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公开(公告)号：US07901918B2
公开(公告)日：2011-03-08
申请号：US12351454
申请日：2009-01-09
申请人： Mads Norregaard-Madsen , Peter Rahbek Ostergaard , Claus Bo Voge Christensen , Soren Flensted Lassen
发明人： Mads Norregaard-Madsen , Peter Rahbek Ostergaard , Claus Bo Voge Christensen , Soren Flensted Lassen
CPC分类号： C12N9/54 , A61K38/00 , C11D3/386 , Y02A50/473
摘要： The present invention relates to isolated proteases of the RP-II type and variants of RP-II proteases exhibiting improved properties in comparison to the parent RP-II protease, DNA constructs and vectors coding for the expression of said proteases and variants, host cells capable of expressing the proteases and variants from the DNA constructs, as well as a method of producing them by cultivating said host cells. The proteases may advantageously be used as constituents in detergent compositions and additives, optionally in combination with other enzymes such as proteases, lipases, cellulases, amylases, peroxidases or oxidases.
摘要翻译： 本发明涉及RP-II型分离的蛋白酶和与亲本RP-II蛋白酶相比表现出改进性质的RP-II蛋白酶的变体，DNA构建体和编码所述蛋白酶和变体的表达的载体，具有宿主细胞能力 从DNA构建体表达蛋白酶和变体，以及通过培养所述宿主细胞产生它们的方法。 蛋白酶可以有利地用作洗涤剂组合物和添加剂中的组分，任选地与其它酶如蛋白酶，脂肪酶，纤维素酶，淀粉酶，过氧化物酶或氧化酶组合使用。
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公开(公告)号：US20090148924A1
公开(公告)日：2009-06-11
申请号：US12351454
申请日：2009-01-09
申请人： Mads Norregaard-Madsen , Peter Rahbek Ostergaard , Claus Bo Voge Christensen , Soren Flensted Lassen
发明人： Mads Norregaard-Madsen , Peter Rahbek Ostergaard , Claus Bo Voge Christensen , Soren Flensted Lassen
IPC分类号： C12N9/50
CPC分类号： C12N9/54 , A61K38/00 , C11D3/386 , Y02A50/473
摘要： The present invention relates to isolated proteases of the RP-II type and variants of RP-II proteases exhibiting improved properties in comparison to the parent RP-II protease, DNA constructs and vectors coding for the expression of said proteases and variants, host cells capable of expressing the proteases and variants from the DNA constructs, as well as a method of producing them by cultivating said host cells. The proteases may advantageously be used as constituents in detergent compositions and additives, optionally in combination with other enzymes such as proteases, lipases, cellulases, amylases, peroxidases or oxidases.
摘要翻译： 本发明涉及RP-II型分离的蛋白酶和与亲本RP-II蛋白酶相比表现出改进性质的RP-II蛋白酶的变体，DNA构建体和编码所述蛋白酶和变体的表达的载体，具有宿主细胞能力 从DNA构建体表达蛋白酶和变体，以及通过培养所述宿主细胞产生它们的方法。 蛋白酶可以有利地用作洗涤剂组合物和添加剂中的组分，任选地与其它酶如蛋白酶，脂肪酶，纤维素酶，淀粉酶，过氧化物酶或氧化酶组合使用。
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公开(公告)号：US08119386B2
公开(公告)日：2012-02-21
申请号：US13014100
申请日：2011-01-26
申请人： Mads Norregaard-Madsen , Peter Rahbek Ostergaard , Claus Bo Voge Christensen , Soren Flensted Lassen
发明人： Mads Norregaard-Madsen , Peter Rahbek Ostergaard , Claus Bo Voge Christensen , Soren Flensted Lassen
CPC分类号： C12N9/54 , A61K38/00 , C11D3/386 , Y02A50/473
摘要： The present invention relates to isolated proteases of the RP-II type and variants of RP-II proteases exhibiting improved properties in comparison to the parent RP-II protease, DNA constructs and vectors coding for the expression of said proteases and variants, host cells capable of expressing the proteases and variants from the DNA constructs, as well as a method of producing them by cultivating said host cells. The proteases may advantageously be used as constituents in detergent compositions and additives, optionally in combination with other enzymes such as proteases, lipases, cellulases, amylases, peroxidases or oxidases.
摘要翻译： 本发明涉及RP-II型分离的蛋白酶和与亲本RP-II蛋白酶相比表现出改进性质的RP-II蛋白酶的变体，DNA构建体和编码所述蛋白酶和变体的表达的载体，具有宿主细胞能力 从DNA构建体表达蛋白酶和变体，以及通过培养所述宿主细胞产生它们的方法。 蛋白酶可以有利地用作洗涤剂组合物和添加剂中的组分，任选地与其它酶如蛋白酶，脂肪酶，纤维素酶，淀粉酶，过氧化物酶或氧化酶组合使用。
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公开(公告)号：US20110117624A1
公开(公告)日：2011-05-19
申请号：US13014100
申请日：2011-01-26
申请人： Mads Norregaard-Madsen , Peter Rabbek Østergaard , Claus Bo Voge Christensen , Søren Flensted Lassen
发明人： Mads Norregaard-Madsen , Peter Rabbek Østergaard , Claus Bo Voge Christensen , Søren Flensted Lassen
CPC分类号： C12N9/54 , A61K38/00 , C11D3/386 , Y02A50/473
摘要： The present invention relates to isolated proteases of the RP-II type and variants of RP-II proteases exhibiting improved properties in comparison to the parent RP-II protease, DNA constructs and vectors coding for the expression of said proteases and variants, host cells capable of expressing the proteases and variants from the DNA constructs, as well as a method of producing them by cultivating said host cells. The proteases may advantageously be used as constituents in detergent compositions and additives, optionally in combination with other enzymes such as proteases, lipases, cellulases, amylases, peroxidases or oxidases.
摘要翻译： 本发明涉及RP-II型分离的蛋白酶和与亲本RP-II蛋白酶相比表现出改进性质的RP-II蛋白酶的变体，DNA构建体和编码所述蛋白酶和变体的表达的载体，具有宿主细胞能力 从DNA构建体表达蛋白酶和变体，以及通过培养所述宿主细胞产生它们的方法。 蛋白酶可以有利地用作洗涤剂组合物和添加剂中的组分，任选地与其它酶如蛋白酶，脂肪酶，纤维素酶，淀粉酶，过氧化物酶或氧化酶组合使用。
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公开(公告)号：US07537922B2
公开(公告)日：2009-05-26
申请号：US11679371
申请日：2007-02-27
申请人： Mads Norregaard-Madsen , Peter Rahbek Østergaard , Claus Bo Voge Christensen , Søren Flensted Lassen
发明人： Mads Norregaard-Madsen , Peter Rahbek Østergaard , Claus Bo Voge Christensen , Søren Flensted Lassen
CPC分类号： C12N9/54 , A61K38/00 , C11D3/386 , Y02A50/473
摘要： The present invention relates to isolated proteases of the RP-II type and variants of RP-II proteases exhibiting improved properties in comparison to the parent RP-II protease, DNA constructs and vectors coding for the expression of said proteases and variants, host cells capable of expressing the proteases and variants from the DNA constructs, as well as a method of producing them by cultivating said host cells. The proteases may advantageously be used as constituents in detergent compositions and additives, optionally in combination with other enzymes such as proteases, lipases, cellulases, amylases, peroxidases or oxidases.
摘要翻译： 本发明涉及RP-II型分离的蛋白酶和与亲本RP-II蛋白酶相比表现出改进性质的RP-II蛋白酶的变体，DNA构建体和编码所述蛋白酶和变体的表达的载体，具有宿主细胞能力 从DNA构建体表达蛋白酶和变体，以及通过培养所述宿主细胞产生它们的方法。 蛋白酶可以有利地用作洗涤剂组合物和添加剂中的组分，任选地与其它酶如蛋白酶，脂肪酶，纤维素酶，淀粉酶，过氧化物酶或氧化酶组合使用。
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公开(公告)号：US09732310B2
公开(公告)日：2017-08-15
申请号：US12974519
申请日：2010-12-21
申请人： Henriette Draborg , Peter Kamp Hansen , Mads Eskelund Bjornvad , Mads Norregaard-Madsen , Mikael Mikkelsen
发明人： Henriette Draborg , Peter Kamp Hansen , Mads Eskelund Bjornvad , Mads Norregaard-Madsen , Mikael Mikkelsen
摘要： The present invention relates to novel subtilase variants exhibiting alterations relative to the parent subtilase in one or more properties including: Wash performance, thermal stability, storage stability or catalytic activity. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions.
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公开(公告)号：US20070224673A1
公开(公告)日：2007-09-27
申请号：US11679371
申请日：2007-02-27
IPC分类号： C12N9/64
CPC分类号： C12N9/54 , A61K38/00 , C11D3/386 , Y02A50/473
摘要： The present invention relates to isolated proteases of the RP-II type and variants of RP-II proteases exhibiting improved properties in comparison to the parent RP-II protease, DNA constructs and vectors coding for the expression of said proteases and variants, host cells capable of expressing the proteases and variants from the DNA constructs, as well as a method of producing them by cultivating said host cells. The proteases may advantageously be used as constituents in detergent compositions and additives, optionally in combination with other enzymes such as proteases, lipases, cellulases, amylases, peroxidases or oxidases.
摘要翻译： 本发明涉及RP-II型分离的蛋白酶和与亲本RP-II蛋白酶相比表现出改进性质的RP-II蛋白酶的变体，DNA构建体和编码所述蛋白酶和变体的表达的载体，具有宿主细胞能力 从DNA构建体表达蛋白酶和变体，以及通过培养所述宿主细胞产生它们的方法。 蛋白酶可以有利地用作洗涤剂组合物和添加剂中的组分，任选地与其它酶如蛋白酶，脂肪酶，纤维素酶，淀粉酶，过氧化物酶或氧化酶组合使用。
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公开(公告)号：US07888093B2
公开(公告)日：2011-02-15
申请号：US10699394
申请日：2003-10-31
申请人： Henriette Draborg , Peter Kamp Hansen , Mads Eskelund Bjornvad , Mads Norregaard-Madsen , Mikael Mikkelsen
发明人： Henriette Draborg , Peter Kamp Hansen , Mads Eskelund Bjornvad , Mads Norregaard-Madsen , Mikael Mikkelsen
摘要： The present invention relates to novel subtilase variants exhibiting alterations relative to the parent subtilase in one or more properties including: Wash performance, thermal stability, storage stability or catalytic activity. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions.
摘要翻译： 本发明涉及在一种或多种性质中显示相对于亲本脱羧酶的改变的新型脱羧酶变体，包括：洗涤性能，热稳定性，储存稳定性或催化活性。 本发明的变体适用于例如， 清洁剂或洗涤剂组合物，例如衣物洗涤剂组合物和洗碟组合物，包括自动洗碗组合物。
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公开(公告)号：US20090264300A1
公开(公告)日：2009-10-22
申请号：US12095778
申请日：2006-12-01
申请人： Thomas Franch , Mikkel Dybro Lundorf , Soren Nyboe Jacobsen , Eva Kampmann Olsen , Anne Lee Andersen , Anette Holtmann , Anders Holm Hansen , Anders Malling Sorensen , Anne Goldbech , Daen de Leon , Ditte Kievsmose Kaldor , Frank Abildgaard Slok , Gitte Nystrup Husemoen , Johannes Dolberg , Kim Birkebaek Jensen , Lene Pedersen , Mads Norregaard-Madsen , Michael Anders Godskesen , Sanne Schroder Glad , Sore Neve , Thomas Thisted , Tine Titilola Akinleminu Kronborg , Christian Klarner Sams , Jakob Felding , Per-Ola Freskgard , Alex Haahr Gouliaev , Henrik Pedersen
发明人： Thomas Franch , Mikkel Dybro Lundorf , Soren Nyboe Jacobsen , Eva Kampmann Olsen , Anne Lee Andersen , Anette Holtmann , Anders Holm Hansen , Anders Malling Sorensen , Anne Goldbech , Daen de Leon , Ditte Kievsmose Kaldor , Frank Abildgaard Slok , Gitte Nystrup Husemoen , Johannes Dolberg , Kim Birkebaek Jensen , Lene Pedersen , Mads Norregaard-Madsen , Michael Anders Godskesen , Sanne Schroder Glad , Sore Neve , Thomas Thisted , Tine Titilola Akinleminu Kronborg , Christian Klarner Sams , Jakob Felding , Per-Ola Freskgard , Alex Haahr Gouliaev , Henrik Pedersen
CPC分类号： C12N15/1068 , C12N15/1089 , C40B40/08 , C40B50/04 , C40B50/06
摘要： Disclosed is a method for obtaining a bifunctional complex comprising a molecule linked to a single stranded identifier oligonucleotide, wherein a nascent bifunctional complex comprising a chemical reaction site and a priming site for enzymatic addition of a tag is a) reacted at the chemical reaction site with one or more reactants, and b) reacted enzymatically at the priming site with one or more tag(s) identifying the reactant(s).
摘要翻译： 公开了一种获得包含与单链标识符寡核苷酸连接的分子的双功能复合物的方法，其中包含化学反应位点的新生双功能复合物和用于酶标记添加的引发位点是a）在化学反应位点与 一种或多种反应物，和b）在起始位点用一种或多种标记反应物的标签进行酶促反应。
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公开(公告)号：US20050054072A1
公开(公告)日：2005-03-10
申请号：US10639194
申请日：2003-08-11
摘要： The present invention relates to isolated proteases of the RP-II type and variants of RP-II proteases exhibiting improved properties in comparison to the parent RP-II protease, DNA constructs and vectors coding for the expression of said proteases and variants, host cells capable of expressing the proteases and variants from the DNA constructs, as well as a method of producing them by cultivating said host cells. The proteases may advantageously be used as constituents in detergent compositions and additives, optionally in combination with other enzymes such as proteases, lipases, cellulases, amylases, peroxidases or oxidases.
摘要翻译： 本发明涉及RP-II型分离的蛋白酶和与亲本RP-II蛋白酶相比表现出改进性质的RP-II蛋白酶的变体，DNA构建体和编码所述蛋白酶和变体的表达的载体，具有宿主细胞能力 从DNA构建体表达蛋白酶和变体，以及通过培养所述宿主细胞产生它们的方法。 蛋白酶可以有利地用作洗涤剂组合物和添加剂中的组分，任选地与其它酶如蛋白酶，脂肪酶，纤维素酶，淀粉酶，过氧化物酶或氧化酶组合使用。
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