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    G-beta-gamma regulated phosphatidylinositol-3' kinase
    1.
    发明授权
    G-beta-gamma regulated phosphatidylinositol-3' kinase 有权
    G-β-gamma调节磷脂酰肌醇-3'激酶

    公开(公告)号：US6017763A

    公开(公告)日：2000-01-25

    申请号：US225170

    申请日：1999-01-04

    申请人： Len Stephens ,  Phillip Thomas Hawkins ,  Sylvia Braselmann

    发明人： Len Stephens ,  Phillip Thomas Hawkins ,  Sylvia Braselmann

    IPC分类号： C12N15/02 ,  A61K31/00 ,  A61K31/70 ,  A61K31/7088 ,  A61K35/74 ,  A61K38/00 ,  A61K39/395 ,  A61K45/00 ,  A61K48/00 ,  A61P7/00 ,  A61P9/00 ,  A61P9/10 ,  A61P11/00 ,  A61P11/06 ,  A61P19/00 ,  A61P19/02 ,  A61P25/28 ,  A61P29/00 ,  A61P35/00 ,  A61P37/00 ,  A61P37/08 ,  A61P39/00 ,  A61P39/06 ,  A61P43/00 ,  C07K16/40 ,  C07K19/00 ,  C12N9/12 ,  C12N15/09 ,  C12N15/54 ,  C12P21/02 ,  C12Q1/48 ,  C12Q1/68 ,  C07H21/04 ,  C07K1/00

    CPC分类号： C12N9/1205 ,  C07K2319/00 ,  G01N2333/91215 ,  G01N2500/10 ,  Y10S530/829

    摘要： The present invention relates to the discovery, identification and characterization of nucleotides that encode the G protein regulated phosphatidylinositol-3' kinase, a heterodimeric enzyme which produces the intracellular messenger phosphatidylinositol (3,4,5)-triphosphate in response to activation of trimeric G protein-linked receptors. This novel protein, comprised of a catalytic subunit, p120, and a regulatory subunit, p101, is found in cells of hematopoietic origin and is involved in immune system responses which cause inflammation. The presence of p101 subunit is largely responsible for the dramatic stimulation of kinase activity in the presence of activated trimeric G proteins. The invention encompasses p101 and p120 nucleotides; host cell expression systems, p101 and p120 proteins, fusion proteins, polypeptides and peptides, antibodies to these proteins, transgenic animals that express a p101 or p120 transgene, or recombinant knock-out cells and animals that do not express the p101 or p120 gene, antagonists and agonists of the enzyme, and other compounds that modulate p101 or p120 gene expression or enzyme activity that can be used for diagnosis, drug screening, clinical trial monitoring, and/or the treatment of inflammatory disorders.

    摘要翻译： 本发明涉及编码G蛋白调节的磷脂酰肌醇-3'激酶的核苷酸的发现，鉴定和表征，其是响应于三聚体G的活化而产生细胞内信使磷脂酰肌醇（3,4,5） - 三磷酸的异源二聚体 蛋白质连接的受体。 这种由催化亚单位p120和调节亚基p101组成的新蛋白质在造血起源的细胞中发现，并且涉及引起炎症的免疫系统反应。 p101亚单位的存在主要是由于在活化的三聚体G蛋白的存在下剧烈刺激激酶活性。 本发明包括p101和p120核苷酸; 宿主细胞表达系统，p101和p120蛋白，融合蛋白，多肽和肽，对这些蛋白质的抗体，表达p101或p120转基因的转基因动物，或不表达p101或p120基因的重组敲除细胞和动物， 酶的拮抗剂和激动剂，以及调节可以用于诊断，药物筛选，临床试验监测和/或治疗炎性疾病的p101或p120基因表达或酶活性的其它化合物。

    Nucleotide sequences that encode phosphatidylinositol-3′ kinase associated proteins and uses thereof
    3.
    发明授权
    Nucleotide sequences that encode phosphatidylinositol-3′ kinase associated proteins and uses thereof 失效
    编码磷脂酰肌醇-3'激酶相关蛋白的核苷酸序列及其用途

    公开(公告)号：US06762284B1

    公开(公告)日：2004-07-13

    申请号：US09687230

    申请日：2000-10-13

    申请人： Sylvia Braselmann

    发明人： Sylvia Braselmann

    IPC分类号： C07K1700

    CPC分类号： C12N9/1205 ,  A61K38/00 ,  C07K14/47 ,  C07K2319/00

    摘要： Identification, characterization and expression of nucleotides that encode phosphatidylinositol-3′kinase associated protein(s) that bind to the intermediate SH2 domain on the regulatory subunit of PI3K, p85, by the associated protein(s) C-terminal amino acids, and that exhibit a bromodomain are described, as well as methods of using such proteins for medical applications, including diagnosis and treatment cell growth disorders.

    摘要翻译： 编码磷脂酰肌醇-3'-激酶相关蛋白的核苷酸的鉴定，表征和表达，其结合相关蛋白C端氨基酸在PI3K，p85的调节亚单位上的中间体SH2结构域，并且 描述了溴结构域，以及使用这些蛋白质用于医学应用的方法，包括诊断和治疗细胞生长障碍。