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1.发明授权Cultured muscle cells with high metabolic activity and method for production of the cultured muscle cells 有权具有高代谢活性的培养肌肉细胞和培养的肌肉细胞的生产方法公开(公告)号：US07829334B2
公开(公告)日：2010-11-09
申请号：US11524375
申请日：2006-09-21
申请人： Makoto Kanzaki , Taku Nedachi , Hideaki Fujita
发明人： Makoto Kanzaki , Taku Nedachi , Hideaki Fujita
CPC分类号： C12N13/00 , C12M21/08 , C12M25/04 , C12M35/02 , C12M35/08 , C12N5/0658 , C12N2500/02 , C12N2533/52 , C12N2533/54 , G01N33/5061 , G01N33/6872
摘要： The object of the present invention is to provide a method of preparing excellent cultured muscle cells having high metabolic capacity and insulin responsiveness, and further provide a method for the measurement of sensitive metabolic capacity using the cells. The present invention is a method of preparing myotube cells, comprising a step (1) of culturing myoblast cells, a step (2) of differentiation-inducing the myotube cells into the myoblast cells in a culture medium with a high content of amino acids, and a step (3) of applying an electric pulse to the differentiation-induced myotube cells.
摘要翻译： 本发明的目的是提供一种制备具有高代谢能力和胰岛素反应性的优良培养肌细胞的方法，并且还提供了使用细胞测量敏感代谢能力的方法。 本发明是一种制备肌管细胞的方法，包括培养成肌细胞的步骤（1），将具有高含量氨基酸的培养基中的肌管细胞分化为成肌细胞的步骤（2） 以及将电脉冲施加到分化诱导的肌管细胞的步骤（3）。
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公开(公告)号：US20080299086A1
公开(公告)日：2008-12-04
申请号：US11524375
申请日：2006-09-21
申请人： Makoto Kanzaki , Taku Nedachi , Hideaki Fujita
发明人： Makoto Kanzaki , Taku Nedachi , Hideaki Fujita
CPC分类号： C12N13/00 , C12M21/08 , C12M25/04 , C12M35/02 , C12M35/08 , C12N5/0658 , C12N2500/02 , C12N2533/52 , C12N2533/54 , G01N33/5061 , G01N33/6872
摘要： The object of the present invention is to provide a method of preparing excellent cultured muscle cells having high metabolic capacity and insulin responsiveness, and further provide a method for the measurement of sensitive metabolic capacity using the cells. Moreover, its purpose is to provide a culture system/culture apparatus that can smoothly translocate such highly advanced cultured muscle cells intact to activity evaluation systems of a number of drugs. Moreover, the object of the present invention is to provide cultured muscle cells that are very suitable for measurement of the membrane-translocation activity of GLUT4 in an extraneous stimulus-dependent manner such as insulin, etc., and to provide a method for the measurement of the membrane-translocation activity of GLUT4 using the cells. The present invention is a method of preparing myotube cells, comprising a step (1) of culturing myoblast cells, a step (2) of differentiation-inducing the myotube cells into the myoblast cells in a culture medium with a high content of amino acids, and a step (3) of applying an electric pulse to the differentiation-induced myotube cells, and a method for the measurement of insulin-dependent sugar uptake using the myotube cells prepared by said method, and relates to the method for the measurement, comprising applying insulin stimulation by culturing the cells in a culture medium containing insulin, culturing the cells in the culture medium further supplemented with sugar, and measuring the sugar uptake. Furthermore, the present invention relates to a differentiation-type culture myotube cell constitutively expressing a recombinant GLUT4 having a labeled substance at its extra-cellular site, which is prepared by co-culturing wild-type myoblast cells and recombinant myoblast cells constitutively expressing said recombinant GLUT4, and a method for the measurement of membrane-translocation activity of the recombinant GLUT4 using the cells, and particularly a method for the measurement of insulin-dependent sugar uptake activity.
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