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公开(公告)号:US20050153355A1
公开(公告)日:2005-07-14
申请号:US11059035
申请日:2005-02-14
申请人: Linda Lee , Ronald Graham , William Werner , Elana Swartzman , Lily Lu
发明人: Linda Lee , Ronald Graham , William Werner , Elana Swartzman , Lily Lu
IPC分类号: G01N33/53 , C07D311/80 , C07D491/04 , C07D491/06 , C07H19/207 , C07H21/00 , C07K1/13 , C09B11/24 , C09B11/28 , C09K11/06 , C12N15/09 , C12Q1/68 , G01N33/533 , G01N33/543 , G01N33/58 , C07D491/14 , C07H19/04 , C07H21/04 , C07D45/14
CPC分类号: C07D491/04 , C07D311/80 , C07D491/06 , C07H19/207 , C07H21/00 , C09B11/24 , G01N33/582 , G01N2458/00 , Y10S436/80
摘要: The present invention provides novel, water-soluble, red-emitting fluorescent rhodamine dyes and red-emitting fluorescent energy-transfer dye pairs, as well as labeled conjugates comprising the same and methods for their use. The dyes, energy-transfer dye pairs and labeled conjugates are useful in a variety of aqueous-based applications, particularly in assays involving staining of cells, protein binding, and/or analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing.
摘要翻译: 本发明提供新颖的水溶性红色荧光罗丹明染料和红色发光荧光能量转移染料对,以及包含其的标记共轭物及其使用方法。 染料,能量转移染料对和标记的缀合物可用于各种水基应用,特别是涉及细胞染色,蛋白质结合和/或核酸分析的测定,如杂交测定和核酸测序。
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公开(公告)号:US06191278B1
公开(公告)日:2001-02-20
申请号:US09433093
申请日:1999-11-03
申请人: Linda G. Lee , Ronald J. Graham , William E. Werner , Elana Swartzman , Lily Lu
发明人: Linda G. Lee , Ronald J. Graham , William E. Werner , Elana Swartzman , Lily Lu
IPC分类号: C07D49304
CPC分类号: C07D491/04 , C07D311/80 , C07D491/06 , C07H19/207 , C07H21/00 , C09B11/24 , G01N33/582 , G01N2458/00
摘要: The present invention provides novel, water-soluble, red-emitting fluorescent rhodamine dyes and red-emitting fluorescent energy-transfer dye pairs, as well as labeled conjugates comprising the same and methods for their use. The dyes, energy-transfer dye pairs and labeled conjugates are useful in a variety of aqueous-based applications, particularly in assays involving staining of cells, protein binding, and/or analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing.
摘要翻译: 本发明提供新颖的水溶性红色荧光罗丹明染料和红色发光荧光能量转移染料对,以及包含其的标记共轭物及其使用方法。 染料,能量转移染料对和标记的缀合物可用于各种水基应用,特别是涉及细胞染色,蛋白质结合和/或核酸分析的测定,如杂交测定和核酸测序。
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公开(公告)号:US07504496B2
公开(公告)日:2009-03-17
申请号:US11059035
申请日:2005-02-14
申请人: Linda G. Lee , Ronald J. Graham , William E. Werner , Elana Swartzman , Lily Lu
发明人: Linda G. Lee , Ronald J. Graham , William E. Werner , Elana Swartzman , Lily Lu
CPC分类号: C07D491/04 , C07D311/80 , C07D491/06 , C07H19/207 , C07H21/00 , C09B11/24 , G01N33/582 , G01N2458/00 , Y10S436/80
摘要: The present invention provides novel, water-soluble, red-emitting fluorescent rhodamine dyes and red-emitting fluorescent energy-transfer dye pairs, as well as labeled conjugates comprising the same and methods for their use. The dyes, energy-transfer dye pairs and labeled conjugates are useful in a variety of aqueous-based applications, particularly in assays involving staining of cells, protein binding, and/or analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing.
摘要翻译: 本发明提供新颖的水溶性红色荧光罗丹明染料和红色发光荧光能量转移染料对,以及包含其的标记共轭物及其使用方法。 染料,能量转移染料对和标记的缀合物可用于各种水基应用,特别是涉及细胞染色,蛋白质结合和/或核酸分析的测定,如杂交测定和核酸测序。
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公开(公告)号:US20110081688A1
公开(公告)日:2011-04-07
申请号:US12855649
申请日:2010-08-12
申请人: Linda G. Lee , Ronald J. Graham , William E. Werner , Elana Swartzman , Lily Lu
发明人: Linda G. Lee , Ronald J. Graham , William E. Werner , Elana Swartzman , Lily Lu
IPC分类号: C12P19/34 , C07H19/04 , C07D498/14 , C07D498/22
CPC分类号: C07D491/04 , C07D311/80 , C07D491/06 , C07H19/207 , C07H21/00 , C09B11/24 , G01N33/582 , G01N2458/00 , Y10S436/80
摘要: The present invention provides novel, water-soluble, red-emitting fluorescent rhodamine dyes and red-emitting fluorescent energy-transfer dye pairs, as well as labeled conjugates comprising the same and methods for their use. The dyes, energy-transfer dye pairs and labeled conjugates are useful in a variety of aqueous-based applications, particularly in assays involving staining of cells, protein binding, and/or analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing.
摘要翻译: 本发明提供新颖的水溶性红色荧光罗丹明染料和红色发光荧光能量转移染料对,以及包含其的标记共轭物及其使用方法。 染料,能量转移染料对和标记的缀合物可用于各种水基应用,特别是涉及细胞染色,蛋白质结合和/或核酸分析的测定,如杂交测定和核酸测序。
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公开(公告)号:US07795042B2
公开(公告)日:2010-09-14
申请号:US12402634
申请日:2009-03-12
申请人: Linda G. Lee , Ronald J. Graham , William E. Werner , Elana Swartzman , Lily Lu
发明人: Linda G. Lee , Ronald J. Graham , William E. Werner , Elana Swartzman , Lily Lu
CPC分类号: C07D491/04 , C07D311/80 , C07D491/06 , C07H19/207 , C07H21/00 , C09B11/24 , G01N33/582 , G01N2458/00 , Y10S436/80
摘要: The present invention provides novel, water-soluble, red-emitting fluorescent rhodamine dyes and red-emitting fluorescent energy-transfer dye pairs, as well as labeled conjugates comprising the same and methods for their use. The dyes, energy-transfer dye pairs and labeled conjugates are useful in a variety of aqueous-based applications, particularly in assays involving staining of cells, protein binding, and/or analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing.
摘要翻译: 本发明提供新颖的水溶性红色荧光罗丹明染料和红色发光荧光能量转移染料对,以及包含其的标记共轭物及其使用方法。 染料,能量转移染料对和标记的缀合物可用于各种水基应用,特别是涉及细胞染色,蛋白质结合和/或核酸分析的测定,如杂交测定和核酸测序。
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公开(公告)号:US06372907B1
公开(公告)日:2002-04-16
申请号:US09661206
申请日:2000-09-14
申请人: Linda G. Lee , Ronald J. Graham , William E. Werner , Elana Swartzman , Lily Lu
发明人: Linda G. Lee , Ronald J. Graham , William E. Werner , Elana Swartzman , Lily Lu
IPC分类号: C07D40500
CPC分类号: C07D491/04 , C07D311/80 , C07D491/06 , C07H19/207 , C07H21/00 , C09B11/24 , G01N33/582 , G01N2458/00 , Y10S436/80
摘要: The present invention provides novel, water-soluble, red-emitting fluorescent rhodamine dyes and red-emitting fluorescent energy-transfer dye pairs, as well as labeled conjugates comprising the same and methods for their use. The dyes, energy-transfer dye pairs and labeled conjugates are useful in a variety of aqueous-based applications, particularly in assays involving staining of cells, protein binding, and/or analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing.
摘要翻译: 本发明提供新颖的水溶性红色荧光罗丹明染料和红色发光荧光能量转移染料对,以及包含其的标记共轭物及其使用方法。 染料,能量转移染料对和标记的缀合物可用于各种水基应用,特别是涉及细胞染色,蛋白质结合和/或核酸分析的测定,如杂交测定和核酸测序。
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公开(公告)号:US08030096B2
公开(公告)日:2011-10-04
申请号:US12855649
申请日:2010-08-12
申请人: Linda G. Lee , Ronald Graham , Lily Lu , Elana E. Swartzman , William E. Werner
发明人: Linda G. Lee , Ronald Graham , Lily Lu , Elana E. Swartzman , William E. Werner
CPC分类号: C07D491/04 , C07D311/80 , C07D491/06 , C07H19/207 , C07H21/00 , C09B11/24 , G01N33/582 , G01N2458/00 , Y10S436/80
摘要: The present invention provides novel, water-soluble, red-emitting fluorescent rhodamine dyes and red-emitting fluorescent energy-transfer dye pairs, as well as labeled conjugates comprising the same and methods for their use. The dyes, energy-transfer dye pairs and labeled conjugates are useful in a variety of aqueous-based applications, particularly in assays involving staining of cells, protein binding, and/or analysis of nucleic acids, such as hybridization assays and nucleic acid sequencing.
摘要翻译: 本发明提供新颖的水溶性红色荧光罗丹明染料和红色发光荧光能量转移染料对,以及包含其的标记共轭物及其使用方法。 染料,能量转移染料对和标记的缀合物可用于各种水基应用,特别是涉及细胞染色,蛋白质结合和/或核酸分析的测定,如杂交测定和核酸测序。
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公开(公告)号:US08464503B2
公开(公告)日:2013-06-18
申请号:US12891584
申请日:2010-09-27
申请人: Frank W. McCuin , Shapour Bakhtiari , Clinton Johnson , Charles C. McCuin , Eric Morton , Lily Lu
发明人: Frank W. McCuin , Shapour Bakhtiari , Clinton Johnson , Charles C. McCuin , Eric Morton , Lily Lu
IPC分类号: A01D69/00
CPC分类号: A01D34/82
摘要: An engine comprises a tank of compressed air and a piston. The tank of compressed air is connected to a conduit and the conduit is connected to a cylinder comprising a first valve and an inlet to a chamber. The chamber comprises the piston, which reciprocates. The chamber also has a second outlet connected to a second valve. The air traverses from the tank of compressed into the chamber by opening the first valve. Both the first valve and second valve are closed and the compressed air moves the piston in the chamber to produce work. The second valve opens and the air escapes from the chamber and the piston.
摘要翻译: 发动机包括压缩空气罐和活塞。 压缩空气罐连接到管道,并且管道连接到包括第一阀和到室的入口的气缸。 该室包括往复运动的活塞。 该室还具有连接到第二阀的第二出口。 通过打开第一个阀,空气从被压缩的容器中流出。 第一阀门和第二阀门都关闭,压缩空气将活塞移动到腔室中以产生作业。 第二个阀打开,空气从室和活塞中逸出。
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公开(公告)号:US20060292639A1
公开(公告)日:2006-12-28
申请号:US11201937
申请日:2005-08-11
申请人: Per-Erik Mansson , Diane DeHaven-Hudkins , Lily Lu
发明人: Per-Erik Mansson , Diane DeHaven-Hudkins , Lily Lu
IPC分类号: G01N33/567 , C07H21/04 , C12P21/06 , C07K14/705 , G01N33/53
CPC分类号: G01N33/566 , C07K14/705 , G01N2333/705 , G01N2500/04
摘要: The invention provides a splice variant of the vanilloid receptor termed “VR1A.” VR1A has a unique N-terminus as compared with wild type VR1. The invention provides nucleic acid sequences encoding VR1A, expression vectors for expressing VR1A, amino acid sequences of VR1A, antibodies against VR1A and methods of making and using the polynucleotides, polypeptides and antibodies of the invention.
摘要翻译: 本发明提供称为“VR 1A”的香草素受体的剪接变体。 与野生型VR 1相比,VR 1A具有独特的N末端。 本发明提供编码VR 1A的核酸序列,用于表达VR 1A的表达载体,VR1A的氨基酸序列,针对VR 1A的抗体以及制备和使用本发明的多核苷酸,多肽和抗体的方法。
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公开(公告)号:US20120073280A1
公开(公告)日:2012-03-29
申请号:US12891584
申请日:2010-09-27
申请人: Frank W. McCuin , Shapour Bakhtian , Clinton Johnson , Charles C. McCuin , Eric Morton , Lily Lu
发明人: Frank W. McCuin , Shapour Bakhtian , Clinton Johnson , Charles C. McCuin , Eric Morton , Lily Lu
IPC分类号: F16D31/02
CPC分类号: A01D34/82
摘要: An engine comprises a tank of compressed air and a piston. The tank of compressed air is connected to a conduit and the conduit is connected to a cylinder comprising a first valve and an inlet to a chamber. The chamber comprises the piston, which reciprocates. The chamber also has a second outlet connected to a second valve. The air traverses from the tank of compressed into the chamber by opening the first valve. Both the first valve and second valve are closed and the compressed air moves the piston in the chamber to produce work. The second valve opens and the air escapes from the chamber and the piston.
摘要翻译: 发动机包括压缩空气罐和活塞。 压缩空气罐连接到管道,并且管道连接到包括第一阀和到室的入口的气缸。 该室包括往复运动的活塞。 该室还具有连接到第二阀的第二出口。 通过打开第一个阀,空气从被压缩的容器中流出。 第一阀门和第二阀门都关闭,压缩空气将活塞移动到腔室中以产生作业。 第二个阀打开,空气从室和活塞中逸出。
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