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15 条记录 (耗时 0.043 秒)

    Process for the purification of macrolides
    5.
    发明申请
    Process for the purification of macrolides 有权
    大环内酯类的纯化方法

    公开(公告)号：US20070173642A1

    公开(公告)日：2007-07-26

    申请号：US10596227

    申请日：2003-12-05

    申请人： Nitin Patil ,  Rakesh Mendhe ,  Anand Khedkar ,  Ramakrishnan Melarkode ,  Ramavana Gururaja

    发明人： Nitin Patil ,  Rakesh Mendhe ,  Anand Khedkar ,  Ramakrishnan Melarkode ,  Ramavana Gururaja

    IPC分类号： C07H17/08 ,  C07D491/14

    CPC分类号： C07D498/18

    摘要： Process for the recovery of a macrolide in substantially pure form comprising: a) treatment of an impure or crude macrolide with water immiscible solvent, b) optional concentration of the mixture, c) treatment with ammonia gas to phase out impurities, d) separation of impurities, d) optional concentration of the phase containing the macrolide, f) loading on silica gel chromatography, optionally reversed phase or pretreated with silver, and elution of the macrolide, g) affording the macrolide in substantially pure form, h) optional repetition of step f) and g) to afford the macrolide in substantially pure form. The macrolide is preferably tacrolimus, immunomycin or sirolimus.

    摘要翻译： 用于以基本上纯的形式回收大环内酯的方法，其包括：a）用与水不混溶的溶剂处理不纯或粗大环内酯，b）任选浓缩混合物，c）用氨气处理以逐步除去杂质，d） 杂质，d）含有大环内酯的相的任选浓度，f）在硅胶色谱法上任选反相或用银预处理，并用大环内酯洗脱，g）提供基本上纯的形式的大环内酯; h）任选的重复 步骤f）和g）以大致纯的形式提供大环内酯。 大环内酯优选为他克莫司，免疫霉素或西罗莫司。

    Process for the purification of macrolides
    8.
    发明授权
    Process for the purification of macrolides 有权
    大环内酯类的纯化方法

    公开(公告)号：US07473366B2

    公开(公告)日：2009-01-06

    申请号：US10596227

    申请日：2003-12-05

    申请人： Nitin Sopanrao Patil ,  Rakesh Mendhe ,  Anand Prakash Khedkar ,  Ramakrishnan Melarkode ,  Ramavana Gururaja

    发明人： Nitin Sopanrao Patil ,  Rakesh Mendhe ,  Anand Prakash Khedkar ,  Ramakrishnan Melarkode ,  Ramavana Gururaja

    IPC分类号： B01D15/08

    CPC分类号： C07D498/18

    摘要： The invention relates to a process for the recovery of a macrolide in substantial pure form including: a) treating an impure or crude macrolide with water immiscible solvent to form a mixture, b) optionally concentrating the mixture, c) treating with ammonia gas to phase out impurities, d) separating impurities, e) optionally concentrating a phase containing the macrolide, f) loading on silica gel chromatography, optionally reversed phase or pretreated with silver, and eluting the macrolide, g) affording the macrolide in the substantially pure form, h) optional repetition of steps f and g to afford the macrolide in the substantially pure form. The macrolide is preferably tacrolimus, immunomycin or sirolimus.

    摘要翻译： 本发明涉及以大体上纯的形式回收大环内酯的方法，包括：a）用不溶于水的溶剂处理不纯或粗大环内酯以形成混合物，b）任选地浓缩混合物，c）用氨气处理至相 除杂质，d）分离杂质，e）任选地浓缩含有大环内酯的相，f）在硅胶色谱法上任选反相或用银预处理，并用大环内酯洗脱，g）以大致纯的形式提供大环内酯， h）任选地重复步骤f和g以提供基本上纯的形式的大环内酯。 大环内酯优选为他克莫司，免疫霉素或西罗莫司。

    METHOD OF PRODUCING BIOLOGICALLY ACTIVE POLYPEPTIDE HAVING INSULINOTROPIC ACTIVITY
    10.
    发明申请
    METHOD OF PRODUCING BIOLOGICALLY ACTIVE POLYPEPTIDE HAVING INSULINOTROPIC ACTIVITY 有权
    生产具有胰岛素活性的生物活性多肽的方法

    公开(公告)号：US20100167342A1

    公开(公告)日：2010-07-01

    申请号：US12305460

    申请日：2007-06-20

    申请人： Ramakrishnan Melarkode ,  Akundi Venkata Sriram ,  Kedarnath Nanjund Sastry ,  Lakshmi Prabha Varadarajalu ,  Shrikumar Suryanarayan

    发明人： Ramakrishnan Melarkode ,  Akundi Venkata Sriram ,  Kedarnath Nanjund Sastry ,  Lakshmi Prabha Varadarajalu ,  Shrikumar Suryanarayan

    IPC分类号： C12P21/06

    CPC分类号： C07K14/46

    摘要： The present invention relates to a method of producing a biologically active polypeptide having insulinotropic activity, the method comprising steps of: (a) transforming a genetically modified host cell that has protease gene knockout, with a polynucleotide vector encoding the polypeptide; and (b) growing the transformed host cell to produce the biologically active polypeptide; and a method of producing a biologically active polypeptide having an N-terminal recognition site His-Gly with insulinotropic activity, the method comprising steps of: (a) transforming a genetically modified Pichia pastoris that has protease gene STE13 knockout, with a polynucleotide vector encoding the polypeptide; and (b) growing the transformed Pichia pastoris to produce the biologically active polypeptide.

    摘要翻译： 本发明涉及产生具有促胰岛素活性的生物活性多肽的方法，所述方法包括以下步骤：（a）用编码多肽的多核苷酸载体转化具有蛋白酶基因敲除的遗传修饰的宿主细胞; 和（b）使转化的宿主细胞生长以产生生物活性多肽; 以及产生具有促胰岛素活性的N-末端识别位点His-Gly的生物活性多肽的方法，所述方法包括以下步骤：（a）将具有蛋白酶基因STE13敲除的遗传修饰的巴斯德毕赤酵母转化为编码 多肽; 和（b）生长转化的巴斯德毕赤酵母以产生生物活性多肽。