公开(公告)号：US07241988B2

公开(公告)日：2007-07-10

申请号：US10630904

申请日：2003-07-31

申请人： Lewis Gruber ,  Kenneth Bradley ,  Ward Lopes ,  Robert W. Lancelot ,  Joseph S. Plewa ,  David G. Grier

发明人： Lewis Gruber ,  Kenneth Bradley ,  Ward Lopes ,  Robert W. Lancelot ,  Joseph S. Plewa ,  David G. Grier

IPC分类号： G01N21/63

CPC分类号： G01N15/1459 ,  B01L3/5027 ,  G01N2015/149 ,  G02B21/32 ,  G03H1/08 ,  G03H1/2294 ,  G03H2001/0077 ,  G03H2001/085 ,  G03H2225/32 ,  H05H3/04

摘要： The present invention employs a beam steering apparatus to isolate valuable cells from other cells, tissues, and contaminants. In one embodiment, the system balances optical trapping against biasing flow to parallelize cell sorting under the flexible control of computer program-directed traps which differentially manipulate cells based on their composition or labels to direct separation.

摘要翻译： 本发明采用光束转向装置来将有价值的细胞与其它细胞，组织和污染物分离。 在一个实施例中，系统平衡光学捕获与偏置流平衡，以在计算机程序导向的陷阱的灵活控制下平行化细胞分选，其基于它们的组成或标签差异地操纵细胞以引导分离。

公开(公告)号：US20110091932A1

公开(公告)日：2011-04-21

申请号：US12926888

申请日：2010-12-15

申请人： Joseph S. Plewa ,  Evan B. Tanner ,  Daniel M. Mueth ,  Crystal J. Duke ,  Kevin J. Ehrenreich

发明人： Joseph S. Plewa ,  Evan B. Tanner ,  Daniel M. Mueth ,  Crystal J. Duke ,  Kevin J. Ehrenreich

IPC分类号： C12Q1/04 ,  C12Q1/02

CPC分类号： G02B21/32 ,  B01L3/502761 ,  G01N1/34 ,  G01N15/1463 ,  G01N2001/007 ,  G01N2015/1454 ,  G01N2015/149 ,  G02B5/32 ,  Y10T436/25375

摘要： An apparatus and method for purifying a forensic sample using an automated extraction and purification system includes optical tweezers; an input channel through which the sample is introduced; a chamber which receives the sample from the input channel; a collection channel through which selected particles of the sample are removed; and an output through which unselected particles of the sample are removed. At least one buffer input channel is provided. The input channel may allow sedimentation of the sample into the chamber by gravity. In another arrangement, the system includes an optical trapping system; a first channel through which the sample is introduced; a second channel through which buffer is introduced; wherein the optical tweezers are used to move selected particles of the sample from the first channel to the second channel. The selected particles may be sperm. The optical tweezers preferably utilize holographic optical trapping.

公开(公告)号：US07875845B2

公开(公告)日：2011-01-25

申请号：US11298565

申请日：2005-12-12

申请人： Joseph S. Plewa ,  Evan B. Tanner ,  Daniel M. Mueth ,  Crystal J. Duke ,  Kevin J. Ehrenreich

发明人： Joseph S. Plewa ,  Evan B. Tanner ,  Daniel M. Mueth ,  Crystal J. Duke ,  Kevin J. Ehrenreich

IPC分类号： H01S1/00 ,  C12Q1/00 ,  G01N1/18 ,  G01N21/00

CPC分类号： G02B21/32 ,  B01L3/502761 ,  G01N1/34 ,  G01N15/1463 ,  G01N2001/007 ,  G01N2015/1454 ,  G01N2015/149 ,  G02B5/32 ,  Y10T436/25375

摘要： An apparatus and method for purifying a forensic sample using an automated extraction and purification system includes optical tweezers; an input channel through which the sample is introduced; a chamber which receives the sample from the input channel; a collection channel through which selected particles of the sample are removed; and an output through which unselected particles of the sample are removed. At least one buffer input channel is provided. The input channel may allow sedimentation of the sample into the chamber by gravity. In another arrangement, the system includes an optical trapping system; a first channel through which the sample is introduced; a second channel through which buffer is introduced; wherein the optical tweezers are used to move selected particles of the sample from the first channel to the second channel. The selected particles may be sperm. The optical tweezers preferably utilize holographic optical trapping.

摘要翻译： 使用自动提取和净化系统来净化法医样品的装置和方法包括光学镊子; 导入样品的输入通道; 从输入通道接收样品的室; 取出样品的所选粒子的收集通道; 以及去除样品的未选择的颗粒的输出。 提供至少一个缓冲器输入通道。 输入通道可以允许样品通过重力沉降到室中。 在另一种布置中，该系统包括光学捕获系统; 引入样品的第一通道; 引入缓冲液的第二通道; 其中所述光学镊子用于将所述样品的选定颗粒从所述第一通道移动到所述第二通道。 所选择的颗粒可能是精子。 光学镊子优选利用全息光学捕获。