公开(公告)号：US09709479B2

公开(公告)日：2017-07-18

申请号：US14523376

申请日：2014-10-24

申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY

发明人： Marc Castellarnau ,  Gregory Lee Szeto ,  Darrell J. Irvine ,  John Christopher Love ,  Joel Voldman

IPC分类号： G01N15/10 ,  G01N15/14 ,  G01N35/00 ,  G01N15/00

CPC分类号： G01N15/1463 ,  G01N15/10 ,  G01N15/1484 ,  G01N35/00732 ,  G01N2015/0065 ,  G01N2015/008 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2035/00811 ,  G01N2035/00831

摘要： A method of tracking cell identity across analytical platforms uses stochastic barcoding (SB). SB uses a randomly generated code based on one or more of the number, color and position of beads encapsulated together with a set of cells of interest. SB use is demonstrated in an application where cells are transferred from a microwell array into a microtiter plate while keeping their identity, and obtained an average identification accuracy of 96% for transfer of 100 blocks. Model scaling of the method up to 1000 blocks demonstrated that SB is able to achieve approximately 90% accuracy.

公开(公告)号：US20150080234A1

公开(公告)日：2015-03-19

申请号：US14488269

申请日：2014-09-16

申请人： Massachusetts Institute of Technology

发明人： John Christopher Love ,  Ayca Yalcin Ozkumur ,  Brittany Anne Thomas

IPC分类号： G01N33/487

CPC分类号： G01N33/48735 ,  C12N5/0068 ,  C12N2533/30 ,  C12N2533/32 ,  C12N2533/50 ,  C12N2535/00 ,  C12N2537/00

摘要： The present disclosure provides technologies for achieving cell patterning, as well as patterned cell arrays achieved with such technologies. Provided apparatus and methods are useful in a variety of contexts and provide particular advantages with respect to assessing living cell arrays and/or their responsiveness to stimuli, including identifying and/or characterizing complex cellular responses.

摘要翻译： 本公开提供了用于实现细胞图案化的技术，以及用这种技术实现的图案化电池阵列。 提供的装置和方法在各种情况下是有用的，并且在评估活细胞阵列和/或其对刺激的响应性方面提供特别的优点，包括识别和/或表征复杂的细胞反应。

公开(公告)号：US20140307931A1

公开(公告)日：2014-10-16

申请号：US14253230

申请日：2014-04-15

申请人： Massachusetts Institute of Technology

发明人： Todd Michael Gierahn ,  Denis Loginov ,  John Christopher Love

IPC分类号： G06T7/00

CPC分类号： G06T7/11 ,  G06T2207/10056 ,  G06T2207/20068 ,  G06T2207/30072

摘要： A system for providing fully automated image segmentation and quality control of a protein array with no human interaction is presented. The system includes a memory and a processor configured by the memory. The processor is configured to receive an image file of the protein array, to geometrically distinguish a first block within the image, register a nominal location of the first block to the image by assigning a geometric code identifying the first block, draw a plurality of grid line on the array to delineate the first block from a second block, iteratively cluster a sub image for each feature in the delineated blocks to define a foreground area and a background area, identify pixels containing an artifact for each feature, and extract and return the signal intensity, variance, and quality of background and foreground pixels in each feature.

摘要翻译： 提出了一种能够提供全自动图像分割和蛋白质阵列质量控制的系统，无需人为的相互作用。 该系统包括由存储器配置的存储器和处理器。 处理器被配置为接收蛋白质阵列的图像文件，以几何地区分图像内的第一块，通过分配识别第一块的几何代码将第一块的标称位置注册到图像，绘制多个栅格 在第二个数据块上划分第一个数据块，对所划分的数据块中的每一个特征迭代地聚集一个子图像，以定义一个前景区域和一个背景区域，识别包含每个特征的工件的像素，并提取并返回 每个特征中的背景和前景像素的信号强度，方差和质量。