公开(公告)号：US08092670B2

公开(公告)日：2012-01-10

申请号：US11992858

申请日：2006-09-28

申请人： Makoto Bekki ,  Hitoshi Ohara ,  Shuji Sonezaki ,  Koki Kanehira ,  Yumi Ogami ,  Hiroshi Ishikawa ,  Yumi Osaki ,  Hiromasa Tokudome ,  Yoko Yamada ,  Masahiro Miyauchi

发明人： Makoto Bekki ,  Hitoshi Ohara ,  Shuji Sonezaki ,  Koki Kanehira ,  Yumi Ogami ,  Hiroshi Ishikawa ,  Yumi Osaki ,  Hiromasa Tokudome ,  Yoko Yamada ,  Masahiro Miyauchi

IPC分类号： G01N27/26 ,  G01N33/48 ,  G01N33/487 ,  G01N33/49 ,  G01N33/493 ,  G01N33/50 ,  G01N33/53 ,  G01N33/533

CPC分类号： G01N33/5438

摘要： A method, an electrode, a measuring cell, and a measuring device are disclosed which can detect and quantitatively determine an analyte having specific bonding properties, in a highly sensitive, simple and accurate manner using photocurrent. This method comprises contacting a working electrode and a counter electrode with an electrolyte medium, wherein the working electrode has an analyte immobilized thereon through a probe substance and wherein the analyte is bonded to a sensitizing dye; irradiating the working electrode with light to photoexcite the sensitizing dye; and detecting photocurrent flowing between the working electrode and the counter electrode, wherein the photocurrent is generated by transfer of electrons from the photoexcited sensitizing dye to the working electrode. The working electrode comprises an electron accepting layer comprising an electron accepting substance capable of accepting electrons released from the sensitizing dye in response to photoexcitation, wherein the probe substance is supported on a surface of the electron accepting layer. The electron accepting substance is an oxide semiconductor having an energy level lower than that of a lowest unoccupied molecular orbit (LUMO) of the sensitizing dye. The electrolyte medium comprises an electrolyte and at least one solvent selected from an aprotic solvent and a protic solvent, wherein the electrolyte comprises a salt capable of providing an oxidized sensitizing dye with electrons.

摘要翻译： 公开了一种方法，电极，测量单元和测量装置，其可以使用光电流以高灵敏度，简单和准确的方式检测和定量地确定具有特定粘合特性的分析物。 该方法包括使工作电极和对电极与电解质介质接触，其中工作电极通过探针物质固定在其上的分析物，并且其中分析物与致敏染料结合; 用光照射工作电极对光敏染料进行光刺激; 并且检测在工作电极和对电极之间流动的光电流，其中通过将电子从光激发敏化染料转移到工作电极而产生光电流。 工作电极包括电子接受层，其包含能够响应于光激发而接受从感光染料释放的电子的电子接受物质，其中探针物质被负载在电子接受层的表面上。 电子接受物质是具有比敏化染料的最低未占据分子轨道（LUMO）的能级更低的氧化物半导体。 电解质介质包括电解质和至少一种选自非质子溶剂和质子溶剂的溶剂，其中电解质包含能够提供氧化的致敏染料与电子的盐。

公开(公告)号：US20090294305A1

公开(公告)日：2009-12-03

申请号：US11992858

申请日：2006-09-28

申请人： Makoto Bekki ,  Hitoshi Ohara ,  Shuji Sonezaki ,  Koki Kanehira ,  Yumi Ogami ,  Hiroshi Ishikawa ,  Yumi Osaki ,  Hiromasa Tokudome ,  Yoko Yamada ,  Masahiro Miyauchi

发明人： Makoto Bekki ,  Hitoshi Ohara ,  Shuji Sonezaki ,  Koki Kanehira ,  Yumi Ogami ,  Hiroshi Ishikawa ,  Yumi Osaki ,  Hiromasa Tokudome ,  Yoko Yamada ,  Masahiro Miyauchi

IPC分类号： G01N27/26 ,  G01F1/64

CPC分类号： G01N33/5438

摘要： A method, an electrode, a measuring cell, and a measuring device are disclosed which can detect and quantitatively determine an analyte having specific bonding properties, in a highly sensitive, simple and accurate manner using photocurrent. This method comprises contacting a working electrode and a counter electrode with an electrolyte medium, wherein the working electrode has an analyte immobilized thereon through a probe substance and wherein the analyte is bonded to a sensitizing dye; irradiating the working electrode with light to photoexcite the sensitizing dye; and detecting photocurrent flowing between the working electrode and the counter electrode, wherein the photocurrent is generated by transfer of electrons from the photoexcited sensitizing dye to the working electrode. The working electrode comprises an electron accepting layer comprising an electron accepting substance capable of accepting electrons released from the sensitizing dye in response to photoexcitation, wherein the probe substance is supported on a surface of the electron accepting layer. The electron accepting substance is an oxide semiconductor having an energy level lower than that of a lowest unoccupied molecular orbit (LUMO) of the sensitizing dye. The electrolyte medium comprises an electrolyte and at least one solvent selected from an aprotic solvent and a protic solvent, wherein the electrolyte comprises a salt capable of providing an oxidized sensitizing dye with electrons.

摘要翻译： 公开了一种方法，电极，测量单元和测量装置，其可以使用光电流以高灵敏度，简单和准确的方式检测和定量地确定具有特定粘合特性的分析物。 该方法包括使工作电极和对电极与电解质介质接触，其中工作电极通过探针物质固定在其上的分析物，并且其中分析物与致敏染料结合; 用光照射工作电极对光敏染料进行光刺激; 并且检测在工作电极和对电极之间流动的光电流，其中通过将电子从光激发敏化染料转移到工作电极而产生光电流。 工作电极包括电子接受层，其包含能够响应于光激发而从感光染料中释放的电子的受电子物质，其中探针物质被负载在电子接受层的表面上。 电子接受物质是具有比敏化染料的最低未占据分子轨道（LUMO）的能级更低的氧化物半导体。 电解质介质包括电解质和至少一种选自非质子溶剂和质子溶剂的溶剂，其中电解质包含能够提供氧化的致敏染料与电子的盐。