公开(公告)号：US08154796B2

公开(公告)日：2012-04-10

申请号：US11593842

申请日：2006-11-07

申请人： Makoto Araki ,  Hiroshi Sasaki ,  Tatsuo Nakata ,  Makio Ueno

发明人： Makoto Araki ,  Hiroshi Sasaki ,  Tatsuo Nakata ,  Makio Ueno

IPC分类号： G02B21/06

CPC分类号： G02B21/0032 ,  G01N21/6458 ,  G01N2021/6439 ,  G02B21/0044 ,  G02B21/0076 ,  G02B21/0084 ,  G02B21/16 ,  G02B21/32

摘要： A microscope apparatus that can observe into the interior of a specimen and that can apply an optical stimulus over a wide area within a short period of time is provided. The microscope apparatus comprises at least one observation scanning optical system including a laser light source for emitting observation laser light, an objective lens, and a scanning optical system for two-dimensionally scanning the observation laser light in a predetermined examination plane of the specimen via the objective lens; and at least one stimulus optical system which includes a lamp light source for emitting light having a wavelength used for optical stimulation and which irradiates the specimen with the light emitted from the lamp light source.

摘要翻译： 提供了可以观察到样本内部并且可以在短时间内在广泛区域上施加光学刺激的显微镜装置。 显微镜装置包括至少一个观察扫描光学系统，其包括用于发射观察激光的激光光源，物镜和用于在样本的预定检查平面中二维扫描观察激光的扫描光学系统 物镜; 以及至少一个刺激光学系统，其包括用于发射具有用于光学刺激的波长的光的灯光源，并且从灯光源发射的光照射样本。

公开(公告)号：US20070109634A1

公开(公告)日：2007-05-17

申请号：US11593842

申请日：2006-11-07

申请人： Makoto Araki ,  Hiroshi Sasaki ,  Tatsuo Nakata ,  Makio Ueno

发明人： Makoto Araki ,  Hiroshi Sasaki ,  Tatsuo Nakata ,  Makio Ueno

IPC分类号： G02B21/06

CPC分类号： G02B21/0032 ,  G01N21/6458 ,  G01N2021/6439 ,  G02B21/0044 ,  G02B21/0076 ,  G02B21/0084 ,  G02B21/16 ,  G02B21/32

摘要： A microscope apparatus that can observe into the interior of a specimen and that can apply an optical stimulus over a wide area within a short period of time is provided. The microscope apparatus comprises at least one observation scanning optical system including a laser light source for emitting observation laser light, an objective lens, and a scanning optical system for two-dimensionally scanning the observation laser light in a predetermined examination plane of the specimen via the objective lens; and at least one stimulus optical system which includes a lamp light source for emitting light having a wavelength used for optical stimulation and which irradiates the specimen with the light emitted from the lamp light source.

摘要翻译： 提供了可以观察到样本内部并且可以在短时间内在广泛区域上施加光学刺激的显微镜装置。 显微镜装置包括至少一个观察扫描光学系统，其包括用于发射观察激光的激光光源，物镜和用于在样本的预定检查平面中二维扫描观察激光的扫描光学系统 物镜; 以及至少一个刺激光学系统，其包括用于发射具有用于光学刺激的波长的光的灯光源，并且从灯光源发射的光照射样本。

公开(公告)号：US07889341B2

公开(公告)日：2011-02-15

申请号：US12148671

申请日：2008-04-21

申请人： Makoto Araki ,  Yasunari Matsukawa ,  Hiroshi Sasaki ,  Makio Ueno

发明人： Makoto Araki ,  Yasunari Matsukawa ,  Hiroshi Sasaki ,  Makio Ueno

IPC分类号： G01N21/25

CPC分类号： G02B21/0076 ,  G01N21/6458 ,  G02B21/0064 ,  G02B21/008

摘要： A multiphoton-excitation laser scanning microscope capable of efficiently collecting fluorescence emitted from a specimen to acquire a brighter multiphoton-excitation fluorescence image is provided. This multiphoton-excitation laser scanning microscope includes a multiphoton-excitation laser light source for emitting ultrashort pulsed laser light, a light-scanning unit configured to scan a specimen with the ultrashort pulsed laser light emitted from the multiphoton-excitation laser light source in two dimensions, an objective lens configured to focus the ultrashort pulsed laser light scanned by the light-scanning unit on the specimen, a collector lens disposed opposite the objective lens, with the light-scanning unit disposed therebetween, to collect fluorescence emitted from the specimen, and a light detector configured to detect the fluorescence collected by the collector lens. The collector lens has a higher numerical aperture and a larger field number than the objective lens.

摘要翻译： 提供能够有效地收集从样本发出的荧光以获得更明亮的多光子激发荧光图像的多光子激发激光扫描显微镜。 该多光子激发激光扫描显微镜包括用于发射超短脉冲激光的多光子激发激光光源，被配置为使用从多光子激发激光光源发射的超短脉冲激光二维扫描样本的光扫描单元 配置为将由所述光扫描单元扫描的超短脉冲激光对准在所述检体上的物镜，与所述物镜相对设置的集光透镜，其间设置有所述光扫描单元，以收集从所述检体发出的荧光，以及 光检测器，被配置为检测由所述收集透镜收集的荧光。 收集透镜具有比物镜更高的数值孔径和更大的场数。

公开(公告)号：US20080266551A1

公开(公告)日：2008-10-30

申请号：US12148671

申请日：2008-04-21

申请人： Makoto Araki ,  Yasunari Matsukawa ,  Hiroshi Sasaki ,  Makio Ueno

发明人： Makoto Araki ,  Yasunari Matsukawa ,  Hiroshi Sasaki ,  Makio Ueno

IPC分类号： G01N21/00 ,  G01J3/30

CPC分类号： G02B21/0076 ,  G01N21/6458 ,  G02B21/0064 ,  G02B21/008

摘要： A multiphoton-excitation laser scanning microscope capable of efficiently collecting fluorescence emitted from a specimen to acquire a brighter multiphoton-excitation fluorescence image is provided. This multiphoton-excitation laser scanning microscope includes a multiphoton-excitation laser light source for emitting ultrashort pulsed laser light, a light-scanning unit configured to scan a specimen with the ultrashort pulsed laser light emitted from the multiphoton-excitation laser light source in two dimensions, an objective lens configured to focus the ultrashort pulsed laser light scanned by the light-scanning unit on the specimen, a collector lens disposed opposite the objective lens, with the light-scanning unit disposed therebetween, to collect fluorescence emitted from the specimen, and a light detector configured to detect the fluorescence collected by the collector lens. The collector lens has a higher numerical aperture and a larger field number than the objective lens.

摘要翻译： 提供能够有效地收集从样本发出的荧光以获得更明亮的多光子激发荧光图像的多光子激发激光扫描显微镜。 该多光子激发激光扫描显微镜包括用于发射超短脉冲激光的多光子激发激光光源，被配置为使用从多光子激发激光光源发射的超短脉冲激光二维扫描样本的光扫描单元 配置为将由所述光扫描单元扫描的超短脉冲激光对准在所述检体上的物镜，与所述物镜相对设置的集光透镜，其间设置有所述光扫描单元，以收集从所述检体发出的荧光，以及 光检测器，被配置为检测由所述收集透镜收集的荧光。 收集透镜具有比物镜更高的数值孔径和更大的场数。

公开(公告)号：US20080151366A1

公开(公告)日：2008-06-26

申请号：US12002508

申请日：2007-12-17

申请人： Akinori Araya ,  Tatsuo Nakata ,  Makio Ueno

发明人： Akinori Araya ,  Tatsuo Nakata ,  Makio Ueno

IPC分类号： G02B21/00

CPC分类号： G02B21/0036

摘要： It is possible to achieve a required field number and numerical aperture for microscope observation at a scanning speed equal to video-rate or higher and also to change the scanning speed with a simple configuration. The invention provides a laser microscope including a laser light source; a scanning unit configured to scan a specimen with laser light emitted from the laser light source; and an objective lens configured to focus the laser light scanned by the scanning unit on the specimen. The scanning unit is provided with an electro-optical deflecting element including an electro-optical crystal in which a refractive index gradient is induced by injecting electric current.

摘要翻译： 可以以等于视频速率或更高的扫描速度实现用于显微镜观察的所需场数和数值孔径，并且还可以以简单的配置改变扫描速度。 本发明提供一种包括激光光源的激光显微镜; 扫描单元，被配置为用从所述激光光源发射的激光扫描样本; 以及配置为将由扫描单元扫描的激光聚焦在样本上的物镜。 扫描单元设置有电光偏转元件，其包括通过注入电流而引起折射率梯度的电光晶体。

公开(公告)号：US07969653B2

公开(公告)日：2011-06-28

申请号：US12002508

申请日：2007-12-17

申请人： Akinori Araya ,  Tatsuo Nakata ,  Makio Ueno

发明人： Akinori Araya ,  Tatsuo Nakata ,  Makio Ueno

IPC分类号： G02B21/06 ,  G02B21/00

CPC分类号： G02B21/0036

摘要： It is possible to achieve a required field number and numerical aperture for microscope observation at a scanning speed equal to video-rate or higher and also to change the scanning speed with a simple configuration. The invention provides a laser microscope including a laser light source; a scanning unit configured to scan a specimen with laser light emitted from the laser light source; and an objective lens configured to focus the laser light scanned by the scanning unit on the specimen. The scanning unit is provided with an electro-optical deflecting element including an electro-optical crystal in which a refractive index gradient is induced by injecting electric current.

摘要翻译： 可以以等于视频速率或更高的扫描速度实现用于显微镜观察的所需场数和数值孔径，并且还可以以简单的配置改变扫描速度。 本发明提供一种包括激光光源的激光显微镜; 扫描单元，被配置为用从所述激光光源发射的激光扫描样本; 以及配置为将由扫描单元扫描的激光聚焦在样本上的物镜。 扫描单元设置有电光偏转元件，其包括通过注入电流而引起折射率梯度的电光晶体。

公开(公告)号：US07709773B2

公开(公告)日：2010-05-04

申请号：US12060432

申请日：2008-04-01

申请人： Yusuke Yamashita ,  Junichi Okada ,  Makio Ueno

发明人： Yusuke Yamashita ,  Junichi Okada ,  Makio Ueno

IPC分类号： G02B7/04 ,  G02B27/40 ,  G02B27/64

CPC分类号： G01N21/6456 ,  G02B2207/113

摘要： A clear fluorescence image is easily obtained irrespective of variation of scattering due to variation of the depth of a focus position in a specimen. A scanning optical device includes a laser light source for emitting laser light, a scanning unit for scanning the laser light emitted from the laser light source on the specimen, a focus depth adjusting unit for adjusting the depth of the focus position in the specimen of the laser light to be scanned, a fluorescence detector for detecting fluorescence generated from the focus position of the laser light in the specimen, a reference depth information storage unit for storing the absolute height of a predetermined reference depth of the focus position of the laser light in the specimen by the focus depth adjusting unit, and a hardware setting storage unit for storing the relative height to the absolute height of the reference depth at each focus position of the laser light and at least one set value of the laser light source, the scanning unit, and the fluorescence detector in association with each other.

摘要翻译： 不管由于样品中的焦点位置的深度的变化而导致的散射变化，容易获得清晰的荧光图像。 扫描光学装置包括用于发射激光的激光源，用于扫描从激光光源发射的激光的扫描单元，用于调整样本中的焦点位置的深度的焦点深度调节单元， 要扫描的激光，用于检测从样本中的激光的聚焦位置产生的荧光的荧光检测器，用于存储激光的聚焦位置的预定参考深度的绝对高度的参考深度信息存储单元， 通过焦点深度调整单元的样本，以及硬件设置存储单元，用于存储与激光的每个聚焦位置处的参考深度的绝对高度相关的相对高度和激光源的至少一个设定值，扫描 单位和荧光检测器相互关联。

公开(公告)号：US20050263690A1

公开(公告)日：2005-12-01

申请号：US11141517

申请日：2005-05-31

申请人： Akinori Araya ,  Makio Ueno

发明人： Akinori Araya ,  Makio Ueno

IPC分类号： G02B21/00 ,  G02B26/10 ,  H01J3/14

CPC分类号： G02B26/101 ,  G02B21/002 ,  G02F1/11 ,  G02F1/33

摘要： A laser scanning microscope includes an acousto-optic element or an electro-optic element in an illumination optical system which introduces a laser beam from a laser beam source of the laser scanning microscope into a microscope. The laser scanning microscope further includes a beam expander disposed between the laser beam source and the acousto-optic element or the electro-optic element.

摘要翻译： 激光扫描显微镜包括在激光扫描显微镜的激光束源将激光束引入显微镜的照明光学系统中的声光元件或电光元件。 激光扫描显微镜还包括设置在激光束源和声光元件或电光元件之间的光束扩展器。

公开(公告)号：US07342219B2

公开(公告)日：2008-03-11

申请号：US11141517

申请日：2005-05-31

申请人： Akinori Araya ,  Makio Ueno

发明人： Akinori Araya ,  Makio Ueno

IPC分类号： H01J3/14 ,  G02B21/06

CPC分类号： G02B26/101 ,  G02B21/002 ,  G02F1/11 ,  G02F1/33

摘要： A laser scanning microscope includes an acousto-optic element or an electro-optic element in an illumination optical system which introduces a laser beam from a laser beam source of the laser scanning microscope into a microscope. The laser scanning microscope further includes a beam expander disposed between the laser beam source and the acousto-optic element or the electro-optic element.

摘要翻译： 激光扫描显微镜包括在激光扫描显微镜的激光束源将激光束引入显微镜的照明光学系统中的声光元件或电光元件。 激光扫描显微镜还包括设置在激光束源和声光元件或电光元件之间的光束扩展器。

公开(公告)号：US20080251689A1

公开(公告)日：2008-10-16

申请号：US12060432

申请日：2008-04-01

申请人： Yusuke YAMASHITA ,  Junichi Okada ,  Makio Ueno

发明人： Yusuke YAMASHITA ,  Junichi Okada ,  Makio Ueno

IPC分类号： G02B27/40

CPC分类号： G01N21/6456 ,  G02B2207/113

摘要： A clear fluorescence image is easily obtained irrespective of variation of scattering due to variation of the depth of a focus position in a specimen. A scanning optical device includes a laser light source for emitting laser light, a scanning unit for scanning the laser light emitted from the laser light source on the specimen, a focus depth adjusting unit for adjusting the depth of the focus position in the specimen of the laser light to be scanned, a fluorescence detector for detecting fluorescence generated from the focus position of the laser light in the specimen, a reference depth information storage unit for storing the absolute height of a predetermined reference depth of the focus position of the laser light in the specimen by the focus depth adjusting unit, and a hardware setting storage unit for storing the relative height to the absolute height of the reference depth at each focus position of the laser light and at least one set value of the laser light source, the scanning unit, and the fluorescence detector in association with each other.

摘要翻译： 不管由于样品中的焦点位置的深度的变化而导致的散射变化，容易获得清晰的荧光图像。 扫描光学装置包括用于发射激光的激光源，用于扫描从激光光源发射的激光的扫描单元，用于调整样本中的焦点位置的深度的焦点深度调节单元， 要扫描的激光，用于检测从样本中的激光的聚焦位置产生的荧光的荧光检测器，用于存储激光的聚焦位置的预定参考深度的绝对高度的参考深度信息存储单元， 通过焦点深度调整单元的样本，以及硬件设置存储单元，用于存储与激光的每个聚焦位置处的参考深度的绝对高度相关的相对高度和激光源的至少一个设定值，扫描 单位和荧光检测器相互关联。