9/54

    摘要： The invention is directed to an isolated nucleic acid construct(s) comprising a nucleic acid sequence encoding an alkaline protease having a molecular weight of about 34 kD and is obtainable from a strain of Bacillus sp. Group I, as well as recombinant vectors and host cells comprising such constructs. Additionally, the invention is directed to a method for obtaining the protease. The invention is also directed to promoter and signal sequences derived from said alkaline protease as well as methods of using the promoter and signal sequences in the expression of heterologous nucleic acid sequences encoding heterologous proteins in bacteria.

    摘要翻译： 本发明涉及分离的核酸构建体，其包含编码分子量为约34kD的碱性蛋白酶的核酸序列，并且可从芽孢杆菌属菌株获得。 组I，以及包含这种构建体的重组载体和宿主细胞。 另外，本发明涉及获得蛋白酶的方法。 本发明还涉及来自所述碱性蛋白酶的启动子和信号序列以及在编码细菌中的异源蛋白质的异源核酸序列的表达中使用启动子和信号序列的方法。

    Recombinant methods for the production of a bacillus alkaline protease
    10.
    发明授权
    Recombinant methods for the production of a bacillus alkaline protease 失效
    用于生产杆菌碱性蛋白酶的重组方法

    公开(公告)号：US5622850A

    公开(公告)日：1997-04-22

    申请号：US460327

    申请日：1995-06-02

    申请人： Alan P. Sloma ,  Helle Outtrup ,  Claus Dambmann ,  Dorrit A. Aaslyng

    发明人： Alan P. Sloma ,  Helle Outtrup ,  Claus Dambmann ,  Dorrit A. Aaslyng

    IPC分类号： C12N15/09 ,  C11D3/386 ,  C12N1/21 ,  C12N9/54 ,  C12N15/57 ,  C12N15/75 ,  C12N15/74

    CPC分类号： C11D3/386 ,  C12N15/75 ,  C12N9/54

    摘要： The invention is directed to an isolated nucleic acid construct(s) comprising a nucleic acid sequence encoding an alkaline protease having a molecular weight of about 34 kD and is obtainable from a strain of Bacillus sp. Group I, as well as recombinant vectors and host cells comprising such constructs. Additionally, the invention is directed to a method for obtaining the protease. The invention is also directed to promoter and signal sequences derived from said alkaline protease as well as methods of using the promoter and signal sequences in the expression of heterologous nucleic acid sequences encoding heterologous proteins in bacteria.

    摘要翻译： 本发明涉及分离的核酸构建体，其包含编码分子量为约34kD的碱性蛋白酶的核酸序列，并且可从芽孢杆菌属菌株获得。 组I，以及包含这种构建体的重组载体和宿主细胞。 另外，本发明涉及获得蛋白酶的方法。 本发明还涉及来自所述碱性蛋白酶的启动子和信号序列以及在编码细菌中的异源蛋白质的异源核酸序列的表达中使用启动子和信号序列的方法。