公开(公告)号：US20240280464A1

公开(公告)日：2024-08-22

申请号：US18628729

申请日：2024-04-07

申请人： Cytek Biosciences, Inc.

发明人： David Vrane

IPC分类号： G01N15/1404 ,  G01N15/10 ,  G01N15/1409

CPC分类号： G01N15/1404 ,  G01N2015/1006 ,  G01N15/1409

摘要： A pressure regulated fluidics system of a flow cytometer includes a sheath tank holding sheath fluid; a degasser coupled to the sheath tank by a first sheath line; a manifold assembly coupled to the degasser by a second sheath line; a first valve coupled to the second sheath line; a pressure regulator coupled to the sheath tank; and a transducer coupled to and between the pressure regulator and the first valve. The transducer senses measured pressure and converts it into a voltage. The pressure regulator applies regulated pressure to the sheath fluid to maintain a total flow rate of fluids through the flow cytometer based on the voltage. The degasser pulls gas molecules out of the sheath fluid.

公开(公告)号：US11946848B2

公开(公告)日：2024-04-02

申请号：US17984116

申请日：2022-11-09

申请人： Iris International, Inc.

发明人： Thomas H. Adams ,  Bart J. Wanders ,  John Roche ,  Harvey L. Kasdan

IPC分类号： G01N15/14 ,  G01N1/30 ,  G01N15/00 ,  G01N15/06 ,  G01N15/10 ,  G01N15/1404 ,  G01N15/1429 ,  G01N15/1434 ,  G01N21/05 ,  G01N21/53 ,  G01N33/49 ,  G01N33/50 ,  G01N33/80 ,  G02B7/28 ,  G02B7/36 ,  G02B21/24 ,  G06T7/00 ,  G01N15/1409

CPC分类号： G01N15/1404 ,  G01N1/30 ,  G01N15/06 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1436 ,  G01N15/1459 ,  G01N15/1463 ,  G01N15/1468 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/53 ,  G01N33/49 ,  G01N33/4915 ,  G01N33/5091 ,  G01N33/5094 ,  G01N33/80 ,  G02B7/36 ,  G01N2015/0065 ,  G01N2015/1037 ,  G01N2015/1411 ,  G01N2015/1413 ,  G01N2015/144 ,  G01N2015/1452 ,  G01N2015/1481 ,  G01N2015/1486 ,  G01N2021/058 ,  G02B7/28 ,  G02B21/244 ,  G06T7/0012 ,  G06T2207/10148

摘要： For analyzing a sample containing particles of at least two categories, such as a sample containing blood cells, a particle counter subject to a detection limit is coupled with an analyzer capable of discerning particle number ratios, such as a visual analyzer, and a processor. A first category of particles can be present beyond detection range limits while a second category of particles is present within respective detection range limits. The concentration of the second category of particles is determined by the particle counter. A ratio of counts of the first category to the second category is determined on the analyzer. The concentration of particles in the first category is calculated on the processor based on the ratio and the count or concentration of particles in the second category.

公开(公告)号：US20240293815A1

公开(公告)日：2024-09-05

申请号：US17617545

申请日：2021-10-18

申请人： FUDAN UNIVERSITY

发明人： Antoine Jean-Pierre Rene RIAUD ,  Qing WANG ,  Jia ZHOU

IPC分类号： B01L3/00 ,  G01N15/14 ,  G01N15/1404 ,  G01N15/1409

CPC分类号： B01L3/502761 ,  B01L3/50273 ,  G01N15/1404 ,  G01N15/1409 ,  G01N15/1425 ,  B01L2200/0668 ,  B01L2400/0436 ,  G01N2015/1415

摘要： The invention discloses a method and an apparatus for trapping and manipulating particles, relating to the field of acoustic manipulation. The invention discloses a photoacoustic tweezers, which use bimodal transducer generate a weak acoustic wave and a strong acoustic wave. The strong acoustic wave behaves as an effective gain medium to amplify the acoustic radiation force of this weak acoustic wave. After an interference of the strong acoustic wave and the weak acoustic wave, an amplified trapping force is produced for achieving the trapping and manipulation of particles. The invention can manipulate several particles simultaneously in high throughput and particles of various sizes ranging from 1 μm to 1 mm. The invention can form reconfigurable acoustic fields which permits versatile and selective manipulation with no need of complicated acoustic arrays. The invention is a versatile, biocompatible, selective and high-throughput manipulation method.

公开(公告)号：US20240278240A1

公开(公告)日：2024-08-22

申请号：US18632183

申请日：2024-04-10

申请人： Cytek Biosciences, Inc.

发明人： Glen Krueger ,  David Vrane ,  Kuncheng Wang ,  Qiuta Gu

IPC分类号： B01L3/00 ,  C12M1/00 ,  G01N15/01 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1409 ,  G01N15/1434 ,  G01N15/149

CPC分类号： B01L3/502761 ,  B01L3/502715 ,  C12M47/04 ,  G01N15/14 ,  G01N15/1436 ,  B01L2300/0654 ,  B01L2300/0663 ,  B01L2300/0681 ,  B01L2300/0829 ,  B01L2300/1805 ,  B01L2300/1894 ,  G01N15/01 ,  G01N2015/1006 ,  G01N2015/1028 ,  G01N15/1409 ,  G01N15/149

摘要： Methods for a sorting flow cytometer system are disclosed. The method includes forming a stream of a sample biological fluid; charging the stream of the sample biological fluid with a first voltage having a first voltage polarity; breaking a first charged drop off of the stream, wherein the first charged drop is charged with the first voltage; dropping the first charged drop between a first electrostatic charge plate and a second electrostatic charge plate opposite the first electrostatic charge plate, wherein the first electrostatic charge plate and the second electrostatic charge plate are positioned on an angle to form a progressively larger gap between each; deflecting the first charged drop off of a center stream path towards the first electrostatic charge plate to drop along a line with a first angle; and orienting a first pivotal scupper to allow the first charged drop to fall into a first test tube.

公开(公告)号：US20240241029A1

公开(公告)日：2024-07-18

申请号：US18409900

申请日：2024-01-11

申请人： SYSMEX CORPORATION

发明人： Masatsugu OZASA ,  Motoi KINISHI ,  Clare WEIR

IPC分类号： G01N15/1409 ,  G01N15/075

CPC分类号： G01N15/1409 ,  G01N15/075

摘要： Disclosed is a flow cytometer comprising: a flow cell; a sample supply part configured to supply, to the flow cell, a predetermined amount of a measurement sample prepared by mixing a specimen containing measurement-target substances and a labeled antibody which binds to the measurement-target substances; a light source configured to apply light to the measurement sample passing through the flow cell; a detector configured to detect lights derived from the measurement-target substances contained in the measurement sample to which the light has been applied, and output signals; and an analysis unit configured to count the measurement-target substances contained in the measurement sample on the basis of the signals and obtain a concentration of the measurement-target substances on the basis of the number of the counted measurement-target substances and the predetermined amount.

公开(公告)号：US20240361229A1

公开(公告)日：2024-10-31

申请号：US18688322

申请日：2021-09-03

申请人： SARTORIUS BIOANALYTICAL INSTRUMENTS, INC.

发明人： Edward MORRELL ,  Heinrich FELDOTTE ,  Richard ESSER

IPC分类号： G01N15/1409 ,  G01N15/1434

CPC分类号： G01N15/1409 ,  G01N15/1434

摘要： A flow cytometry evaluation system includes a sample effluent system with an effluent collection vessel with an effluent fluid inlet to receive an effluent of the fluid sample exiting the investigation zone during a flow cytometry evaluation; and an effluent fluid conduction path from the investigation zone to the effluent fluid inlet. A pressurized gas delivery system in fluid communication with the sample effluent system applies pressurized gas to the fluid sample effluent system to impede fluid flow through the effluent fluid conduction path toward the effluent fluid inlet during a flow cytometry investigation

公开(公告)号：US20240192116A1

公开(公告)日：2024-06-13

申请号：US18555345

申请日：2022-04-14

申请人： SCREENSYS GMBH

发明人： Alexander GROSS ,  Jialan CAO ,  Johann Michael K¿HLER ,  Steffen SCHNEIDER ,  Patrick SCHAUB ,  Klaus PALME

IPC分类号： G01N15/1409 ,  B01L3/00 ,  G01N35/08

CPC分类号： G01N15/1409 ,  B01L3/502761 ,  G01N35/08 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2400/082

摘要： System and method for handling dispersed microscopic objects contained in a sample fluid, the system comprising a first microfluidic device comprising a microchannel with an inlet, an outlet and an opening, the opening being located between the inlet and the outlet, a conveying device configured to pump a carrier fluid via the inlet into the microchannel with an input volumetric flow rate and to remove fluid from the microchannel via the outlet with an output volumetric flow rate, a first sensor unit for identifying positions of the dispersed objects in the sample fluid, and a positioning unit configured to position the opening at a target position proximate the position of a target object.

公开(公告)号：US20240183771A1

公开(公告)日：2024-06-06

申请号：US18574608

申请日：2021-07-30

申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften E.V.

发明人： Elena Erben ,  Nicola Maghelli ,  Moritz Kreysing

IPC分类号： G01N15/1409 ,  B01L3/00 ,  G01N15/1434 ,  G02B21/32 ,  G01N15/1404

CPC分类号： G01N15/1409 ,  B01L3/502761 ,  G01N15/1434 ,  G02B21/32 ,  B01L2200/0663 ,  B01L2200/0668 ,  B01L2200/143 ,  B01L2300/1872 ,  B01L2400/0445 ,  B01L2400/0451 ,  G01N2015/1413

摘要： The invention concerns a method for spatially manipulating at least one particle in a fluid, wherein the particle or the particles is/are spatially manipulated in the fluid by hydrodynamic flows which are generated in the fluid by means of dynamic localized heating of the fluid. The method according to the invention is characterized in that at least one target spatial configuration of the particle(s) in the fluid is defined and that the following further steps are carried out: a) an actual spatial configuration of the particle(s) is captured, b) a specific dynamic localized heating event to be applied to the fluid is determined in dependence of at least one recent actual spatial configuration of the particle(s) and a target configuration of the particle(s), c) the specific dynamic localized heating as determined in step c) is applied at least once to the fluid and d) at least one or all of the steps a) to c) are repeated. The invention concerns furthermore an apparatus for spatially manipulating at least one particle in a fluid by means of hydrodynamic flows a computer program product and a computer-readable storage medium.

公开(公告)号：US12105008B2

公开(公告)日：2024-10-01

申请号：US17790955

申请日：2021-11-30

申请人： Beckman Coulter, Inc. ,  Beckman Coulter Biotechnology (Suzhou) Co., Ltd.

发明人： Linqun Tang ,  Simon Nava ,  Juntao Yuan ,  Liang Shi

IPC分类号： G01N15/1404 ,  B01L3/00 ,  G01N15/1409

CPC分类号： G01N15/1404 ,  B01L3/502 ,  B01L2200/146 ,  B01L2300/049 ,  B01L2300/14 ,  B01L2400/049 ,  B01L2400/0622 ,  G01N15/1409

摘要： The present disclosure relates to a fluid system for a sample processor and a sample processor including the fluid system. The fluid system includes a sample line, a processing fluid line, a vacuum line, and an air pump. The sample line communicates a sample container with a sample port of a flow cell unit. The processing fluid line communicates a sheath fluid container with a processing fluid port of the flow cell unit. The vacuum line is in communication with the flow cell unit. The air pump includes a first output port and a second output port. Pressurized gas is generated at the first output port, and the first output port is in communication with the sample container and the sheath fluid container. A vacuum is generated at the second output port, and the second output port is in communication with a vacuum port of the flow cell unit through the vacuum line.

公开(公告)号：US20240295484A1

公开(公告)日：2024-09-05

申请号：US18582156

申请日：2024-02-20

申请人： The University of Hong Kong

发明人： Kevin Kin Man TSIA ,  Chak Man LEE

IPC分类号： G01N15/1409 ,  G01N15/10 ,  G01N15/14 ,  G01N15/1404 ,  G01N15/149

CPC分类号： G01N15/1409 ,  G01N2015/1006 ,  G01N2015/1413 ,  G01N15/149 ,  G01N2015/1493

摘要： A high-throughput single-file focusing system and methods for polydisperse particles are provided. The system includes a microfluidic device for pre-focusing the polydisperse particles and a high-aspect-ratio (HAR) rectangular structure coupled to the microfluidic device. The microfluidic device includes a fluidic channel configured to localize distributions of the polydisperse particles in a cross-sectional area of the fluidic channel. The dimensions of the fluidic channel are configured to generate a converging secondary flow having four spiral vortices that drives the polydisperse particles to flow inward following a spiral path to be concentrated into a center of each spiral vortex such that the polydisperse particles are focused by the converging secondary flow without any inertial force. The coupled HAR rectangular structure receives the pre-focused polydisperse particles and further confines the particles to form a single file on its mid-plane.