公开(公告)号：US20160060648A1

公开(公告)日：2016-03-03

申请号：US14939691

申请日：2015-11-12

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Edwards Allen ,  Veena S. Anil ,  Amarjit Basra ,  Christopher T. Bauer ,  Prasanna R. Bhat ,  Raghunatha P. Chari ,  Jaishree Chittoor ,  Paul Chomet ,  Karen K. Gabbert ,  Cara Griffith ,  Bill Hendrix ,  Alberto Iandolino ,  Hongwu Jia ,  Kevin R. Kosola ,  Saritha V. Kuriakose ,  Paul J. Loida ,  Linda L. Lutfiyya ,  Mingsheng Peng ,  Ying Peng ,  Bellur Narasimha Prasad ,  Monnanda S. Rajani ,  Monica P. Ravanello ,  Donald Todd ,  Srikanth Babu Venkatachalayya ,  Tyamagondlu V. Venkatesh ,  K. R. Vidya ,  Huai Wang

IPC: C12N15/82

CPC classification number: C12N15/8274 ,  C07K14/415 ,  C12N15/8271 ,  C12N15/8273

Abstract: This invention provides transgenic plant cells with recombinant DNA for expression of Arabidopsis thaliana heat stress transcription factor A-2, which is useful for imparting enhanced agronomic traits) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced trait.

Abstract translation: 本发明提供具有用于表达拟南芥热胁迫转录因子A-2的重组DNA的转基因植物细胞，其可用于赋予改良的农艺性状）转基因作物植物。 本发明还提供包含转基因植物细胞的转基因植物和后代种子，其中植物被选择用于具有增强的性状，所述性状选自由增强的水利用效率，增强的耐寒性，增加的产量，增强的氮利用效率，增强的氮利用效率， 种子蛋白和增强种子油。 还公开了用于制备具有增强特征的转基因种子和植物的方法。

公开(公告)号：US20190185877A1

公开(公告)日：2019-06-20

申请号：US16070733

申请日：2017-01-19

Applicant: Monsanto Technology LLC

Inventor： Patrick Boyle ,  David Caldwell ,  Jaishree Chittoor ,  Jintai Huang ,  Susanne Kjemtrup ,  Gerrit Segers

IPC: C12N15/82 ,  C07K14/415

CPC classification number: C12N15/8282 ,  C07K14/415

Abstract: The disclosure provides polynucleotide molecules encoding novel defensins conferring increased pest tolerance and/or pesticidal activity when expressed in a plant, and recombinant DNA constructs and vectors comprising these molecules. Methods of making transgenic plants comprising recombinant defensin-encoding polynucleotide molecules and constructs, and transgenic plants, plant parts and seeds produced by these methods are provided. Compositions comprising one or more novel defensins of the disclosure are also provided having pesticidal and/or anti-microbial activity, as well as methods of their use.

公开(公告)号：US11692182B2

公开(公告)日：2023-07-04

申请号：US15766662

申请日：2016-10-07

Applicant: Monsanto Technology LLC

Inventor： Jaishree Chittoor ,  Ervin Nagy ,  Keith H. Turner

IPC: C12N15/82 ,  C12N9/22 ,  C12N5/04 ,  C12N15/10 ,  C12N15/90 ,  C12N15/63 ,  C12N15/11

CPC classification number: C12N9/22 ,  C12N5/04 ,  C12N15/102 ,  C12N15/11 ,  C12N15/111 ,  C12N15/63 ,  C12N15/8213 ,  C12N15/90 ,  C12N15/902 ,  C07K2319/09 ,  C07K2319/60 ,  C12N2310/20 ,  C12N2510/00 ,  C12N2800/80

Abstract: Provided herein are systems, methods, and compositions for the modification of target DNA sequences. More particularly, systems, methods, and compositions for cleaving a target DNA in eukaryotic cells with a guide RNA capable of hybridizing with a target sequence and an RNA-guided DNA nuclease are provided. Also provided are vectors and vector systems which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods for identifying and validating novel CRISPR systems.