公开(公告)号：US5135863A

公开(公告)日：1992-08-04

申请号：US290487

申请日：1988-12-23

申请人： Mae Wan Leng Hu ,  Cheng-I Lin ,  Chen-jung Hsu ,  James V. Freeman ,  Marcel Pirio ,  Edwin F. Ullman

发明人： Mae Wan Leng Hu ,  Cheng-I Lin ,  Chen-jung Hsu ,  James V. Freeman ,  Marcel Pirio ,  Edwin F. Ullman

IPC分类号： G01N33/53 ,  C07C233/18 ,  C07C323/12 ,  C07C323/29 ,  C07C323/32 ,  C07C323/41 ,  C07C323/60 ,  G01N33/535 ,  G01N33/577 ,  G01N33/94

CPC分类号： G01N33/946 ,  C07C233/18 ,  C07C323/12 ,  C07C323/32 ,  C07C323/41 ,  G01N33/535 ,  Y10S435/964 ,  Y10S436/816 ,  Y10S436/822

摘要： The instant invention is directed toward an immunoassay which can determine the presence of amphetamines in a sample suspected of containing amphetamine and/or methamphetamine by employing at least two conjugates, each comprised of a functionally similar label bound to an amphetamine analog and a methamphetamine analog respectively and an antibody to amphetamine and an antibody to methamphetamine wherein at least one of the antibodies is a monoclonal antibody.

公开(公告)号：US5328828A

公开(公告)日：1994-07-12

申请号：US916568

申请日：1992-07-20

申请人： Mae W. Hu ,  Cheng-I Lin ,  Chen-jung Hsu ,  James V. Freeman ,  Marcel Pirio ,  Edwin F. Ullman

发明人： Mae W. Hu ,  Cheng-I Lin ,  Chen-jung Hsu ,  James V. Freeman ,  Marcel Pirio ,  Edwin F. Ullman

IPC分类号： G01N33/53 ,  C07C233/18 ,  C07C323/12 ,  C07C323/29 ,  C07C323/32 ,  C07C323/41 ,  C07C323/60 ,  G01N33/535 ,  G01N33/577 ,  G01N33/94 ,  G01N33/536 ,  G01N33/537 ,  G01N33/542

CPC分类号： G01N33/946 ,  C07C233/18 ,  C07C323/12 ,  C07C323/32 ,  C07C323/41 ,  G01N33/535 ,  Y10S435/964 ,  Y10S436/816 ,  Y10S436/822

摘要： The instant invention is directed toward an immunoassay which can determine the presence of amphetamines in a sample suspected of containing amphetamine and/or methamphetamine by employing at least two conjugates, each comprised of a functionally similar label bound to an amphetamine analog and a methamphetamine analog respectively and an antibody to amphetamine and an antibody to methamphetamine wherein at least one of the antibodies is a monoclonal antibody.

摘要翻译： 本发明涉及免疫测定，其可以通过使用至少两种共轭物来确定怀疑含有苯丙胺和/或甲基苯丙胺的样品中苯丙胺的存在，每个缀合物分别与分别与苯丙胺类似物和甲基苯丙胺类似物结合的功能相似的标记 以及针对苯丙胺的抗体和针对甲基苯丙胺的抗体，其中至少一种抗体是单克隆抗体。

公开(公告)号：US07033775B2

公开(公告)日：2006-04-25

申请号：US10888033

申请日：2004-07-09

申请人： Edwin F. Ullman ,  Marcel Pirio ,  Mary C. Ericson ,  Daniel B. Wagner ,  Dariush Davalian

发明人： Edwin F. Ullman ,  Marcel Pirio ,  Mary C. Ericson ,  Daniel B. Wagner ,  Dariush Davalian

IPC分类号： G01N33/53

CPC分类号： G01N33/946 ,  G01N33/54386 ,  Y10S435/975 ,  Y10S436/805 ,  Y10S436/811 ,  Y10S436/815 ,  Y10S436/816 ,  Y10S436/817 ,  Y10S436/901

摘要： Methods, compositions and kits are disclosed. The methods are directed to determining the presence of one or more analytes in a sample suspected of containing any one of a plurality of the analytes. A combination is provided comprising in a medium (i) the sample, (ii) a binding partner for each of the analytes, (iii) for each of the analytes, a first reagent comprising a member of a signal producing system, a ligand and an analyte analog, and (iv) a second reagent comprising a binding partner for the ligand. The binding of the binding partner for the ligand is affected by the presence of an analyte and alters the amount of signal produced by the member of the signal producing system. The signal thus is modulated if one or more of the analytes are present in the sample. The amount of the signal is determined and is related to the presence of one or more of the analytes in the sample. The method may be homogeneous or heterogeneous.

公开(公告)号：US07101682B2

公开(公告)日：2006-09-05

申请号：US11268323

申请日：2005-11-07

申请人： Edwin F. Ullman ,  Marcel Pirio ,  Mary C. Ericson ,  Daniel B. Wagner ,  Dariush Davalian

发明人： Edwin F. Ullman ,  Marcel Pirio ,  Mary C. Ericson ,  Daniel B. Wagner ,  Dariush Davalian

IPC分类号： G01N33/53

CPC分类号： G01N33/946 ,  G01N33/54386 ,  Y10S435/975 ,  Y10S436/805 ,  Y10S436/811 ,  Y10S436/815 ,  Y10S436/816 ,  Y10S436/817 ,  Y10S436/901

摘要： Methods, compositions and kits are disclosed. The methods are directed to determining the presence of one or more analytes in a sample suspected of containing any one of a plurality of the analytes. A combination is provided comprising in a medium (i) the sample, (ii) a binding partner for each of the analytes, (iii) for each of the analytes, a first reagent comprising a member of a signal producing system, a ligand and an analyte analog, and (iv) a second reagent comprising a binding partner for the ligand. The binding of the binding partner for the ligand is affected by the presence of an analyte and alters the amount of signal produced by the member of the signal producing system. The signal thus is modulated if one or more of the analytes are present in the sample. The amount of the signal is determined and is related to the presence of one or more of the analytes in the sample. The method may be homogeneous or heterogeneous.

摘要翻译： 公开了方法，组合物和试剂盒。 该方法旨在确定怀疑含有多种分析物中的任何一种的样品中一种或多种分析物的存在。 提供的组合包括在介质（i）样品中，（ii）每种分析物的结合配偶体，（iii）对于每种分析物，包含信号产生系统的成员的第一试剂，配体和 分析物类似物，和（iv）包含配体的结合配偶体的第二试剂。 配体的结合配偶体的结合受到分析物的存在的影响，并改变由信号产生系统的成员产生的信号量。 如果一个或多个分析物存在于样品中，则信号因此被调制。 确定信号的量，并与样品中一种或多种分析物的存在相关。 该方法可以是均匀的或不均匀的。

公开(公告)号：US06797481B1

公开(公告)日：2004-09-28

申请号：US09691383

申请日：2000-10-17

申请人： Edwin F. Ullman ,  Marcel Pirio ,  Mary C. Ericson ,  Daniel B. Wagner ,  Dariush Davalian

发明人： Edwin F. Ullman ,  Marcel Pirio ,  Mary C. Ericson ,  Daniel B. Wagner ,  Dariush Davalian

IPC分类号： G01N3353

CPC分类号： G01N33/946 ,  G01N33/54386 ,  Y10S435/975 ,  Y10S436/805 ,  Y10S436/811 ,  Y10S436/815 ,  Y10S436/816 ,  Y10S436/817 ,  Y10S436/901

摘要： Methods, compositions and kits are disclosed. The methods are directed to determining the presence of one or more analytes in a sample suspected of containing any one of a plurality of the analytes. A combination is provided comprising in a medium (i) the sample, (ii) a binding partner for each of the analytes, (iii) for each of the analytes, a first reagent comprising a member of a signal producing system, a ligand and an analyte analog, and (iv) a second reagent comprising a binding partner for the ligand. The binding of the binding partner for the ligand is affected by the presence of an analyte and alters the amount of signal produced by the member of the signal producing system. The signal thus is modulated if one or more of the analytes are present in the sample. The amount of the signal is determined and is related to the presence of one or more of the analytes in the sample. The method may be homogeneous or heterogeneous.

摘要翻译： 公开了方法，组合物和试剂盒。 该方法旨在确定怀疑含有多种分析物中的任何一种的样品中一种或多种分析物的存在。 提供的组合包括在介质（i）样品中，（ii）每种分析物的结合配偶体，（iii）对于每种分析物，包含信号产生系统的成员的第一试剂，配体和 分析物类似物，和（iv）包含配体的结合配偶体的第二试剂。 配体的结合配偶体的结合受到分析物的存在的影响，并改变由信号产生系统的成员产生的信号量。 如果一个或多个分析物存在于样品中，则信号因此被调制。 确定信号的量，并与样品中一种或多种分析物的存在相关。 该方法可以是均匀的或不均匀的。

公开(公告)号：US5840508A

公开(公告)日：1998-11-24

申请号：US455424

申请日：1995-05-31

申请人： Arthur C. Switchenko ,  Nurith Kurn ,  Christian Neukom ,  Marcel Pirio ,  Donald E. Berger, Jr. ,  Edwin F. Ullman

发明人： Arthur C. Switchenko ,  Nurith Kurn ,  Christian Neukom ,  Marcel Pirio ,  Donald E. Berger, Jr. ,  Edwin F. Ullman

IPC分类号： G01N33/531 ,  A61K39/00 ,  B01F17/00 ,  C07C323/12 ,  C07C323/14 ,  C07K1/32 ,  C07K14/22 ,  C07K14/295 ,  G01N33/569 ,  G01N33/68 ,  G01N33/92

CPC分类号： B01F17/0064 ,  B01F17/0021 ,  B01F17/0057 ,  C07C323/14 ,  C07K1/145 ,  C07K1/32 ,  C07K14/22 ,  C07K14/295 ,  G01N33/56927 ,  G01N33/6803 ,  G01N33/92 ,  Y10S435/961 ,  Y10S435/962 ,  Y10S435/967 ,  Y10S436/825 ,  Y10T436/10 ,  Y10T436/107497 ,  Y10T436/25 ,  Y10T436/25125

摘要： Compounds having detergent properties are disclosed. When a modifying reagent is brought into contact with these compounds, the detergent properties are decreased. These compounds are useful, for example, as solubilizing agents for microbial antigens and/or antibodies and for reversibly wetting hydrophobic surfaces. Accordingly, methods are disclosed for increasing the hydrophilic properties of a material, such as a microbial antigen and/or antibody, the methods generally comprising the steps of contacting the material with the compound having detergent properties and a modifiable group, and modifying the compound with a modifying reagent. Kits are also disclosed for use in accordance with this methodology.

公开(公告)号：US5670690A

公开(公告)日：1997-09-23

申请号：US455920

申请日：1995-05-31

申请人： Arthur C. Switchenko ,  Nurith Kurn ,  Christian Neukom ,  Marcel Pirio ,  Donald E. Berger, Jr. ,  Edwin F. Ullman

发明人： Arthur C. Switchenko ,  Nurith Kurn ,  Christian Neukom ,  Marcel Pirio ,  Donald E. Berger, Jr. ,  Edwin F. Ullman

IPC分类号： G01N33/531 ,  A61K39/00 ,  B01F17/00 ,  C07C323/12 ,  C07C323/14 ,  C07K1/32 ,  C07K14/22 ,  C07K14/295 ,  G01N33/569 ,  G01N33/68 ,  G01N33/92 ,  C07C305/04

CPC分类号： B01F17/0064 ,  B01F17/0021 ,  B01F17/0057 ,  C07C323/14 ,  C07K1/145 ,  C07K1/32 ,  C07K14/22 ,  C07K14/295 ,  G01N33/56927 ,  G01N33/6803 ,  G01N33/92 ,  Y10S435/961 ,  Y10S435/962 ,  Y10S435/967 ,  Y10S436/825 ,  Y10T436/10 ,  Y10T436/107497 ,  Y10T436/25 ,  Y10T436/25125

摘要： Compounds having detergent properties are disclosed. When a modifying reagent is brought into contact with these compounds, the detergent properties are decreased. These compounds are useful, for example, as solubilizing agents for microbial antigens and/or antibodies and for reversibly wetting hydrophobic surfaces. Accordingly, methods are disclosed for increasing the hydrophilic properties of a material, such as a microbial antigen and/or antibody, the methods generally comprising the steps of contacting the material with the compound having detergent properties and a modifiable group, and modifying the compound with a modifying reagent. Kits are also disclosed for use in accordance with this methodology.

摘要翻译： 公开了具有洗涤剂性质的化合物。 当改性试剂与这些化合物接触时，洗涤剂性能降低。 这些化合物可用作例如微生物抗原和/或抗体的增溶剂，并可逆地润湿疏水性表面。 因此，公开了增加材料如微生物抗原和/或抗体的亲水性质的方法，通常包括使材料与具有洗涤剂性质和可修饰基团的化合物接触的步骤，并将化合物与 修饰试剂。 套件也按照这种方法被公开使用。

公开(公告)号：US5563038A

公开(公告)日：1996-10-08

申请号：US154340

申请日：1993-11-18

申请人： Arthur C. Switchenko ,  Nurith Kurn ,  Christian Neukom ,  Marcel Pirio ,  Donald E. Berger, Jr. ,  Edwin F. Ullman

发明人： Arthur C. Switchenko ,  Nurith Kurn ,  Christian Neukom ,  Marcel Pirio ,  Donald E. Berger, Jr. ,  Edwin F. Ullman

IPC分类号： G01N33/531 ,  A61K39/00 ,  B01F17/00 ,  C07C323/12 ,  C07C323/14 ,  C07K1/32 ,  C07K14/22 ,  C07K14/295 ,  G01N33/569 ,  G01N33/68 ,  G01N33/92

CPC分类号： B01F17/0064 ,  B01F17/0021 ,  B01F17/0057 ,  C07C323/14 ,  C07K1/145 ,  C07K1/32 ,  C07K14/22 ,  C07K14/295 ,  G01N33/56927 ,  G01N33/6803 ,  G01N33/92 ,  Y10S435/961 ,  Y10S435/962 ,  Y10S435/967 ,  Y10S436/825 ,  Y10T436/10 ,  Y10T436/107497 ,  Y10T436/25 ,  Y10T436/25125

摘要： Compounds having detergent properties are disclosed. When a modifying reagent is brought into contact with these compounds, the detergent properties are decreased. These compounds are useful, for example, as solubilizing agents for microbial antigens and/or antibodies and for reversibly wetting hydrophobic surfaces. Accordingly, methods are disclosed for increasing the hydrophilic properties of a material, such as a microbial antigen and/or antibody, the methods generally comprising the steps of contacting the material with the compound having detergent properties and a modifiable group, and modifying the compound with a modifying reagent. Kits are also disclosed for use in accordance with this methodology.

摘要翻译： 公开了具有洗涤剂性质的化合物。 当改性试剂与这些化合物接触时，洗涤剂性能降低。 这些化合物可用作例如微生物抗原和/或抗体的增溶剂，并可逆地润湿疏水性表面。 因此，公开了增加材料如微生物抗原和/或抗体的亲水性质的方法，通常包括使材料与具有洗涤剂性质和可修饰基团的化合物接触的步骤，并将化合物与 修饰试剂。 套件也按照这种方法被公开使用。

公开(公告)号：US5116726A

公开(公告)日：1992-05-26

申请号：US223501

申请日：1988-07-25

申请人： Arthur C. Switchenko ,  Nurith Kurn ,  Christian Neukom ,  Marcel Pirio ,  Donald E. Berger, Jr. ,  Edwin F. Ullman

发明人： Arthur C. Switchenko ,  Nurith Kurn ,  Christian Neukom ,  Marcel Pirio ,  Donald E. Berger, Jr. ,  Edwin F. Ullman

IPC分类号： G01N33/531 ,  A61K39/00 ,  B01F17/00 ,  C07C323/12 ,  C07C323/14 ,  C07K1/32 ,  C07K14/22 ,  C07K14/295 ,  G01N33/569 ,  G01N33/68 ,  G01N33/92

CPC分类号： B01F17/0064 ,  B01F17/0021 ,  B01F17/0057 ,  C07C323/14 ,  C07K1/145 ,  C07K1/32 ,  C07K14/22 ,  C07K14/295 ,  G01N33/56927 ,  G01N33/6803 ,  G01N33/92 ,  Y10S435/961 ,  Y10S435/962 ,  Y10S435/967 ,  Y10S436/825 ,  Y10T436/10 ,  Y10T436/107497 ,  Y10T436/25 ,  Y10T436/25125

摘要： Compounds having detergent properties are disclosed. When a modifying reagent is brought into contact with these compounds, the detergent properties are decreased. These compounds are useful, for example, as solubilizing agents for microbial antigens and/or antibodies and for reversibly wetting hydrophobic surfaces. Accordingly, methods are disclosed for increasing the hydrophilic properties of a material, such as a microbial antigen and/or antibody, the methods generally comprising the steps of contacting the material with the compound having detergent properties and a modifiable group, and modifying the compound with a modifying reagent. Kits are also disclosed for use in accordance with this methodology.

摘要翻译： 公开了具有洗涤剂性质的化合物。 当改性试剂与这些化合物接触时，洗涤剂性能降低。 这些化合物可用作例如微生物抗原和/或抗体的增溶剂，并可逆地润湿疏水性表面。 因此，公开了增加材料如微生物抗原和/或抗体的亲水性质的方法，通常包括使材料与具有洗涤剂性质和可修饰基团的化合物接触的步骤，并将化合物与 修饰试剂。 套件也按照这种方法被公开使用。

公开(公告)号：US5207985A

公开(公告)日：1993-05-04

申请号：US718067

申请日：1991-06-20

申请人： Cheng-I Lin ,  Marcel Pirio

发明人： Cheng-I Lin ,  Marcel Pirio

IPC分类号： C07D273/00 ,  G01N31/22 ,  G01N33/84

CPC分类号： C07D273/00 ,  G01N31/22 ,  G01N33/84

摘要： Novel compounds and assay methods are provided for determining the presence of lithium in serum, plasma, urine or other sample without deproteinization. The novel compounds are water soluble derivatives of TMC-crownformazans and provide signal enhancement by increased absorbance of the dye-lithium complex over the dye anion.

摘要翻译： 提供了新的化合物和测定方法，用于确定血清，血浆，尿液或其他样品中锂的存在，而无需脱蛋白。 这些新型化合物是TMC-冠状表面活性剂的水溶性衍生物，并通过增加染料 - 锂络合物在染料阴离子上的吸光度而提供信号增强。