Nucleic acid constructs for the production of a Bacillus alkaline
protease
    2.
    发明授权
    Nucleic acid constructs for the production of a Bacillus alkaline protease 失效
    用于生产芽孢杆菌碱性蛋白酶的核酸构建体

    公开(公告)号:US5621089A

    公开(公告)日:1997-04-15

    申请号:US434255

    申请日:1995-05-03

    CPC分类号: C11D3/386 C12N15/75 C12N9/54

    摘要: The invention is directed to an isolated nucleic acid construct(s) comprising a nucleic acid sequence encoding an alkaline protease having a molecular weight of about 34 kD and is obtainable from a strain of Bacillus sp. Group I, as well as recombinant vectors and host cells comprising such constructs. Additionally, the invention is directed to a method for obtaining the protease. The invention is also directed to promoter and signal sequences derived from said alkaline protease as well as methods of using the promoter and signal sequences in the expression of heterologous nucleic acid sequences encoding heterologous proteins in bacteria.

    摘要翻译: 本发明涉及分离的核酸构建体,其包含编码分子量为约34kD的碱性蛋白酶的核酸序列,并且可从芽孢杆菌属菌株获得。 组I,以及包含这种构建体的重组载体和宿主细胞。 另外,本发明涉及获得蛋白酶的方法。 本发明还涉及来自所述碱性蛋白酶的启动子和信号序列以及在编码细菌中的异源蛋白质的异源核酸序列的表达中使用启动子和信号序列的方法。

    Recombinant methods for the production of a bacillus alkaline protease
    4.
    发明授权
    Recombinant methods for the production of a bacillus alkaline protease 失效
    用于生产杆菌碱性蛋白酶的重组方法

    公开(公告)号:US5622850A

    公开(公告)日:1997-04-22

    申请号:US460327

    申请日:1995-06-02

    CPC分类号: C11D3/386 C12N15/75 C12N9/54

    摘要: The invention is directed to an isolated nucleic acid construct(s) comprising a nucleic acid sequence encoding an alkaline protease having a molecular weight of about 34 kD and is obtainable from a strain of Bacillus sp. Group I, as well as recombinant vectors and host cells comprising such constructs. Additionally, the invention is directed to a method for obtaining the protease. The invention is also directed to promoter and signal sequences derived from said alkaline protease as well as methods of using the promoter and signal sequences in the expression of heterologous nucleic acid sequences encoding heterologous proteins in bacteria.

    摘要翻译: 本发明涉及分离的核酸构建体,其包含编码分子量为约34kD的碱性蛋白酶的核酸序列,并且可从芽孢杆菌属菌株获得。 组I,以及包含这种构建体的重组载体和宿主细胞。 另外,本发明涉及获得蛋白酶的方法。 本发明还涉及来自所述碱性蛋白酶的启动子和信号序列以及在编码细菌中的异源蛋白质的异源核酸序列的表达中使用启动子和信号序列的方法。

    Proteases
    9.
    发明授权
    Proteases 失效
    蛋白酶

    公开(公告)号:US5362414A

    公开(公告)日:1994-11-08

    申请号:US107689

    申请日:1993-08-18

    CPC分类号: C12N9/54 C11D3/386

    摘要: This invention is in the field of detergent proteases derived from strains of a new Bacillus sp. More specifically, the invention is directed towards a protease derived from a strain of Bacillus sp. TY 145. Moreover, the invention is directed towards a process for the preparation of the protease the use of the protease enzyme, and detergent composition comprising the protease of the invention.

    摘要翻译: PCT No.PCT / DK92 / 00104 Sec。 371日期:1993年8月18日 102(e)日期1993年8月18日PCT提交1992年4月3日PCT公布。 第WO92 / 17577号公报 日期为1992年10月15日。本发明涉及从新芽孢杆菌属菌株衍生的洗涤剂蛋白酶领域。 更具体地,本发明涉及来自芽孢杆菌属菌株的蛋白酶。 TY 145.此外,本发明涉及制备蛋白酶的方法,所述蛋白酶的用途以及包含本发明的蛋白酶的洗涤剂组合物。