公开(公告)号：US20070280857A1

公开(公告)日：2007-12-06

申请号：US11422058

申请日：2006-06-02

申请人： Maengseok Song ,  Joon Mo Yang ,  Julie C. Lee ,  Nigel P. Beard ,  Yuh-Min Chiang ,  Roy H. Tan ,  Carol Schembri

发明人： Maengseok Song ,  Joon Mo Yang ,  Julie C. Lee ,  Nigel P. Beard ,  Yuh-Min Chiang ,  Roy H. Tan ,  Carol Schembri

IPC分类号： B01L3/02

CPC分类号： B01L3/502723 ,  B01L3/502746 ,  B01L3/502784 ,  B01L2200/0642 ,  B01L2200/0673 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0487 ,  B01L2400/086 ,  C12Q1/6806 ,  Y10T436/2575

摘要： A microfluidic device may include a sample distribution network including a plurality of sample chambers configured to be loaded with biological sample for biological testing of the biological sample while in the sample chambers, the biological sample having a meniscus that moves within the sample chambers during loading. The sample distribution network may further include a plurality of inlet channels, each inlet channel being in flow communication with and configured to flow biological sample to a respective sample chamber, and a plurality of outlet channels, each outlet channel being in flow communication and configured to flow biological sample from a respective sample chamber. At least some of the sample chambers may include a physical modification configured to control the movement of the meniscus so as to control bubble formation within the at least some sample chambers. At least some of the sample chambers may include a dried reagent positioned within the at least some sample chambers proximate the inlet channels in flow communication with the at least some sample chambers.

摘要翻译： 微流体装置可以包括样品分配网络，其包括多个样品室，其被配置为装载用于生物样品的生物样品的生物样品，而在样品室中，生物样品具有在加载期间在样品室内移动的弯液面。 样品分配网络还可以包括多个入口通道，每个入口通道与生物样品流动连通并配置成将生物样品流动到相应的样品室，以及多个出口通道，每个出口通道流动连通并且被配置为 从相应的样品室流动生物样品。 至少一些样品室可以包括物理修改，其被配置为控制弯液面的运动，以便控制至少一些样品室内的气泡形成。 至少一些样品室可以包括位于与至少一些样品室流动连通的入口通道附近的至少一些样品室内的干燥试剂。

公开(公告)号：US20070280856A1

公开(公告)日：2007-12-06

申请号：US11422057

申请日：2006-06-02

申请人： Umberto Ulmanella ,  Eric S. Nordman ,  Maengseok Song ,  Joon Mo Yang ,  Julie C. Lee ,  Nigel P. Beard ,  Min Yue ,  Carol Schembri ,  David Liu

发明人： Umberto Ulmanella ,  Eric S. Nordman ,  Maengseok Song ,  Joon Mo Yang ,  Julie C. Lee ,  Nigel P. Beard ,  Min Yue ,  Carol Schembri ,  David Liu

IPC分类号： B01L3/02

CPC分类号： B01L3/502746 ,  B01L3/502723 ,  B01L3/502784 ,  B01L2200/0642 ,  B01L2200/0673 ,  B01L2200/0684 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/0487 ,  B01L2400/086

摘要： A microfluidic device may include a sample distribution network including a plurality of sample chambers configured to be loaded with biological sample for biological testing of the biological sample while in the sample chambers, the biological sample having a meniscus that moves within the sample chambers during loading. The sample distribution network may further include a plurality of inlet channels, each inlet channel being in flow communication with and configured to flow biological sample to a respective sample chamber, and a plurality of outlet channels, each outlet channel being in flow communication and configured to flow biological sample from a respective sample chamber. At least some of the sample chambers may include a physical modification configured to control the movement of the meniscus so as to control bubble formation within the at least some sample chambers. At least some of the sample chambers may include a dried reagent positioned within the at least some sample chambers proximate the inlet channels in flow communication with the at least some sample chambers.

摘要翻译： 微流体装置可以包括样品分配网络，其包括多个样品室，其被配置为装载用于生物样品的生物样品的生物样品，而在样品室中，生物样品具有在加载期间在样品室内移动的弯液面。 样品分配网络还可以包括多个入口通道，每个入口通道与生物样品流动连通并配置成将生物样品流动到相应的样品室，以及多个出口通道，每个出口通道流动连通并且被配置为 从相应的样品室流动生物样品。 至少一些样品室可以包括物理修改，其被配置为控制弯液面的运动，以便控制至少一些样品室内的气泡形成。 至少一些样品室可以包括位于与至少一些样品室流动连通的入口通道附近的至少一些样品室内的干燥试剂。

公开(公告)号：US08530158B2

公开(公告)日：2013-09-10

申请号：US13104304

申请日：2011-05-10

申请人： Maengseok Song ,  Achim Karger ,  Patrick Kinney

发明人： Maengseok Song ,  Achim Karger ,  Patrick Kinney

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12M1/34 ,  C12M3/00

CPC分类号： B01L3/502761 ,  B01F5/0652 ,  B01F2003/0823 ,  B01L7/52 ,  B01L2200/0673 ,  B01L2400/0439 ,  C12Q1/6806

摘要： Systems and methods for processing a biological sample are provided herein. For example, the system can be configured to deaggregate/declump a sample before, during, and/or after sample preparation and/or sample analysis. For example, the system can include a deaggregation device/system in communication with, for example, a nucleic acid amplification process (e.g., an ePCR system). Various embodiments of the deaggregation device are provided herein. For example, in some embodiments, the deaggregation device can include a valve, a valve manifold, a conduit, a channel, or some combinations thereof.

摘要翻译： 本文提供了用于处理生物样品的系统和方法。 例如，可以将系统配置为在样品制备和/或样品分析之前，期间和/或之后对样品进行解聚/递归。 例如，该系统可以包括与例如核酸扩增过程（例如，ePCR系统）通信的解聚集装置/系统。 本文提供了解聚集装置的各种实施例。 例如，在一些实施例中，解聚装置可以包括阀，阀歧管，导管，通道或其一些组合。